Outros

<p>4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-b-D-thioglucopyranoside is a fluorescent dye that is used in recording and registration. It has been shown to be a very sensitive reporter of protein interactions in living cells. This dye binds to proteins via the hydroxyl groups on its sugar moiety, and has been shown to bind to a variety of proteins such as myosin light chain kinase and phosphatidylinositol 4-kinase. The fluorescent properties of this dye make it useful for tracking protein localization in the cell.</p>

<p>4-Nitrophenyl-alpha-D-glucopyranoside, beta-Anomer is a chromogenic sugar that can be used as an indicator for the presence of 4-nitrophenol. It is a highly reactive compound that spontaneously reacts with nitrobenzene to form a dinitroaniline. This anomeric structure is easily oxidized by air and light to form the corresponding o-dinitroaniline. When this reaction occurs, this substance changes color from yellow to red.</p>

<p>Chromogenic β-mannosidase substrate yielding a yellow solution upon cleavage.</p>

<p>The maunfacturing of blue jeans started 150 years ago as workwear, and are found nowadays in almost every wardrobe. Their renowned indigo color tone is truly unique; nevertheless, the high demand for the dye poses serious sustainability and environmental problems connected with its manufacturing process. Recently, a group of scientists presented an alternative indigo dyeing method inspired by the indoxyl glucoconjugate present in the plant P.tinctorium. The researchers proposed as an alternative approach a biocatalytic process using recombinant E.coli to access water soluble β-gluco-indoxyl (indican). At the point of coloring, indican gets hydrolyzed by β-glucosidase enzymes forming indoxyl, which suddenly undergoes oxidation to leucoindigo and further crystallizes to indigo giving the typical blue denim its signature character.</p>

<p>5-Bromo-4-chloro-3-indolyl N-acetyl-a-D-neuraminic acid ammonium salt is a substrate used to detect and quantify sialidase activity. Sialidases are enzymes that cleave sialic acid residues from glycoconjugates, and their activity is important in various cellular processes. The substrate is commonly used in research aimed at understanding the role of sialidases in diseases such as influenza and cancer.</p>

<p>2-Naphthyl beta-D-galactopyranoside is a glycoside that is found in the flowers of the plant Eschscholzia californica. It is used as a localizer for bacterial strains and can be hydrolyzed by enzymes to release naphthalene. 2-Naphthyl beta-D-galactopyranoside has been shown to inhibit the growth of corynebacterium and diazonium salt, which may be due to its ability to bind to DNA and disrupt protein synthesis. The potential use of this molecule is for its insolubility, which prevents it from being absorbed by skin cells.</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-4-O-(b-D-galactopyranosyl)-b-D-glucopyranoside is an efficient chromogenic pNP enzyme substrate ideal for monitoring enzyme activity and understanding carbohydrate metabolism. This galacto-glucoside compound is designed specifically for use with glycosidases such as hexosaminidases and delivers unparalleled performance in your enzymatic assays.</p>

<p>Aldol 515 caprylate hydrochloride is a high-quality fluorogenic substrate that is used for the detection of enzymes in culture media and enzyme substrates. The product has a CAS number of 2484873-14-3 and can be used for food testing, diagnostics, and environmental testing. Aldol 515 caprylate hydrochloride can also be used as a ligand or chromogenic substrate to measure bioluminescence.</p>

<p>4-Nitrophenyl N,N'-diacetyl-beta-D-chitobioside is a chromogenic substrate for chitinase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl N,N'-diacetyl-beta-D-chitobioside is used in different applications such as the Fungal infection detection, chitin degradation assays</p>

<p>Histochemical substrate for alkaline phosphatase</p>

<p>2-(N-Hexadecanoylamino)-4-nitrophenylphosphocholine hydroxide, often abbreviated as HDA-PC, is a chromogenic substrate for acid sphingomyelinase enzyme. HDA-PC is used as a key substrate for measuring the activity of this enzyme, responsible for catalyzing the hydrolysis of sphingomyelin to ceramide. The activity assay for this measuring this enzyme's acitivy is to detect a deficiency (or lack) of the enzyme which is directly related to the Niemann-Pick disease.</p>

