Substratos fluorogénicos

A MOSCERDAM® substrate targeting the fucosidase enzyme

4-Methylumbelliferyl-alpha-L-rhamnopyranoside is a fluorogenic substrate for alpha-L-rhamnosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl-alpha-L-rhamnopyranosideas a substrate for measuring the alpha-L-rhamnosidase activity is used for detection of bacterial infection.

6'-(Diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl b-D-glucopyranoside is a fluorogenic substrate that is used in diagnostic applications. It has been shown to be a useful ligand for the determination of enzyme activity, and it can also be used to detect bacterial culture media contaminants. 6'-(Diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl b-D-glucopyranoside is used as an enzyme substrate in chemiluminescent assays, and with other reagents, it can be used for the detection of environmental contaminants. This product has a CAS number of 55426-02-8 and can be found on the website www.sigmaaldrich.com/catalog/product

4-Trifluoromethylumbelliferyl-beta-D-galactopyranoside is a fluorogenic substrate for beta-galactosidase. It is used in the detection of beta-galactosidase activity in cell lysates and culture media, as well as in food testing. This compound has been shown to be highly pure, with a purity greater than 98%. 4-Trifluoromethylumbelliferyl-beta-D-galactopyranoside has also been shown to have high chemiluminescence, making it an ideal candidate for use in diagnostics.

Fluorogenic substrate for proglutamyl peptidase 1 yielding a blue fluorescent solution upon cleavage. Used in identification of Enterococcus spp. and group A streptococci (Streptococcus pyogenes).

The Methionine Assay Kit (Fluorometric) is a kit containing the fluorogenic substrate needed for the determination of total methionine content. The kit is used to detect and quantify methionine in various food products, as well as in environmental samples. This assay can be used to measure free or protein-bound methionine. The fluorometric kit contains a conjugate that binds to the methionine molecule and provides a fluorescent signal proportional to the amount of bound methionine. The fluorescence intensity is measured on a fluorometer and converted into concentrations of methionine per gram of sample.

N-CBZ-L-Lysyl-L-lysyl-L-arginine 7-amido-4-methylcoumarin triacetate is a synthetic peptide substrate used as a fluorogenic substrate for aminopeptidase.

6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-cellotrioside is a fluorogenic substrate for cellulases and beta-glucosidases. After hydrolisis, the fluorophore TFMU is released generating a blue fluorescence.

A sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases. It is used for the detection of C. perfringens because acid phosphatase is a highly specific indicator for C. perfringens.

Lipase targeting MOSCERDAM® substrate

A fluorogenic substrate for the detection of lysosomal enzyme N-acetyl-α-D-galactopyranosiaminidase (NAGA), which is driving the pathogenesis of Schindler disease. Also substrate for endo-α-N-acetylgalactosaminidase (O-glycosidase), which cleaves O-linked core 1 and core 3 disaccharides from glycoproteins.

FLsharp™ - Phosphate, disodium salt is a fluorogenic water soluble substrate for phosphatase enzymes. After cleavage of the substrate by phosphatase activity an insoluble product is formed that is highly fluorescent in the solid state. Upon UV irradiation this very stable fluorophore emits green light in the 530 nm range for an extended period of time. FLsharp™ - Phosphate is a unique substrate - optimized for the detection of phosphatase activity in solid matrices such as agar media, tissuesections or microscopic preparations. FLsharp™ - Phosphate, disodium salt is used in microbiology as asubstrate for bacterial phosphatases. The substrate is suitable to detect the specific phosphatase activity of S. aureus or C. perfringensin food samples or clinical samples. Colonies growing on plating media supplemented with FLsharp™ emit green light upon UV light excitation(Excitation max: 365 nm; emission max: 530 nm). The enzyme product formed from FLsharp™ in agar plating media enables high precision for the localization of the relevant colonies on the plate. As a result small bacteria colonies of phosphatase-positive bacteria can be detected already after a short incubation time.  In cytochemical and histochemical imaging FLsharp™ - Phosphate, disodiumsalt can be used as a substrate for Alkaline Phosphatase (AP)-coupled antibodies. The fluorophore that is released from FLsharp™ - Phosphateby phosphatase activity will precipitate. This insoluble fluorophoreen ables a precise localization of the antibody-bound epitope in cells or tissues under a fluorescence microscope or fluorescence imaging system. The green fluorescence of FLsharp™ can be clearly differentiated from autofluorescence or counterstainings such as (blue) DAPI or (red) Alexafluorophores.

The 4-Methylumbelliferyl b-D-ribofuranoside is a top-notch fluorogenic substrate for glycosidase activity detection. Upon hydrolysis by enzymes, it generates a strongly fluorescent 4-methylumbelliferone molecule, enabling rapid assay results and real-time monitoring of enzymatic reactions. This product is suitable for many biological research applications, such as in vitro analysis, diagnostic testing, and drug discovery. With its high sensitivity and specificity, it delivers consistent and accurate results, making it an essential tool for scientists and researchers.

Fluorogenic substrate for detection of β-D-mannosidase.

3-(4,4-Difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionamido)propyl b -D -lactoside (FDPBP) is a substrate for alkaline phosphatase. It reacts with the enzyme to produce a blue color. FDPBP is used in diagnostic applications such as food testing and bioluminescence.

4-Methylumbelliferyl N,N',N''-triacetyl-beta-D-chitotrioside is a fluorogenic substrate for chitinase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl N,N',N''-triacetyl-beta-D-chitotriosideas a substrate for measuring the chitinase activity is used for fungal infection assays.

Resorufin N-acetyl-b-D-galactosamine is a fluorogenic substrate used as a probe to detect and quantify the activity of N-acetyl-beta-D-galactosaminidase (also known as beta-hexosaminidase or HEX(NAG)). Particularly useful in biomedical research, clinical chemistry, and diagnostics, when the substrate is cleaved, the highly fluorescent resorufin dye is produced that can be detected at approximately 598 nm.