Substratos enzimáticos

<p>Fluorescent dye substrate for flow cytometric measurements</p>

<p>4-(Bromomethyl)phenyl-beta-D-triacetylglucuronic acid methyl ester, also known as 4-bromo-4'-(N,N-dimethylamino)biphenyl, is a bioluminescent substrate. It is used for the detection of beta-glucuronidase activity in biological samples and for the detection of bacterial β-glucuronidase activity in culture media. Beta-glucuronidase catalyzes the hydrolysis of glucuronic acid by breaking down a conjugate molecule into two smaller molecules, which can be detected using bioluminescence. 4-(Bromomethyl)phenyl-beta-D-triacetylglucuronic acid methyl ester is a chromogenic substrate that has been shown to be an excellent ligand in biomolecular research because it reacts with many different enzymes. This product is highly pure and</p>

<p>5-Bromo-6-chloro-3-indolyl a-D-galactopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in glycolipid metabolism studies.</p>

<p>2-Nitrophenyl b-D-fucopyranoside is a chromogenic substrate used in various enzymatic assays to detect the presence of fucosidase enzymes. It consists of a nitrophenyl group attached to a fucose sugar molecule. When acted upon by enzymes such as fucosidases, it releases the colored nitrophenyl group, which can be detected using spectrophotometry.</p>

<p>Resorufin b-D-xylobioside is a fluorogenic substrate for xylanases useful for xylanase activity assays.</p>

<p>DMNPE-caged form of luciferin</p>

<p>Fluorogenic substrate targeting beta-glucosidase</p>

<p>A fluorogenic substrate for detection of enzymatic activity of the SARS-CoV-2 Mpro protease. The fluorophore 7-Amino-4-methylcoumarin (AMC) is linked to the tetrapeptide via a peptide bond at the C-terminal of the glutamine residue. Following the hydrolytic activity of the Mpro protease, the fluorogenic AMC group get released from the substrate, generating a fluorescent signal (Ex 380 nm / Em 455 nm).</p>

<p>4-Methylumbelliferyl 2-deoxy-2-sulfamino-a-D-glucopyranoside sodium salt (M3A or MU-GlcNS) is a fluorogenic substrate of heparin sulfamidase, used in the diagnosis of Mucopolysaccharidosis III type A (MPS III A). MPS III A, also known as Sanfilippo syndrome type A, is caused by the deficiency of heparan N-sulfatase. Heparin sulfamidase in the assay desulfates MU-GlcNS to 4-methylumbelliferyl 2-amino-2-deoxy-α-D-glucopyranoside (MU-GlcNH2). To release fluorometrically measurable 4-methylumbelliferone (MU) and by-product glucosamine, MU-GlcNH2 is further hydrolysed by an enzyme such as yeast α-glucosidase during a second incubation.</p>

<p>4-Methylumbelliferyl alpha-D-glucosaminide is a fluorogenic substrate for alpha-N-acetylglucosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl alpha-D-glucosaminideas a substrate for measuring the alpha-N-acetylglucosaminidase activity is used for Sanfilippo syndrome B and Mucopolysaccharidosis IIIB (MPS IIIB) diagnosis.</p>

<p>Chromogenic substrate for β-D-Cellobiosidase yielding a yellow solution upon cleavage.</p>

<p>Chromogenic substrate for fucosyltransferases</p>

<p>5-Bromo-4-chloro-3-indoxyl oleate is a chromogenic substrate used to detect the specific enzymatic activity of lipase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl oleate is used in lipid metabolism disorder screening.</p>

<p>6-Chloro-3-indolyl 2-acetamido-2-deoxy-b-D-galactopyranoside is a chromogenic substrate for N-acetyl-beta-galactosaminidase. It produces a salmon-pink color after enzymatic cleavage.</p>

<p>6'-(Diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl b-D-glucopyranoside is a fluorogenic substrate that is used in diagnostic applications. It has been shown to be a useful ligand for the determination of enzyme activity, and it can also be used to detect bacterial culture media contaminants. 6'-(Diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl b-D-glucopyranoside is used as an enzyme substrate in chemiluminescent assays, and with other reagents, it can be used for the detection of environmental contaminants. This product has a CAS number of 55426-02-8 and can be found on the website www.sigmaaldrich.com/catalog/product</p>

<p>4-Nitrophenyl decanoate is a chromogenic substrate for lipase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl decanoate is used in different applications such as the Pancreatic insufficiency diagnosis, lipid metabolism studies</p>

<p>Aldol® 515 palmitate is a chromogenic substrate that is used in food testing, diagnostics, and other applications. It has a high purity and is conjugated to various ligands for specific applications. Aldol® 515 palmitate is an excellent chemiluminescent substrate for the detection of ATP and other nucleotides. It also has high bioluminescence activity for the detection of adenosine-5'-triphosphate (ATP) or luciferin as well as enzyme substrates to measure β-galactosidase or β-glucuronidase activity.</p>

<p>5-Bromo-6-chloro-3-indolyl-2-acetamido-2-deoxy-a-D-glucopyranoside is a chemiluminescent substrate that is used as an indicator in diagnostic tests. This compound reacts with an oxidizing agent, such as hydrogen peroxide, to generate light. It is also used as a chromogenic substrate for the detection of bioluminescence and it can be applied in culture media or food testing. 5Bromo-6Chloro-3Indolyl2Acetamido2DeoxyA1DGlucopyranoside has a CAS number, which means that it meets the standards set by the Chemical Abstracts Service. It also has a high purity and quality, making it suitable for use in laboratories.</p>

<p>Fluorogenic substrate for esterase yielding a blue fluorescent solution upon cleavage.</p>

<p>Also known as Lapis-caprylate; targeting esterase with C8 activity; Blue color</p>