Compostos relacionados com DNA e RNA

<p>This PCR Ladder has been designed to cover the most common range encountered in PCR amplifications. The range is represented by aesthetically pleasing ladder of five bands (50, 150, 300, 500 and 800 bp) that separate uniformly.BioLit™ SafeDye emits green fluorescence when bound to dsDNA, ssDNA and RNA. This stain has a fluorescence excitation maxima when bound to nucleic acid at approx. 290-320nm and emits at 490nm.This dye replaces Ethidium Bromide (toxic, potential mutagen) for visualisation of DNA or RNA in Agarose gel.</p>

<p>Red Taq DNA Polymerase, a thermostable recombinant DNA polymerase, useful in amplification of DNA fragments upto 6 kb from genomic DNA with high purity and specificity.<br> Sources of template - plant genomic DNA , mouse cDNA, bacterial colony DNA , bacterial plasmid DNA , viral DNA and zygosity PCR templates. No loading buffers or tracking dyes required. Samples may be added directly to an agarose gel after PCR and visualized. </p>

<p>These Ready-to-Load ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis. The concentration of each band is kept uniform to give sharp bands. The ladder consists of 10 linear double-stranded fragments.A single or multiple bands in the ladder are made intense to orient the user to the other bands in the ladder. The 100bp and 300bp fragment are present at increased intensity to allow easy identification. <br> Contains Bromophenol Blue and Xylene Cyanol as the tracking dyes.Size range : 25 to 700 bpMix concentration :104 ng /µl<br> Recommended loading volume is 9 µl (1µg) </p>

<p>These Ready-to-Load ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis.The concentration of each band is kept uniform to give sharp bands. Contains Bromophenol Blue as the tracking dye.Size range : This size marker covers the most common range encountered in PCR and shows an aesthetically pleasing ladder of five bands (50, 150, 300, 500, and 800 bp).<br> Mix concentration :100 ng /µlRecommended loading volume is 10 µl. </p>

<p>Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.</p>

<p>These Ready-to-Load ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis. The concentration of each band is kept uniform to give sharp bands. The ladder consists of 10 linear double-stranded fragments.A single or multiple bands in the ladder are made intense to orient the user to the other bands in the ladder. The 2000bp and 5000bp fragment are present at increased intensity to allow easy identificationSize range : 500 to 10,000 bpContains Bromophenol Blue and Xylene Cyanol as the tracking dyes.Mix concentration : 104 ng /µlRecommended loading volume is 9 µl (1 µg) </p>

<p>One Step RT-qPCR Master Mix is designed for a Highly Efficient cDNA Synthesis and PCR in a single tube having all the components except the primers and template.</p>

<p>The FluroBronze 100bp ladder plus is specially prepared as ready-to-load fluorescent ladder and ideal for determining the size of double-stranded DNA from 100 to 3000 base pairs. The ladder consists of 14 linear double-stranded fragments. The 500bp and 1200bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. The new range of DNA ladders are specially prepared as ready-to-load fluorescent ladders. The flurobronze ladder contains in addition to the tracking dye, a non-carcinogenic nucleic acid stain. The ladder should be loaded onto an agaorse gel (of appropriate concentration) containing no fluorescent dye. The stain contained in the ladder replaces ethidium bromide, thus eliminating its addition to the gel.</p>

<p>These Ready-to-Load ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis. The concentration of each band is kept uniform to give sharp bands. The ladder consists of 12 linear double-stranded fragments. A single or multiple bands in the ladder are made intense to orient the user to the other bands in the ladder. The 1,000bp fragment is present at increased intensity to allow easy identification. Contains Bromophenol Blue and Xylene Cyanol as the tracking dyes.<br> Size range : 200 to 4000 bpMix concentration : 108 ng /µlRecommended loading volume is 9 µl (1µg) </p>

<p>Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.</p>

<p>HSP Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. HSP Taq DNA Polymerase can amplify DNA target up to 5 kb. The elongation velocity is 0.9~1.2kb/min. It has 5' to 3' polymerase activity but lacks 3' to 5' exonuclease activity that results in a 3'-dA overhang PCR product. All components of the HSP PCR Buffer are at optimal concentration for efficient amplification. It contributes to highly specific incorporation of primer and template.</p>

<p>These ready -to-Load DNA ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis.<br> The concentration of each band is kept uniform to give sharp bands.<br> A single or multiple bands in the ladder are made intense to orient the user to the other bands in the ladder. In this ladder the 500bp and 1200bp fragments are present at increased intensity to allow easy identification.<br> Contains Bromophenol Blue and Xylene Cyanol as the tracking dyes.<br> Size range : 100 to 3000 bp<br> Mix concentration : 136 ng /µl<br> Recommended loading volume is 7 µl (1 µg) </p>

<p>These Ready-to-Load ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis. The concentration of each band is kept uniform to give sharp bands. The ladder consists of 13 linear double-stranded fragments. A single or multiple bands in the ladder are made intense to orient the user to the other bands in the ladder. The 250bp and 500bp fragments are present at increased intensity to allow easy identification. Contains Bromophenol Blue as the tracking dyes.Size range : 50 to 1000 bpMix concentration :128 ng /µlRecommended loading volume is 8 µl (1µg) </p>

