Substratos enzimáticos
Substratos enzimáticos são moléculas sobre as quais as enzimas atuam para catalisar reações químicas. O substrato liga-se ao sítio ativo da enzima, onde passa por uma transformação específica para formar o produto da reação. Os substratos enzimáticos são ferramentas essenciais na pesquisa para estudar a cinética enzimática, especificidade e mecanismo de ação. Eles também são usados em ensaios diagnósticos e estudos bioquímicos. Na CymitQuimica, oferecemos uma seleção diversificada de substratos enzimáticos de alta qualidade para apoiar suas pesquisas em enzimologia e bioquímica.
Subcategorias de "Substratos enzimáticos"
Foram encontrados 1528 produtos de "Substratos enzimáticos"
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6-Chloro-3-indoxyl-alpha-D-glucopyranoside
CAS:Chromogenic substrate for α-glucosidase yielding a salmon-colored precipitate.Fórmula:C14H16ClNO6Pureza:Min. 98 Area-%Peso molecular:329.74 g/molAldol® 458 nonanoate solution, 0.75 M in DMSO, Biosynth Patent: EP 2427431 and US 8940909
CAS:Produto ControladoAldol 458 nonanoate solution is a high quality, enzyme substrate that can be used for a number of applications. It has been shown to be an excellent chromogenic substrate, bioluminescence, ligand and chemiluminescence. Aldol 458 nonanoate solution has also been used for the detection of staining and diagnostics. The compound is a conjugate with an excellent binding affinity to proteins. This product can be used in food testing, culture media, fluorogenic substrate and environmental testing. CAS No. 2484873-15-4Fórmula:C25H29NO4Pureza:Of Solute (Hplc) 98.0 To 100.0 Area-%Peso molecular:407.51 g/molAldol® 470 phosphate, disodium salt, Biosynth Patent: EP 2427431 and US 8940909
CAS:Aldol 470 phosphate is a chromogenic substrate for phosphatases yielding a yellow precipitate. Furthermore it shows some fluorescence (excitationat 320 nm to 490 nm). In contrast to classical indoxyl substrates, Aldolsubstrates work not only under aerobic but also under anaerobic conditions.Fórmula:C23H18NNa2O7PPureza:Min. 95.0 Area-%Peso molecular:497.34 g/molbeta-Mannachrome
Beta-mannachrome is a chemical compound with the formula of CHN(CH)CO. It is a colorless solid that has been used for staining, as a ligand in coordination chemistry and as a biological substrate for chemiluminescence. Beta-mannachrome is used in diagnostics and as an enzyme substrate in various biochemical reactions.
Beta-Mannachrome is an organic compound that can be synthesized from benzaldehyde and mannitol. It is also found in nature, where it is produced by the fungus Cladosporium cladosporioides. Beta-Mannachrome reacts with hydrogen peroxide to produce light through chemiluminescence. The light produced by this reaction can be measured using a luminometer or other optical instruments to determine the concentration of beta-Mannachrome present.
The CAS number of beta-Mannachrome is 619-96-8 and its molecular weight is 134.17Pureza:Min. 95%Tyramine
CAS:Tyramine targets peroxidase to form a brown colorFórmula:C8H11NOCor e Forma:Off-White Slightly Brown PowderPeso molecular:137.18 g/mol2-(2-Thiazolylazo)-p-cresol
CAS:2-(2-Thiazolylazo)-p-cresol structure enables it to form complexes with certain metal ions, changing color in the process. This color change is highly useful in titrimetric analyses (quantitative chemical analysis method) to determine the concentration of metal ions in a solution. The substrates are colored orange-yellow (absorbance maximum= 375 nm). After the enzymatic conversion, the resulting TAC shows an intense, soluble red-violet color (544 nm) in an alkaline medium (pH = 10.3).Fórmula:C10H9N3OSPureza:Min. 96 Area-%Cor e Forma:Yellow To Orange Brown SolidPeso molecular:219.26 g/mol2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt
CAS:ABTS can be used as a peroxidase substrate that gives a metastable cation when in the presence of H2O2. 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) or ABTS is commonly used in the enzyme-linked immunosorbent assay (ELISA). ABTS is chosen because the enzyme facilitates the reaction to give a green end-product with an absorbance maximum of 420nm and can therefore be detected with a spectrophotometer.Fórmula:C18H24N6O6S4Pureza:Min. 98.0 Area-%Cor e Forma:PowderPeso molecular:548.68 g/molResorufin N-acetyl-b-D-galactosamine
