CAS 72-57-1

Azul de tripano

Descrição:

Azul de tripano é um corante sintético comumente usado em pesquisas biológicas e médicas, particularmente para ensaios de viabilidade celular. É um corante diazo que aparece como um pó ou solução azul escuro e é solúvel em água. O corante penetra seletivamente em células mortas, permitindo que os pesquisadores distingam entre células viáveis e não viáveis sob um microscópio. Essa característica se deve à integridade da membrana comprometida das células mortas, que permite a entrada do corante, enquanto as células vivas o excluem. Azul de tripano é frequentemente usado em conjunto com hemocitômetros para contagem de células em cultura. Embora geralmente seja considerado seguro para uso em laboratório, é importante manuseá-lo com cuidado, pois pode ser um irritante potencial e é classificado como um possível carcinógeno. Além de suas aplicações em biologia celular, Azul de tripano também tem sido usado em histologia e como agente de coloração em vários procedimentos diagnósticos. No geral, sua capacidade de fornecer uma distinção visual entre células vivas e mortas o torna uma ferramenta valiosa na pesquisa em biologia celular.

Fórmula: C₃₄H₂₈N₆O₁₄S₄·4Na

InChI: InChI=1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;

Chave InChI: InChIKey=UBHCTCVOHFAILV-UHFFFAOYSA-N

SMILES: OC=1C2=C(C=C(S(=O)(=O)O)C1N=NC3=C(C)C=C(C=C3)C4=CC(C)=C(N=NC=5C(S(=O)(=O)O)=CC6=C(C5O)C(N)=CC(S(=O)(=O)O)=C6)C=C4)C=C(S(=O)(=O)O)C=C2N.[Na]

Sinónimos:

• 2,7-Naphthalenedisulfonic acid, 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(2,1-diazenediyl)]bis[5-amino-4-hydroxy-, sodium salt (1:4)
• 2,7-Naphthalenedisulfonic acid, 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxy-, tetrasodium salt
• 3,3'-[(3,3'-Dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphtalene-2,7-disulfonate] de tetrasodium
• 3,3'-[(3,3'-Dimetil[1,1'-Bifenil]-4,4'-Diil)Bis(Azo)]Bis[5-Amino-4-Hidroxinaftaleno-2,7-Disulfonato] De Tetrasodio
• Amanil Sky Blue R
• Bencidal Blue 3B
• Benzamine blue
• Benzanil Blue 3BN
• Benzo Blue 3BS
• Benzo blue
• Blue 3B
• Blue EMB
• Brasilamina Blue 3B
• Chloramine Blue 3B
• Chrome Leather Blue 3B
• Congo blue
• Cresotine Blue 3B
• Diamine Blue 3B
• Diamineblue
• Diaphtamine Blue TH
• Diazine Blue 3B
• Diazol Blue 3B
• Diphenyl Blue
• Diphenyl Blue 3B
• Direct Blue 14
• Direct Blue 3B
• Direct Blue M 3B
• Hispamin Blue 3BX
• Naphthylamine blue
• Niagara Blue
• Niagara Blue 3B
• Paramine Blue 3B
• Pontamine Blue 3BX
• Sodium ditolyldisazobis-8-amino-1-naphthol-3,6-disulfonate
• Tetranatrium-3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalin-2,7-disulfonat]
• Tetrasodium 3,3'-[(3,3'-Dimethyl[1,1'-Biphenyl]-4,4'-Diyl)Bis(Azo)]Bis[5-Amino-4-Hydroxynaphthalene-2,7-Disulfonate]
• Tetrasodium 3,3'-[(3,3'-Dimethyl[1,1'-Biphenyl]-4,4'-Diyl)Bis(Azo)]Bis[5-Amino-4-Hydroxynaphthalene-2,7-Disulphonate]
• Trypan (Congo) Blue
• Trypan Blue (Dye Cont. Ca. 60%)
• Trypan Blue BPC
• Trypan Blue solution
• Trypane blue
• tetrasodium (3Z,3'Z)-3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)di(1Z)hydrazin-2-yl-1-ylidene]bis(5-amino-4-oxo-3,4-dihydronaphthalene-2,7-disulfonate)
• tetrasodium 3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)di(E)diazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonate)
• C.I. Direct Blue 14, tetrasodium salt
• C.I. Direct Blue 14, tetrasodium salt (8CI)
• C.I. Direct Blue 14
• C.I. Direct Blue 14, tetrasodium salt
• C.I. 23850
• Direct Blue 14 (C.I.)

