Outros

<p>Chromogenic substrate for β-D-glucosidase. Yields a red precipitate upon cleavage.</p>

<p>5-Bromo-6-chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-D-galactosidase, yielding a magenta precipitate and contained in a chromogenic culture media. It is an indolyl carbohydrate that is the alpha-D-mannoside of indoxyl, in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively.</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>Chromogenic cephalosporin substrate for the detection of beta-lactamase activity</p>

<p>4-Nitrophenyl-beta-L-fucopyranoside is a high-quality pNP enzyme substrate developed for the sensitive detection and quantification of Beta-L-fucosidase activity. The enzymatic hydrolysis of the substrate generates 4-nitrophenol, a yellow chromogenic component that can be conveniently measured spectrophotometrically, allowing for accurate determination of enzyme concentration and kinetics. This substrate finds applications in various research fields, diagnostics, and industrial enzyme production.</p>

<p>Phosphoric acid, mono(4-nitrophenyl) ester is an inhibitor that binds to the human serum albumin (HSA), thereby preventing it from binding to the transcriptional regulatory protein. This leads to a decrease in the activity of this protein and consequently less inhibition of gene expression. The structural analysis of this inhibitor has shown that it forms hydrogen bonding interactions with basic proteins, while its reactivity is mainly dependent on Michaelis–Menten kinetics. The inhibitor also inhibits enzyme activities by binding to their active sites, reducing the enzyme's ability to catalyze reactions.</p>

<p>A chromogenic substrate for β-galactosidase.</p>

<p>Bronze-Gal (Bronze-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a bronze colored precipitate. Bronze-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.</p>

<p>5-Bromo-4-chloro-3-indolyl nonanoate is a fluorogenic substrate that is used in the detection of enzymes. The substrate reacts with various enzymes and produces a fluorescent product, which can be detected using a fluorimeter or luminometer. It has been shown to be active against β-galactosidase, α-chymotrypsin, and β-glucuronidase. This compound may also be used as an enzyme substrate for the detection of bacteria in culture media or for food testing. 5-Bromo-4-chloro-3 indolyl nonanoate is not mutagenic or genotoxic when tested on bacterial cells.</p>

<p>2-Nitrophenyl b-D-cellobioside heptaacetate is a chromogenic enzyme substrate used to assay for cellobiohydrolase activity. The substrate undergoes enzymatic hydrolysis, releasing 2-nitrophenol which can then be detected via absorbance at 400 nm. This type of substrate is also used for the analysis of cellulose and hemicelluloses in plant materials. The heptaacetate modification enhances solubility, making it ideal for use in organic solvents, and it also protects against unwanted hydrolysis by acid or alkali. The substrate can be used in a variety of applications, including enzyme assay development, screening for inhibitors or activators, and in the study of the mode of action and substrate specificity of cellulase enzymes.</p>

<p>2-Nitrophenyl-beta-D-galactopyranoside is a non-animal origin chromogenic substrate for the detection of beta-galactosidase activity. It is used in food testing, diagnostics, and environmental testing. 2-Nitrophenyl-beta-D-galactopyranoside is also used as a ligand in enzyme assays and a fluorogenic substrate for chemiluminescent reactions. This product is conjugated to HRP.</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>5-Bromo-6-chloro-3-indoxylphosphate, disodium salt trihydrate is a bioluminescent substrate that can be used in a variety of diagnostic applications. This compound reacts with luciferase to yield light and can serve as an indicator for various enzymes and chemical reactions. 5-Bromo-6-chloro-3-indoxylphosphate, disodium salt trihydrate has been shown to be compatible with both culture media and food testing.</p>

<p>N-Acetylmemantine is a drug that inhibits the activity of acetylcholinesterase in the brain. It has been shown to inhibit cancer cell growth and induce apoptosis, as well as inhibit the reaction of hydrolysis of acetonitrile with hydrochloric acid in an organic solvent. N-Acetylmemantine is synthesized by heating ethylene in the presence of hydrochloric acid. The synthesis yields a white solid that consists mainly of N-acetylmethamphetamine. This drug interacts with cellular membranes, which may be due to its ability to react with cholesterol or phospholipids. The interaction leads to changes in membrane permeability, which can result in apoptosis.</p>