<p>4-Chloro-3-indoxyl-1,3-diacetate is a fluorogenic or chromogenic substrate used in food testing. It is also an enzyme substrate that can be used to detect the presence of bioluminescence. 4-Chloro-3-indoxyl-1,3-diacetate has been shown to have high purity and high quality for use in diagnostic kits. 4-Chloro-3-indoxyl-1,3-diacetate is a ligand that is conjugated with other molecules to form complexes for use in fluorescence and chemiluminescence experiments.</p>

<p>Naphthol AS-BI N-acetyl-beta-D-glucosaminide is a fluorogenic substrate for the pararosaniline-glyoxalase (PG) reaction. This substrate is used to study enzymatic activity in two human isoenzymes of PG, PG I and PG II. The conversion of this substrate by PG I or PG II results in a color change from yellow to red. Naphthol AS-BI N-acetyl-beta-D-glucosaminide may be used as an experimental tool to measure the activity of these enzymes and to study the effect of inhibitors on their activity. It can also be used as a marker for studying enzyme transfer between cells.</p>

<p>5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside (X-Fuc) is a chromogenic substrate specifically designed for the identification and detection of fucosidase enzyme activity. Upon cleavage by fucosidase, it generates a blue-colored 5-Bromo-4-chloro-3-hydroxy-indole precipitate, which can be easily observed under a microscope or spectrophotometer. This feature makes X-Fuc an invaluable tool across various applications, such as biochemical analysis, histochemical staining, cell biology, and enzyme kinetic assays. By providing a fast, sensitive, and reliable readout for fucosidase activity, 5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside facilitates essential research in the understanding of biological processes and the development of therapeutic interventions.</p>

<p>Chromogenic substrate for cellobiohydrolase and endoglucanase.</p>

<p>6-Bromo-2-naphthyl beta-D-glucuronide is a substrate for beta-glucuronidase, an enzyme involved in detoxification and bacterial metabolism. Upon enzymatic hydrolysis, it releases 6-bromo-2-naphthol, which forms a detectable dye when reacted with diazonium salts. This compound is widely used in microbiology to detecting E. coli in water testing and histochemical staining. 6-Bromo-2-naphthyl beta-D-glucuronide is also been used in cancer research studies. It has been found that some tumors are related to a high activity of beta-glucuronidase, responsible to reactivate the carcinogens and promote tumor progression, developing a drug resistance to chemotherapy.</p>

<p>Chromogenic substrate for α-amylase yielding a yellow solution upon cleavage.</p>

<p>Aldol® 470 choline phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-choline specific phospholipase C (PC-PLC, EC 3.1.4.3) activity. The colorless product is cleaved by PC-PLC positive microorganisms in liquid or solid media, yielding a yellow color and green fluorescence. Aldol® 470 choline phosphate therefore, enables the detection and identification of PC-PLC positive pathogenic bacteria, such as, Clostridium perfringens, Pseudomonas aeruginosa, Helicobacter pylori, Legionella pneumophila, Bacillus cereus, Bacillus anthracis, Listeria monocytogenes, and others. Aldol® 470 choline phosphate can be used under aerobic and anaerobic conditions.</p>

<p>Chromogenic substrate for N-acetyl-β-D-glucosaminidase yielding a blue precipitate. Contained in certain mycological culture media. Used in histochemistry.</p>

<p>5-Bromo-4-chloro-3-indolyl b-D-fucopyranoside is a chromogenic substrate used is for the enzyme beta-fucosidase.</p>

<p>5-Bromo-4-chloro-3-indoxyl-beta-D-xylopyranoside is a fluorogenic substrate that can be used to measure the activity of bacterial esterases. The enzyme cleaves the xylose moiety from 5-bromo-4-chloro-3-indoxyl, yielding indoxyl as an intermediate product. This reaction can be catalyzed by esterases found in bacteria, which hydrolyze esters containing a single hydroxyl group at one end and a carboxylic acid or alcohol at the other end. Indoxyl is fluorescent and can be detected using a fluorimeter. 5BX is also chromogenic, so it can be used as an enzyme substrate for detecting beta galactosidase activity in bacteria such as Mycobacterium smegmatis or Escherichia coli.<br>5BX is also used to detect chemilumines</p>

<p>2-Chloro-4-nitrophenyl-beta-D-galactopyranoside is a chromogenic substrate used to test enzyme activities. It is commonly used in molecular biology research for the detection and measurement of beta-galactosidase activity.</p>

<p>Chromogenic substrate for β-D-galactosidase, yielding a blue precipitate upon cleavage.</p>

