Electroforese
Nesta seção, você encontrará todos os reagentes e materiais necessários para realizar a eletroforese, uma técnica laboratorial vital usada para separar proteínas de acordo com sua massa molecular e carga. Esta técnica é essencial para analisar a composição e pureza de amostras de proteínas, bem como para estudar ácidos nucleicos e outras biomoléculas. Nossa seleção inclui tampões de eletroforese, géis, corantes e marcadores de alta qualidade, juntamente com aparelhos e acessórios projetados para garantir uma separação precisa e eficiente. Esses produtos são cruciais para pesquisa em biologia molecular, bioquímica e genética, proporcionando resultados confiáveis para caracterização de proteínas, análise de expressão gênica e outras aplicações. Na CymitQuimica, fornecemos tudo o que você precisa para realizar eletroforese, apoiando sua pesquisa com precisão e consistência.
Subcategorias de "Electroforese"
Foram encontrados 298 produtos para "Electroforese".
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10X Tris-Tricine-SDS Buffer for molecular biology
The Tris-Tricine-SDS Gel Running Buffer is specifically designed for separating proteins with molecular weights between 1 and 100 kDa, making it particularly effective for resolving proteins smaller than 30 kDa. This approach differs from traditional SDS-PAGE by substituting glycine (pK 9.6) with tricine (pK 8.15), which enhances the stacking and destacking of low molecular weight proteins and improves the resolution of smaller peptides due to the differing pK values. Incorporating urea into the stacking gel allows for the effective separation of two proteins that share the same molecular weight. Additionally, the lower acrylamide concentrations in Tricine gels facilitate the transfer of hydrophobic proteins during Western blotting. Tricine–SDS-PAGE is therefore commonly employed for the separation of lower molecular weight proteins.Cor e Forma:Clear, Colourless, Liquid1M Tris Hydrochloride Buffer (1M Tris HCl) pH-7.2 for molecular biology
Cor e Forma:Clear, Colourless, LiquidBrilliant Blue R-250 for molecular biology
CAS:Fórmula:C45H44N3NaO7S2Cor e Forma:Dark blue with reddish tinge, PowderPeso molecular:825.9810X Phosphate Buffered Saline (PBS) for molecular biology
Phosphate Buffered Saline (PBS) is an essential buffer solution widely utilized in biological research and clinical settings. It plays a critical role in preventing cell rupture or shrinkage caused by osmosis, and its non-toxic formulation is safe for cellular applications. The 10X PBS solution provides a stable pH environment, which is vital for optimal enzyme activity and various biological processes. This stability makes PBS particularly suitable for preparing reagents, conducting protein dialysis, and performing immunohistochemistry, thus ensuring consistent and reliable experimental results. In addition to its applications in cell biology, PBS is also employed in diverse fields, including the development of custom ELISA buffers to optimize antibody-antigen interactions.Cor e Forma:Clear, Colourless, Liquid10X MOPS Buffer for molecular biology
MOPS, or morpholino propanesulfonic acid, is a structural analog of MES, the ethanesulfonic acid. With a pKa value of 6.80-7.20, MOPS serves as an excellent buffer for many biological systems at near-neutral pH. It is extensively used as a buffering agent in molecular biology and biochemistry and has been tested and recommended for use in polyacrylamide gel electrophoresis. Additionally, MOPS is applicable in various bioanalytical methods, including isoelectric focusing, protein assays, and X-ray crystallographic studies. Furthermore, it can be used as an electrophoresis buffer for agarose gel electrophoresis of RNA.Cor e Forma:Clear, Colourless, Liquid20X SSC Buffer pH 6.9-7.1 for molecular biology
The Saline-Sodium Citrate Hybridization Buffer [20X] is a key component in various nucleic acid techniques, ensuring optimal stringency during washing steps in procedures such as Southern blotting, in situ hybridization, DNA microarrays, and Northern blotting. When utilized as 20X SSC, it effectively prevents agarose gels from drying out during vacuum transfers, preserving sample integrity. This solution is specifically formulated to support the diverse needs of end users in nucleic acid hybridizations and blot transfer applications, making it an invaluable resource in molecular biology workflows.Cor e Forma:Clear, Colourless, Solution20X SSPE Buffer pH-7.4 suitable for molecular biology
The 20X SSC Buffer, with a pH between 6.9 and 7.1, is essential for in molecular biology, particularly for Southern and Northern hybridization techniques. This solution is sterilized through a 0.22 µm filter, making it suitable for direct use or for dilution based on experimental requirements. In parallel, 20X SSPE is available as a concentrated buffer intended for nucleic acid hybridizations and blot transfer processes. A significant feature of SSPE is its inclusion of EDTA, which chelates divalent metal ions like Mg²+. This property effectively inhibits DNase activity, helping to preserve the concentrations of probe and target DNA during Southern hybridization, thereby enhancing the accuracy of the results.Cor e Forma:Clear, Colourless, Liquid1X Phosphate Buffered Saline (PBS) for molecular biology
The pH of the buffer is typically maintained within a range of 7.4 ± 0.1, closely resembling the physiological pH found in many biological systems.Cor e Forma:Clear, Colourless, LiquidLithium Lauryl Sulphate extrapure, 99%
CAS:Fórmula:C12H25SO4LiPureza:min. 99%Cor e Forma:White, Crystalline powder, Clear, ColourlessPeso molecular:272.30Albumin Bovine Solution 10% Diluent Solution in PBS (BSA 10% Diluent/Blocking Solution in PBS)
CAS:Cor e Forma:Clear, Pale yellow, LiquidN,N-Methylene Bisacrylamide 3x cryst. for molecular biology, 99.5%
CAS:Fórmula:C7H10N2O2Pureza:min. 99.5%Cor e Forma:White, Crystalline powder, Clear, ColourlessPeso molecular:154.172.6M Tris Hydrochloride Buffer (2.6M Tris HCl) pH-8 for molecular biology
CAS:Cor e Forma:Clear, Colourless, LiquidTris Acetate for molecular biology, 99%
CAS:Fórmula:C4H11NO3·C2H4O2Pureza:min. 99%Cor e Forma:White, Crystalline powder, Clear, ColourlessPeso molecular:181.19