<p>Taq Plus, a mixture of Taq and Pfu polymerase, blends the processivity of Taq with the high fidelity of Pfu. Therefore, this specially formulated Taq plus allow amplification of the higher fidelity and longer templates than the single-enzyme formulations. It is also a better choice for amplifying complex template, such as GC-rich template. And it is suitable as a direct replacement for ordinary Taq Polymerase in most applications. In addition, Using Taq plus results in 3´-dA overhangs PCR products, which can be used in TA clone.</p>

<p>This is a DNA polymerase coated with an antibody, which is suitable for hot start PCR. There is no activity of the enzyme at room temperature, and the antibody can be inactivated rapidly at 70oC or above. In the pre-denaturation step of PCR program, the antibody is completely inactivated. An initial activation step at 95oC is required for activation of the protein. This step denatures antibodies linked to the active center of the enzyme. When the specific antibodies detach from Taq-polymerase, the amplification proceeds with greater specificity<br> Hot start PCR significantly reduces nonspecific priming, the formation of primer dimers, and often, increases product yields. It offers convenient room temperature reaction setups.<br> Inhibits Taq polymerase activity at low temperatures. Gets activated on reaching higher temperatures and the initial denaturation step. </p>

<p>The FluroBronze 100bp ladder is specially prepared as ready-to-load fluorescent ladders and are ideal for determining the size of double-stranded DNA from 100 to 1500 base pairs. The ladder consists of 11 linear double-stranded fragments .The 500bp fragment is present at increased intensity to allow easy identification. The flurobronze ladder contains in addition to the tracking dye, a non-carcinogenic nucleic acid stain.The new range of DNA ladders are specially prepared as ready-to-load fluorescent ladders. The flurobronze ladder contains in addition to the tracking dye, a non-carcinogenic nucleic acid stain. The ladder should be loaded onto an agaorse gel (of appropriate concentration) containing no fluorescent dye.</p>

<p>These Ready-to-Load ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis.<br> The concentration of each band is kept uniform to give sharp bands.A single or multiple bands in the ladder are made intense to orient the user to the other bands in the ladder.<br> Mix Concentration: 100ng/µl<br> Recommended loading volume is 10µl.Size range : 100 to 1500 bp Contains Bromophenol Blue and Xylene Cyanol as the tracking dyes </p>

<p>Plus Mix (2X) is a premixed, ready-to-use solution containing Taq Plus DNA Polymerase, dNTP, Mg2+ and Reaction Buffer a optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Plus Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding enhancer.<br> Taq Plus, a mixture of taq and pfu polymerase, blends the processivity of taq with the high fidelity of pfu. Therefore, this specially formulated Taq plus allow amplification of the higer fidelity and longer templates<br> than the single-enzyme formulations. It is also a better choice for amplifying complex template, such as GC-rich template. And it is suitable as a direct replacement for ordinary Taq Polymerase inmost applications. In addition, Using Taq plus results in 3´-dA overhangs PCR products, which can be used in TA clone </p>

<p>HSP 2X Mix is a premixed, ready-to-use solution containing HSP Taq DNA Polymerase, dNTP, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. HSP Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher specificity by optimizing the system, reducing primer-dimer rate.<br> HS Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. HS Taq DNA Polymerase can amplify DNA target up to 5 kb. The elongation velocity is 0.9~1.2kb/min. It has 5' to 3' polymerase activity but lacks 3' to 5' exonuclease activity that results in a 3'-dA overhang PCR product. All components of the HSP Mix are at optimal concentration for efficient amplification. It contributes to highly specific incorporation of primer and template. </p>

<p>2X Taq Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTP, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. The mix retains all features of Taq DNA Polymerase.</p>

<p>2X Roseate PCR Master Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTP, Mg2+ , Reaction Buffer and Tracking dye at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. The mix retains all features of Taq DNA Polymerase.<br> This Master Mix has a roseate coloured dye enabling the researcher to load the sample directly after PCR onto an agarose gel for electrophoresis. </p>

<p>2X FSP Taq Mix is a premixed, ready-to-use solution containing FSP Taq DNA Polymerase, dNTP, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. <br> The mix retains all features of FSP Taq DNA Polymerase - possesses high amplification efficiency and fast elongation. Can be used in various kinds of PCR. It has an elongation rate 2x higher than regular Taq DNA polymerase, and can shorten the amplification time by half. It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity, which results in a 3'-dA overhangs PCR product. </p>

Pfu Mix(2x) is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTP, MgSO4 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Pfu Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding intensifier and optimizer. Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.

<p>FSP Taq Mix Direct for Blood (2X) is a premixed, ready-to-use solution containing FSP Taq DNA Polymerase, dNTP mix, and all other PCR components, except DNA template and primers. FSP Taq Mix Direct for Blood is specific for whole blood amplification. It contributes to fast, specific, sensitive and reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. The FSP Mix (2X) can be used with conventional PCR machines.</p>