CAS 1158-17-4

o-Nitrofenil β-D-tiogalactosídeo

Descrição:

o-Nitrofenil β-D-tiogalactosídeo (comumente abreviado como ONPG) é um composto sintético amplamente utilizado como substrato em ensaios bioquímicos, particularmente no estudo da atividade enzimática, como a β-galactosidase. É um sólido cristalino incolor que é solúvel em água e solventes orgânicos. O ONPG é caracterizado por sua capacidade de liberar o-nitrofenol após hidrólise, que pode ser medida quantitativamente devido à sua cor amarela, tornando-se um indicador útil em ensaios enzimáticos. O composto apresenta uma ligação tiogalactosídica, que é suscetível à clivagem enzimática, permitindo que os pesquisadores estudem a cinética e os mecanismos das enzimas glicosidases. Sua estrutura molecular inclui um grupo nitrofenila, que contribui para suas propriedades espectroscópicas. O ONPG é frequentemente utilizado em microbiologia para a detecção da fermentação de lactose e em biologia molecular para a avaliação da expressão gênica. Precauções de segurança devem ser observadas ao manusear ONPG, assim como com muitas substâncias químicas, devido à potencial toxicidade e impacto ambiental.

Fórmula: C₁₂H₁₅NO₇S

InChI: InChI=1/C12H15NO7S/c14-5-7-9(15)10(16)11(17)12(20-7)21-8-4-2-1-3-6(8)13(18)19/h1-4,7,9-12,14-17H,5H2

Chave InChI: InChIKey=SZAOZNVCHHBUDZ-RUXWNWLUSA-N

SMILES: S([C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O)C2=C(N(=O)=O)C=CC=C2

Sinónimos:

• 2-Nitrophenyl 1-Thiohexopyranoside
• 2-Nitrophenyl 1-thio-β-<span class="text-smallcaps">D</span>-galactopyranoside
• 2-Nitrophenyl-β-<span class="text-smallcaps">D</span>-thiogalactopyranoside
• 2-nitrophenyl 1-thio-beta-D-galactopyranoside
• Galactopyranoside, o-nitrophenyl 1-thio-
• Galactopyranoside, o-nitrophenyl 1-thio-, β-<span class="text-smallcaps">D</span>-
• Thio-o-nitrophenyl-β-<span class="text-smallcaps">D</span>-galactopyranoside
• beta-D-Galactopyranoside, 2-nitrophenyl 1-thio-
• o-Nitrophenol beta-thiogalactoside
• o-Nitrophenyl 1-thio-beta-D-galactopyranoside
• o-Nitrophenyl 1-thio-β-<span class="text-smallcaps">D</span>-galactoside
• o-Nitrophenyl β-<span class="text-smallcaps">D</span>-thiogalactoside
• β-<span class="text-smallcaps">D</span>-Galactopyranoside, 2-nitrophenyl 1-thio-
• Thio-o-nitrophenyl-β-D-galactopyranoside
• Galactopyranoside, o-nitrophenyl 1-thio-, β-D-
• 2-Nitrophenyl 1-thio-β-D-galactopyranoside
• β-D-Galactopyranoside, 2-nitrophenyl 1-thio-
• ONP-1-THIO-BETA-D-GAL
• O-NITROPHENYLSULFENYL-1-THIO-BETA-D-GALACTOSIDE
• 2-Nitrophenyl-beta-D-thiogalactopyranoside,98%
• o-Nitrophenylsulfenyl-1-thio-β-D-galactoside
• O-NITROPHENYL-1-THIO-BETA-D-GALACTOSIDE
• 2-Nitrophenylb-D-thiogalactopyranoside
• 1-S-(2-Nitrophenyl)-1-thio-β-D-galactopyranose
• RARECHEM AH BS 0066
• 2-(hydroxymethyl)-6-[(2-nitrophenyl)thio]oxane-3,4,5-triol
• O-NITROPHENYL-1-THIO-BETA-D-GALACTOPYRANOSIDE USP/EP/BP
• O-NITROPHENYL-1-THIO-BETA-D-GALACTOPYRANOSID
• 1-(2-Nitrophenylthio)-1-deoxy-β-D-galactopyranose
• 2-NITROPHENYL-BETA-D-THIOGALACTOPYRANOSIDE
• O-NITROPHENYL-BETA-D-THIOGALACTOPYRANOSIDE
• O-NITROPHENYL 1-THIO-B-D-*GALACTOPYRANOS IDE
• 2-Nitrophenyl β-D-thiogalactopyranoside
• o-Nitrophenyl-1-thio-β-D-galactopyranoside≥ 99% (TLC)

β-D-Galactopyranoside, 2-nitrophenyl 1-thio-

CAS: 1158-17-4

Fórmula: C₁₂H₁₅NO₇S

Pureza: 98%

Cor e Forma: Solid

Peso molecular: 317.3150

Ref: IN-DA000GWC

2-Nitrophenyl b-D-thiogalactopyranoside

CAS: 1158-17-4

Ref: 7W-GC2622

2-Nitrophenyl 1-thio-β-D-galactopyranoside

CAS: 1158-17-4

2-Nitrophenyl 1-thio-β-D-galactopyranoside

Peso molecular: 317.32g/mol

Ref: 54-BIN1245

2-Nitrophenyl-β-D-thiogalactopyranoside

CAS: 1158-17-4

2-Nitrophenyl-beta-D-thiogalactopyranoside is a chromogenic substrate used to detect the presence of galactosidase enzymes in various biological and biochemical assays. It consists of a nitrophenyl group attached to a galactose sugar molecule through a thioglycosidic bond. When acted upon by enzymes such as galactosidases, it releases the colored nitrophenyl group, which can be detected using spectrophotometry. This substrate is commonly used in assays for the detection of LacZ activity in bacterial cultures, as lacZ encodes for β-galactosidase.

Fórmula: C₁₂H₁₅NO₇S

Pureza: Min. 98 Area-%

Cor e Forma: Yellow Powder

Peso molecular: 317.32 g/mol

Ref: 3D-EN03315

2-Nitrophenyl-1-thio-β-D-galactopyranoside

CAS: 1158-17-4

2-Nitrophenyl-1-thio-beta-D-galactopyranoside is a nitroaryl compound that can be synthesized from beta-D-galactopyranosides and nitric acid. It has a molecular conformation that is stabilized by hydrogen bonds, which allows it to form supramolecular assemblies with other molecules. The nitro group in this molecule is responsible for the formation of the nitro group in 1,2,4,5,-tetranitrobenzene.

Fórmula: C₁₂H₁₅NO₇S

Peso molecular: 317.32 g/mol

Ref: 3D-N-5100