<p>Aldol® 518 alpha-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of alpha-galactosidase activity. The enzyme substrate is cleaved by alpha-galactosidase positive bacteria and yeasts in liquid or solid media, yielding red color and red fluorescence signal. Aldol® 518 alpha-D-galactopyranoside can also be used to detect alpha-galactosidase (encoded by the Mel1 gene) in yeast gene reporter assays or for identification of alpha-gal positive transformants on agar plates. Aldol® 518 alpha-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>6-Bromo-2-naphthyl beta-D-glucuronide is a substrate for beta-glucuronidase, an enzyme involved in detoxification and bacterial metabolism. Upon enzymatic hydrolysis, it releases 6-bromo-2-naphthol, which forms a detectable dye when reacted with diazonium salts. This compound is widely used in microbiology to detecting E. coli in water testing and histochemical staining. 6-Bromo-2-naphthyl beta-D-glucuronide is also been used in cancer research studies. It has been found that some tumors are related to a high activity of beta-glucuronidase, responsible to reactivate the carcinogens and promote tumor progression, developing a drug resistance to chemotherapy.</p>

<p>Chromogenic substrate for esterase with C9 activity yielding a salmon colored precipitate upon cleavage.</p>

<p>Chromogenic substrate for cellobiohydrolase and endoglucanase.</p>

<p>2-Chloro-4-nitrophenyl b-D-maltoside is a fluorescent substrate used in diagnostic kits. It is used to detect the presence of beta-glucuronidase enzymes, which are found in many bacteria and fungi. 2-Chloro-4-nitrophenyl b-D-maltoside has been shown to be a superior substrate for beta-glucuronidase as it can be used at lower concentrations and is more stable than other substrates. The enzyme hydrolyzes the 2,4 nitrophenyl ester bond and releases the fluorogenic product 4-(2'-hydroxyethyl)benzene diazonium chloride (HED). This product emits light under ultraviolet light, making it ideal for use in Bioluminescence Assays.</p>

<p>Chromogenic beta-Glucosidase substrate yielding a yellow solution upon cleavage.</p>

<p>2-Naphthyl-lpha-D-glucopyranoside is a substrate for α-glucosidase. 2-Naphthol is released upon hydrolyzation. By simultaneous coupling with a suitable staining reagent, such as hexazonium p-rosaniline, the corresponding reddish-brown azo-dye is formed. Naphthols can also be detected by fluorescence analysis.</p>

<p>3-Indoxyl sulfate, potassium salt (3ISK) is a chemical compound that is soluble in water. It has been used as a marker for bowel disease, and it has been shown to react with analytical methods such as titration calorimetry and lc-ms/ms. 3ISK can be used to determine the optimum concentration for pharmacological treatment of bowel disease. The metabolic fate of 3ISK has been studied extensively and it is found that 3ISK is metabolized by microbial enzymes in the gut, which may lead to an increased incidence of bowel disease in patients with low levels of microbial metabolism. 3ISK also plays an important physiological function by maintaining mitochondrial membrane potential, which could have implications for pharmacological treatment of bowel disease.</p>

<p>2-(N-Hexadecanoylamino)-4-nitrophenylphosphocholine hydroxide, often abbreviated as HDA-PC, is a chromogenic substrate for acid sphingomyelinase enzyme. HDA-PC is used as a key substrate for measuring the activity of this enzyme, responsible for catalyzing the hydrolysis of sphingomyelin to ceramide. The activity assay for this measuring this enzyme's acitivy is to detect a deficiency (or lack) of the enzyme which is directly related to the Niemann-Pick disease.</p>

<p>4-Nitrophenyl-N-acetyl-beta-D-galactosaminide is a synthetic substrate that is used in enzyme kinetics. It is a beta-galactosidase substrate that reacts with the enzyme to form a product. The rate of reaction can be measured by following the change in absorbance over time. This product will react with an acceptor, such as chondroitin sulfate, to produce an observable reaction product. 4NPBGA has been shown to be an effective carbon source for plant physiology studies and has been shown to have optimal activity at pH 6.5.</p>

<p>4-Nitrophenyl beta-D-cellotrioside is a chromogenic substrate for cellulase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl beta-D-cellotrioside is used in different applications such as the Biomass conversion research, paper industry applications</p>

<p>5-Bromo-4-chloro-1-methyl-3-indolyl-β-D-glucopyranoside is a fluorescent substrate used for the detection of β-glucuronidase. It can be used in several enzyme substrates, such as 5,5'-dithiobis(2-nitrobenzoic acid), 5,5'-dithiobis(3,4,5,-trimethoxybenzoic acid), and 4-(p-[N-(7-(2,7'-dimethyl -2H -chromenyl)-2H -indazolium)]phenylazo)benzoic acid. This product is used for diagnostics and culture media as well as food testing. The product has an extremely high purity and a CAS number.</p>

