Substratos fluorogénicos

4-Methylumbelliferyl-2-O-(alpha-L-fucopyranosyl)-beta-L-fucopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl-2-O-(alpha-L-fucopyranosyl)-beta-L-fucopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for diagnosis of fucosidosis (defficiency of L-fucosidase).

Resorufin b-D-cellobioside is a fluorogenic substrate for measurement of cellulase activity. The product of enzyme reaction, resorufin, exhibits fluorescence emission at 585 nm with excitation at 571 nm at or near physiological pH values.

Bis-(4-methylumbelliferyl)phosphate, or bis-4MU-phopshate, is a fluorogenic substrate used to study phosphatase activity, particularly in enzyme-linked immunosorbent assays (ELISAs).

4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-galactopyranoside-6-sulfate potassium salt is a fluorogenic substrate for N-acetylgalactosamine-6-sulfatase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-galactopyranoside-6-sulfate potassium saltas a substrate for measuring the N-acetylgalactosamine-6-sulfatase activity is used for diagnosis of Mucopolysaccharidosis IVA (MPS IVA) (a rare lysosomal storage disorder) and Morquio A syndrome screening.

4-Trifluoromethylumbelliferyl b-D-glucopyranoside is a fluorogenic substrate for beta-glucosidase. Upon hydrolysis, the fluorogenic moiety (blueish) 4-trifluoromethylumbelliferone (also known as TFMU) is released. 4-Trifluoromethylumbelliferyl b-D-glucopyranoside is commonly used in bacterial detection and in Gaucher disease diagnosis.

A substrate used for the diagnosis of Sanfilippo disease C (MPS III C).

(+)-Biotin 4-amidobenzoic acid sodium salt, often referred as (+)-biotin PABA, is a fluorogenic substrate used to measure biotinidase activity, especially in newborn screening programs.

PAIRED PRODUCT AVAILABLE: alpha-L-iduronidase Enzyme, FI179383.
4MU-alpha-L-idopyranosiduronic acid is a fluorogenic substrate for alpha-L-iduronidase. Active alpha-L-iduronidase cleaves the MU moiety from the synthetic substrate, which results in release of a fluorescent signal (Ex 360 nm / Em 455 nm). The reaction is stopped by the addition of sodium carbonate. 4MU-alpha-L-idopyranosiduronic acid is used in alpha-L-iduronidase assays in the context of biochemical characterisation of samples from patients affected by lysosomal storage disease mucopolysaccharidosis I (MPS I).

4-Methylumbelliferyl palmitate, commonly names as 4-MUP, is a fluorescent probe that binds to lipases, enzymes responsible to assist on the hydrolysis of fats. In diagnosis, 4-MUP is used for screening of lysosomal storage diseases (LSD) caused by a deficiency of a single enzyme involved in the metabolism of glycoproteins, lipids or mucopolysaccharides.