<p>4-Nitrophenyl palmitate is a lipase-sensitive chromogenic substrate commonly used in the study of lipase and esterase activity. Its hydrolysis by lipases produces a yellow 4-nitrophenol, which can be easily detected spectrophotometrically, offering a quantitative measure of enzyme activity. This substrate is widely utilized in the screening of lipase-producing microorganisms and the optimization of enzyme-catalyzed reactions in various fields, including biotechnology and pharmaceutical research.</p>

<p>Chromogenic substrate for β-D-glucuronidase (GUS) yielding a magenta precipitate. It is used fordetection of GUS+ bacterial colonies as analternative to 5-bromo-4-chloro-3-indoxyl-β-D-glucuronide (X-GlcA).</p>

<p>2-Chloro-4-nitrophenyl alpha-D-mannopyranoside is a substrate used to measure the alpha-mannosidase activity. This enzyme plays a crucial role in the lysosomal degradation of asparagine-linked carbohydrate cores of glycoproteinsis. The use of 2-Chloro-4-nitrophenyl alpha-D-mannopyranoside in diagnostic assays is important as a deficiency in alpha-mannosidase leads to the accumulation of mannose-rich oligosaccharides in lysosomes, causing a range of health problems collectively known as alpha-mannosidosis.</p>

<p>5-Bromo-4-chloro-3-indolyl nonanoate is a fluorogenic substrate that is used in the detection of enzymes. The substrate reacts with various enzymes and produces a fluorescent product, which can be detected using a fluorimeter or luminometer. It has been shown to be active against β-galactosidase, α-chymotrypsin, and β-glucuronidase. This compound may also be used as an enzyme substrate for the detection of bacteria in culture media or for food testing. 5-Bromo-4-chloro-3 indolyl nonanoate is not mutagenic or genotoxic when tested on bacterial cells.</p>

<p>Aldol® 518 myo-inositol phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidylinositol specific phospholipase C (PI-PLC, EC 4.6.1.13) activity. The colorless enzyme substrate is cleaved by PI-PLC positive microorganisms grown in liquid or solid media, yielding intense red color. In combination with Aldol® 355 fluorescence enhancer, red fluorescence can also be obtained. Aldol® 518 myo-inositol phosphate thus enables the detection and identification of various bacteria, such as, pathogenic strains of Listeria, Clostridium, Bacillus, and Staphylococcus species. Aldol® 518 myo-inositol phosphate is suitable for use under aerobic and anaerobic conditions.</p>

<p>Chromogenic substrate for esterase with C8 activity yielding a blue precipitate upon cleavage.</p>

<p>Bronze-Gal (Bronze-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a bronze colored precipitate. Bronze-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.</p>

<p>2-Nitrophenyl b-D-arabinopyranoside is a chemical compound that is used in the detection of ATP by chemiluminescence. It acts as a substrate for enzymes such as luciferase and catalase, which catalyze the oxidation of 2-nitrophenyl b-D-arabinopyranoside to phenol and carbon dioxide with the release of light. This reaction can be used in diagnostic applications such as detecting bacteria in culture media or diagnosing food spoilage. 2-Nitrophenyl b-D-arabinopyranoside can also be used as a conjugate to detect proteins or nucleic acids by fluorescence spectroscopy. The CAS number for this compound is 107772-06-1.</p>

<p>2,4-Dinitrophenyl-beta-D-glucopyranoside is a chromogenic substrate used to assay enzymes, such as glucosidases. These enzymes catalyze the hydrolysis of glycosidic bonds in sugars.</p>

<p>Chromogenic substrate for α-mannosidase yielding a salmon colored precipitate upon cleavage.</p>

<p>6-Bromo-2-naphthyl-alpha-D-galactopyranoside is a bioluminescent substrate for the enzyme luciferase that has been shown to be useful in detection of phenazines, antibiotics and other compounds in environmental testing. 6-Bromo-2-naphthyl-alpha-D-galactopyranoside can also be used for measurements of total microbial counts and for the measurement of the activity of enzymes such as beta galactosidase. It is also a ligand for the peroxidase enzyme, which makes it useful in staining techniques. 6BARG can be used as a fluorogenic substrate or as an indicator in chromogenic substrates. It is an important reagent for chemiluminescence and can also be used to measure the concentration of various substances.</p>