<p>4-Nitrophenyl a-D-thiomannopyranoside is a chemiluminescence substrate that can be used to measure the activity of β-galactosidase, an enzyme involved in the hydrolysis of lactose. This product is available as a white to yellowish powder and has been shown to have high purity and quality. It also displays strong fluorescence when reacted with hydrogen peroxide and has been used for diagnostics tests such as staining, food testing, and enzyme substrate reactions.</p>

<p>Aldol® 470 nonanoate is a chemiluminescent substrate that can be used as a ligand for the detection of C8/C9-esterases, lipases activity, to result in an orange/green color. Aldol® 470 nonanoate has been shown to be active with horseradish peroxidase in a number of different assay systems. The purity and quality of Aldol® 470 nonanoate has been tested with culture media, environmental testing, bioluminescence, staining, fluorogenic substrate, chromogenic substrate, enzyme substrate, food testing and conjugates.</p>

<p>5-Bromo-6-chloro-3-indoxyl butyrate is an enzyme substrate that is used for the detection of bacteria and fungi in environmental testing, food testing, and diagnostics. It is also used as a fluorogenic substrate in bioluminescence and chemiluminescence assays. 5-Bromo-6-chloro-3-indoxyl butyrate binds to proteins on the cell surface, causing an increase in fluorescence when excited by light of a specific wavelength. The conjugate can be detected using a variety of methods, including staining with dyes such as safranin or methylene blue. This product has high purity and high quality.</p>

<p>Aldol 515 caprylate hydrochloride is a high-quality fluorogenic substrate that is used for the detection of enzymes in culture media and enzyme substrates. The product has a CAS number of 2484873-14-3 and can be used for food testing, diagnostics, and environmental testing. Aldol 515 caprylate hydrochloride can also be used as a ligand or chromogenic substrate to measure bioluminescence.</p>

<p>Substrate for the detection of beta-D-fucosidase</p>

<p>2-Nitrophenyl-1-thio-beta-D-galactopyranoside is a nitroaryl compound that can be synthesized from beta-D-galactopyranosides and nitric acid. It has a molecular conformation that is stabilized by hydrogen bonds, which allows it to form supramolecular assemblies with other molecules. The nitro group in this molecule is responsible for the formation of the nitro group in 1,2,4,5,-tetranitrobenzene.</p>

<p>5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside (X-Fuc) is a chromogenic substrate specifically designed for the identification and detection of fucosidase enzyme activity. Upon cleavage by fucosidase, it generates a blue-colored 5-Bromo-4-chloro-3-hydroxy-indole precipitate, which can be easily observed under a microscope or spectrophotometer. This feature makes X-Fuc an invaluable tool across various applications, such as biochemical analysis, histochemical staining, cell biology, and enzyme kinetic assays. By providing a fast, sensitive, and reliable readout for fucosidase activity, 5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside facilitates essential research in the understanding of biological processes and the development of therapeutic interventions.</p>

<p>6-Bromo-2-naphthyl-b-D-xylopyranoside (BNX) is a chromogenic enzyme substrate used primarily to detect xylosidase activity. It is cleaved by the enzyme, resulting in the release of a colored product that can be measured spectrophotometrically. BNX is widely used as a substrate in research studies for the detection and quantification of various glycoside hydrolases, and it is particularly useful for studying cellulases and xylanases.</p>

<p>Chromogenic α-mannosidase substrate yielding a absorbent solution upon cleavage. Also used for studies of the crystal structure of the complexes of concanavalin A and binding studies with concanavalin A.</p>

<p>Aldol® 495 inositol-phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidylinositol specific phospholipase C activity (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC or PI-PLC-positive microorganisms in liquid or solid media, yielding orange color. In addition, green fluorescence is generated when a suitable matrix is present, e.g. PE-Pellets or cellulose acetate filter. Aldol® 495 inositol-phosphate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chemiluminescent substrate that can be used in the detection of various enzymes. This product has been tested for sterility and purity, and it has passed all tests. It is also free of any detectable levels of heavy metals or other contaminants. 4NP2AG6S is a high quality bioluminescent substrate with CAS No. 71498-92-5. It is a fluorescent compound that reacts with an enzyme to produce light. The reaction can be detected by measuring the emitted light intensity at 680 nm (max).</p>