Direct Blue 14

CAS: 72-57-1

Fórmula: C₃₄H₂₄N₆Na₄O₁₄S₄

Cor e Forma: Amber to Dark green to Black powder to crystal

Peso molecular: 960.79

Ref: 3B-T0556

Trypan Blue, dye content >60%

CAS: 72-57-1

Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. Used in dye exclusion studies in cell culture. A viability dye for quantifying live versus dead cells with permeable membranes, blue. It is also used to identify islet cell clust

Fórmula: C₃₄H₂₄N₆Na₄O₁₄S₄

Pureza: 60%

Cor e Forma: Dark purple to dark brown or black, Powder

Peso molecular: 960.79

Ref: 02-A18600

2,7-Naphthalenedisulfonic acid,3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxy-, tetrasodium salt

CAS: 72-57-1

Fórmula: C₃₄H₂₄N₆Na₄O₁₄S₄

Pureza: 70%

Cor e Forma: Solid

Peso molecular: 960.8052

Ref: IN-DA00IC7M

Trypan Blue (C.I. 23850); 0.4% solution

CAS: 72-57-1

Fórmula: C₃₄H₂₄N₆Na₄O₁₄S₄

Cor e Forma: Blue to very dark blue liquid

Peso molecular: 960.82

Ref: 7W-GT0822

Trypan Blue

CAS: 72-57-1

Trypan Blue

Pureza: 80%

Peso molecular: 960.81g/mol

Ref: 54-OR1015400

Diphenyl Blue

CAS: 72-57-1

Diphenyl Blue

Peso molecular: 960.81g/mol

Ref: 54-BUP19760

Trypan Blue (C.I. 23850)

CAS: 72-57-1

Fórmula: C₃₄H₂₄N₆Na₄O₁₄S₄

Cor e Forma: Dark green, brown or black powder

Peso molecular: 960.82

Ref: 7W-GT3970

Trypan Blue

CAS: 72-57-1

Fórmula: C₃₄H₂₈N₆NaO₁₄S₄

Peso molecular: 895.86

Ref: 4Z-D-164054

Diphenyl Blue

CAS: 72-57-1

Diphenyl Blue (Direct Blue 14) (Direct Blue 14) is used as a dye for a group of azo dye.

Fórmula: C₃₄H₂₄N₆Na₄O₁₄S₄

Pureza: ≥98%

Cor e Forma: Blueish Grey Powder

Peso molecular: 960.81

Ref: TM-T18979

Trypan blue

CAS: 72-57-1

Dye used in microscopy for assessment of cell viability

Fórmula: C₃₄H₂₄N₆O₁₄S₄Na₄

Cor e Forma: Powder

Peso molecular: 960.81 g/mol

Ref: 3D-FT47140

Trypan Blue 0.4% Solution

CAS: 72-57-1

Cor e Forma: Dark blue, Liquid

Ref: SR-12788

Trypan Blue

CAS: 72-57-1

Fórmula: C₃₄H₂₄N₆Na₄O₁₄S₄

Cor e Forma: Brown to black, Crystalline powder

Peso molecular: 960.80

Ref: SR-58026

Trypan Blue for tissue culture

CAS: 72-57-1

Fórmula: C₃₄H₂₄N₆Na₄O₁₄S₄

Cor e Forma: Brown to black, Powder

Peso molecular: 960.80

Ref: SR-31591

Trypan Blue (Technical Grade)

CAS: 72-57-1

Applications Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples. Dyes and metabolites.
References Bonnefille, P. et al. 2010. Fundam Clin Pharmacol. PMID: 21121944; Han, T.H. et al. 2010. J Acupunct Meridian Stud. 3(4): 241-248. PMID: 21185538; Saadatnia, G. et al. 2010. Trop Biomed. 27(1): 125-130. PMID: 20562822; Hani, H. et al. 2010. Xenotransplantation. 17(6): 469-480. PMID: 21158948

Fórmula: C₃₄H₂₄N₆O₁₄S₄·4Na

Cor e Forma: Neat

Peso molecular: 960.8

Ref: TR-T895523