Dog SPD(Surfactant Associated Protein D) ELISA Kit
Rif. EK-ELK9773
| 48T | 403,00 € | ||
| 96T | 577,00 € |
Informazioni sul prodotto
- SFTPD; COLEC7; PSP-D; SFTP4; SP-D; Pulmonary Surfactant Protein D; Collectin-7; Lung surfactant protein D
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog SPD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog SPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog SPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog SPD in the samples is then determined by comparing the OD of the samples to the standard curve.
Proprietà chimiche
Richiesta tecnica su: EK-ELK9773 Dog SPD(Surfactant Associated Protein D) ELISA Kit
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