<p>Aldol® 518 beta-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-glucosidase activity. The colorless enzyme substrate is cleaved by beta-glucosidase-producing bacteria in liquid or solid media, yielding intense red color and red fluorescence. Aldol® 518 beta-D-glucopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>Aldol® 518 choline phosphate is a fluorogenic substrate that is used in the detection of enzyme activity. It has been shown to be an effective substrate for enzymes such as beta-galactosidase and phosphatases, and can be used in the detection of bacterial growth. Aldol® 518 choline phosphate is also a chromogenic substrate for the detection of alkaline phosphatase. It has been shown to have high purity and bioluminescence properties, making it useful for detecting bacteria in culture media or environmental testing. The conjugate of Aldol® 518 choline phosphate with various ligands can be used to detect specific proteins or antibodies.</p>

<p>4-Nitrophenyl-alpha-D-glucopyranoside, beta-Anomer is a chromogenic sugar that can be used as an indicator for the presence of 4-nitrophenol. It is a highly reactive compound that spontaneously reacts with nitrobenzene to form a dinitroaniline. This anomeric structure is easily oxidized by air and light to form the corresponding o-dinitroaniline. When this reaction occurs, this substance changes color from yellow to red.</p>

<p>Chromogenic substrate for β-D-fucosidase yielding a blue precipitate.</p>

<p>Chromogenic substrate to visualize beta-glucuronidase activity; produces a red color</p>

<p>4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-b-D-thioglucopyranoside is a fluorescent dye that is used in recording and registration. It has been shown to be a very sensitive reporter of protein interactions in living cells. This dye binds to proteins via the hydroxyl groups on its sugar moiety, and has been shown to bind to a variety of proteins such as myosin light chain kinase and phosphatidylinositol 4-kinase. The fluorescent properties of this dye make it useful for tracking protein localization in the cell.</p>

<p>Aldol® 495 beta-D-glucuronic acid is a chromogenic and fluorogenic enzyme substrate used for detection of beta-glucuronidase activity. The living cells of Escherichia coli can be reliably detected in growth media containing Aldol® 495 beta-D-glucuronic acid, as beta-glucuronidase occurs almost exclusively in E. coli. Aldol® 495 beta-D-glucuronic acid can also be in GUS reporter assays. Aldol® 495 beta-D-glucuronic acid can be used in liquid culture and solid media, and is suitable for use under aerobic and anaerobic conditions. Upon exposure to active beta-glucuronidase, Aldol® 495 beta-D-glucuronic acid develops orange colour and green fluorescence.</p>

<p>N-Methylindoxyl-beta-D-galactopyranoside monohydrate (MG) is a chromogenic probe that interacts with galactose residues found on glycoproteins, which are found on cell surfaces, leading to activation of cells. MG also binds specifically to the surface of lung cells and can inhibit the development of certain types of infections..</p>

<p>Naphthol AS-BI-beta-D-glucuronic acid is a β-glucuronidase substrate. The substrate is used in combination with stabilized diazonium salts such as Fast Red Violet LB salt (5-chloro-4-benzamido-2-methylbenzenediazonium chloride). Free Naphthol AS and Fast Red Violet undergo a chemical reaction to yield intensely colored azo-dyes.</p>

<p>8-Hydroxyquinoline b-D-glucopyranoside (8-OHG-glu) is a chromogenic enzyme substrate commonly used to detect the presence of beta-glucosidase in biological samples. When hydrolyzed by beta-glucosidase, 8-OHG-glu produces a yellow-orange color, indicating the presence of the enzyme. This substrate is particularly useful for measuring beta-glucosidase activity in soil and plant samples.</p>

<p>4-Nitrophenyl beta-rutinoside is a chromogenic substrate for beta-glucosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl beta-rutinoside is used in different applications such as the Flavonoid glycoside hydrolysis, food industry applications</p>

<p>Chromogenic β-glucosaminidase substrate yielding a yellow solution upon cleavage, used particularly in yeasts and molds.</p>

<p>5-Bromo-4-chloro-3-indoxyl-alpha-D-fucopyranoside is a photosynthetic agent that belongs to the class of fucopyranosides. It is a non-proteinogenic amino acid that has been shown to be encoded in chloroplasts and mitochondria in photosynthetic organisms. 5B4C3IF has also been found to be involved in the synthesis of polynucleotides and polypeptides, as well as the regulation of biomass.</p>