<p>Chromogenic substrate for beta-glucanase</p>

<p>Chromogenic substrate for beta-glucuronidase</p>

<p>Substrate for determining hydrolytic activity of chitinase</p>

<p>Aldol 458 acetate is a synthetic chemical that is used in the food industry as an enzyme substrate and in diagnostic tests to measure the level of enzymes. Aldol 458 acetate is a ligand that binds to proteins, such as horseradish peroxidase and alkaline phosphatase, which are commonly used for detection of antibodies or other proteins. This product can be used as a culture medium additive for bacteria, fungi, and yeast. It can also be used for diagnostics and chemiluminescence reactions. Aldol 458 acetate has high purity and quality.</p>

<p>Substrate for beta-galactosidase</p>

<p>3-Indoxyl phosphate, p-toluidine salt is a fluorogenic substrate that can be used for the detection of β-galactosidase. 3-Indoxyl phosphate, p-toluidine salt is a highly active chromogenic substrate that can be used as a diagnostic tool in the food industry to determine whether or not milk has been adulterated with animal milk. This product is also used in environmental testing to detect bacteria and fungi. 3-Indoxyl phosphate, p-toluidine salt is a high purity, high quality conjugate that can be used for the detection of β-galactosidase.</p>

<p>Aldol® 495 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless enzyme substrate is cleaved by lactose-utilizing bacteria in liquid or solid media, yielding orange color as well as green fluorescence if a fluorescence enhancer or a suitable matrix are present. Aldol® 495 beta-D-galactopyranoside can also be used in beta-gal reporter gene assays or for identification of beta-gal positive transformants on agar plates. Aldol® 495 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>Aldol® 515 propionate is a chromogenic substrate for many enzymes, including beta-galactosidase, β-glucuronidase, α-galactosidase, β-galactosidase, and acid phosphatase. It can be used as a substrate in the detection of staining and enzyme activity in diagnostic testing. Aldol® 515 propionate has been shown to be an effective ligand for the protein Glu-C.</p>

<p>6-Chloro-3-indolyl b-D-glucuronide sodium salt is a chromogenic substrate for beta-glucuronidase, a reporter gene used to monitor gene expression by detecting the enzyme's activity. It yields a salmon colored precipitate upon cleavage.</p>

<p>Chromogenic substrate for carboxylesterase yielding a magenta precipitate upon cleavage. Mainly used in histochemistry.</p>

<p>6-Bromo-2-naphthyl 2-acetamido-2-deoxy-beta-D-glucopyranoside is a chromogenic substrate used for the detection of chitinase activity. Upon hydrolysis by chitinase, it releases a yellow-brown color, which is easily detected by spectrophotometry. This substrate has been used to study chitinases in various organisms, including bacteria, fungi, and insects. It can also be used to quantitate chitinase activity in various biological samples.</p>

<p>2-O-(4-Nitrophenyl)-a-D-N-acetylneuraminic acid ammonium salt is a chromogenic substrate commonly used in the detection and quantification of sialidase activity. Sialidases are a family of enzymes that hydrolyze sialic acid residues from glycoconjugates. This substrate is used to detect bacterial and viral sialidases, which play an important role in pathogen infectivity. The ammonium salt form of the substrate is preferred for its solubility in water-based assay systems.</p>

<p>Chromogenic substrate for fucosyltransferases</p>

<p>Chromogenic β-Glucosaminidase substrate yielding a yellow absorbent solution upon cleavage.</p>

<p>Aldol 535 is a chemiluminescent substrate for the detection of myo-inositol-1-phosphate. It has been shown to have high purity and high quality, and is suitable for use in diagnostics, food testing, and as a bioluminescence substrate. Aldol 535 is also used as a ligand in various enzyme assays. It has been tested for its ability to react with the fluorogenic substrates: 4-methylumbelliferyl phosphate and 4-methylumbelliferone phosphate. The CAS number is 2484872-80-0.</p>

<p>Aldol 495 is a chemiluminescent substrate for the detection of aldehyde reductase (ALR) activity. It is an acetate ester that reacts with ALR to form an aldehyde product, which then reacts with luminol in the presence of hydrogen peroxide to produce light. Aldol 495 is used in the diagnosis of ALR deficiency, which can cause acute intermittent porphyria and other diseases. Aldol 495 has been shown to be non-toxic and non-mutagenic.</p>