CAS 142988-22-5

DABCYL-.gamma.-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS

Descrição:

DABCYL-.gamma.-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS é um composto químico baseado em peptídeos que serve como uma sonda fluorescente, comumente utilizada em pesquisa bioquímica e biofísica. A estrutura apresenta um grupo DABCYL, que atua como um quencher, e um grupo EDANS, um doador fluorescente, permitindo aplicações de transferência de energia por ressonância de Förster (FRET). Este composto é caracterizado por sua sequência específica de aminoácidos, que inclui vários resíduos hidrofóbicos e polares, contribuindo para sua estabilidade e propriedades de interação em sistemas biológicos. A presença de DABCYL e EDANS permite o estudo de interações de peptídeos, mudanças conformacionais e atividade enzimática por meio de medições de fluorescência. Suas propriedades únicas o tornam valioso em ensaios para monitorar a atividade proteolítica e outras interações moleculares. Além disso, o design do composto permite personalização em aplicações de pesquisa, tornando-o uma ferramenta versátil nos campos da biologia molecular e bioquímica.

Fórmula: C₉₀H₁₂₀N₂₂O₁₆S

InChI: InChI=1/C90H120N22O16S/c1-12-53(7)76(87(122)103-71(43-61-46-94-49-98-61)84(119)107-77(55(9)113)79(115)66-35-34-64-65(80(66)129(126,127)128)22-17-23-67(64)96-38-37-92)106-86(121)75(52(5)6)105-83(118)68(40-51(3)4)100-82(117)70(42-60-45-93-48-97-60)101-81(116)69(41-56-20-15-14-16-21-56)102-85(120)73-24-19-39-111(73)90(125)72(44-62-47-95-50-99-62)104-88(123)78(54(8)13-2)112(74(114)25-18-36-91)89(124)57-26-28-58(29-27-57)108-109-59-30-32-63(33-31-59)110(10)11/h14-17,20-23,26-35,45-55,68-73,75-78,96,113H,12-13,18-19,24-25,36-44,91-92H2,1-11H3,(H,93,97)(H,94,98)(H,95,99)(H,100,117)(H,101,116)(H,102,120)(H,103,122)(H,104,123)(H,105,118)(H,106,121)(H,107,119)(H,126,127,128)

SMILES: CCC(C)C(C(=NC(Cc1cnc[nH]1)C(=NC(C(C)O)C(=O)c1ccc2c(cccc2NCCN)c1S(=O)(=O)O)O)O)N=C(C(C(C)C)N=C(C(CC(C)C)N=C(C(Cc1cnc[nH]1)N=C(C(Cc1ccccc1)N=C(C1CCCN1C(=O)C(Cc1cnc[nH]1)N=C(C(C(C)CC)N(C(=O)CCCN)C(=O)c1ccc(cc1)N=Nc1ccc(cc1)N(C)C)O)O)O)O)O)O

Sinónimos:

• 4-(4-Dimethylaminophenylazo)benzoyl-4-aminobutanoyl-isoleucyl-histidyl-prolyl-phenylalanyl-histidyl-leucyl-valyl-isoleucyl-histidyl-threonyl-5-((2-aminoethyl)amino)naphthalene-1-sulfonic acid
• Dabcyl-GABA-ile-his-pro-phe-his-leu-val-ile-his-thr-edans
• Dabgab-ipfit-edans
• N-(4-((4-((4-(Dimethylamino)phenyl)azo)benzoyl)amino)-1-oxobutyl)-L-isoleucyl-L-histidyl-L-prolyl-L-phenylalanyl-L-histidyl-L-leucyl-L-valyl-L-isoleucyl-L-histidyl-N-(2-((5-sulfo-1-naphthalenyl)amino)ethyl)-L-threoninamide
• L-Threoninamide, N-(4-((4-((4-(dimethylamino)phenyl)azo)benzoyl)amino)-1-oxobutyl)-L-isoleucyl-L-histidyl-L-prolyl-L-phenylalanyl-L-histidyl-L-leucyl-L-valyl-L-isoleucyl-L-histidyl-N-(2-((5-sulfo-1-naphthalenyl)amino)ethyl)-

Renin FRET Substrate I

CAS: 142988-22-5

Renin FRET Substrate I, designed for human renin, contains angiotensinogen's N-terminal cleavage site.

Fórmula: C₉₀H₁₂₀N₂₂O₁₆S

Peso molecular: 1798.15

Ref: TM-T38900

Dabcyl-γ-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Edans

Produto Controlado

CAS: 142988-22-5

Dabcyl-γ-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Edans is an angiotensinogen peptide. It has been used as a substrate for Renin and assayed by fluorescence to study the binding affinity of protease inhibitors. Dabcyl is a fluorescent label that can be used in peptide and biochemicals assays, including fluorescence assay. Dabcyl is soluble in water and has little fluorescence quenching with other compounds, making it ideal for use in these applications.

Fórmula: C₉₀H₁₂₀N₂₂O₁₆S

Pureza: Min. 95%

Peso molecular: 1,798.16 g/mol

Ref: 3D-SFQ-3731-PI

DABCYL-gamma-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS trifluoroacetate salt

Produto Controlado

CAS: 142988-22-5

DABCYL-gamma-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr (DABCYL) is a fluorescent substrate that has been used to study the kinetics of peptide hydrolysis by proteases. It is an amino acid sequence that is present in angiotensinogen, which is a blood protein involved in regulating blood pressure. The DABCYL group on the terminal amino acid of the peptide provides a highly fluorescent molecule that can be excited at wavelengths longer than 400 nm. This fluorophore can also be used as a donor for fluorescence resonance energy transfer (FRET) with other fluorophores, such as EDANS, which has been shown to have high affinity for DABCYL. DABCYL can be used to measure enzyme activity or inhibition and has been found to be sensitive enough to detect changes due to dilutions at concentrations as low as 10 nM.

Fórmula: C₉₀H₁₂₀N₂₂O₁₆S

Pureza: Min. 95%

Peso molecular: 1,798.12 g/mol

Ref: 3D-FD110909