Resorufin N-acetyl-b-D-galactosamine is a fluorogenic substrate used as a probe to detect and quantify the activity of N-acetyl-beta-D-galactosaminidase (also known as beta-hexosaminidase or HEX(NAG)). Particularly useful in biomedical research, clinical chemistry, and diagnostics, when the substrate is cleaved, the highly fluorescent resorufin dye is produced that can be detected at approximately 598 nm.Pureza:Min. 95%Cor e Forma:PowderPeso molecular:416.38 g/molFast blue B salt
CAS:For visualizing enzymatic activityFórmula:C14H12N4O2Cl2·ZnCl2Cor e Forma:PowderPeso molecular:475.46 g/mol3-(2'-Spiroadamantane)-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane
CAS:As an alternative to commonly used colorimetric and fluorescent substrates, 3-(2'-Spiroadamantane)-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane, abbreviated AMPPD, is a chemiluminescent substrate for alkaline phosphatase (ALP). It allows detection of alkaline phosphatase at a high sensitivity. Literature reports that the co-presence of 0.1% bovine serum albumin (BSA) leads to a signal increase of 10-folds under selected conditions.Fórmula:C18H23O7PPureza:Min. 95%Cor e Forma:PowderPeso molecular:382.34 g/mol[2-(2-Thiazolylazo)-4-cresyl]phosphate, p-toluidine sal
2-(2-Thiazolylazo)-4-cresyl]phosphate, p-toluidine salt is a chromogenic substrate for alkaline phosphatase resulting in a dark red color.Pureza:Min. 95%4-Methylumbelliferyl a-D-arabinopyranoside
A fluorogenic MOSCERDAM® substrate targeting alpha-arabinofuranosidasePureza:Min. 95%Peso molecular:308.28 g/mol5-Bromo-3-indolyl b-D-glucopyranoside
CAS:5-bromo-3-indolyl-b-d-glucopyranoside, also known as 5BIG, is an enzyme substrate for beta-glucosidase. It is used in bacterial assays for the identifcation of Gram-positive bacteria such as Staphylococcus aureus and Bacillus subtilis. After cleavege, it gives an intense blue color.Fórmula:C14H16BrNO6Pureza:Min. 98 Area-%Cor e Forma:PowderPeso molecular:374.18 g/molAquaSpark® β-D-galactoside, 10 mM in DMSO, Ramot at Tel-Aviv University Ltd. Patent family WO 2017/130191
CAS:AquaSpark® beta-D-galactoside, 10 mM in DMSO is a fluorogenic substrate for beta-galactosidase. This is typically used at 10 – 50 µM final concentration. Cymit Quimica provides AquaSpark® beta-D-galactoside as a 10 mM stock solution in dimethyl sulfoxide (DMSO) for convenience and improved storage stability.Fórmula:C34H39ClO12Pureza:Min. 95%Peso molecular:675.12 g/molN-Benzoyl-DL-arginine 4-nitroanilide hydrochloride
CAS:N-α-benzoyl-DL-arginine-4-nitroanilide hydrochloride is commonly used as a substrate in various enzymatic assays, particularly for studying proteases like trypsin and chymotrypsin. It serves as a synthetic substrate to measure the activity of these enzymes. For instance, it has been used to determine trypsin activity in different organisms and tissues. The substrate has also been employed in studying the enzymatic properties of various proteases, including those found in latex, snake venoms, and plant seeds. Additionally, it has been utilized to assess the effects of different compounds on trypsin activity, such as condensed tannins and wine.
Fórmula:C19H22N6O4•HClPureza:Min. 95%Cor e Forma:PowderPeso molecular:434.88 g/mol8-Hydroxyquinoline b-D-galactopyranoside
CAS:Chromogenic substrate targeting the beta-galactosidase enzymeFórmula:C15H17NO6Pureza:Min. 98 Area-%Cor e Forma:PowderPeso molecular:307.3 g/molAldol® 470 L-pyroglutamic acid amide, Biosynth Patent: EP 2427431 and US 8940909
CAS:Produto ControladoAldol® 470 L-pyroglutamic acid amide is a chromogenic and fluorogenic enzyme substrate used for detection of pyrrolidonyl peptidase (PYRase) activity. The colorless enzyme substrate is cleaved by PYRase-producing bacteria grown in liquid or solid media, yielding orange color. In addition, green fluorescence is generated when a suitable matrix is present, such as, the surface of cellulose acetate filters, polypropylene tubes or microplates, and polyethylene vials. Aldol® 470 L-pyroglutamic acid amide is suitable for use under aerobic and anaerobic conditions.Fórmula:C28H25N3O5Pureza:Min. 95%Cor e Forma:PowderPeso molecular:483.52 g/mol4-Methylumbelliferyl phosphate dilithium
CAS:4-Methylumbelliferyl phosphate dilithium is a fluorogenic substrate for alkaline phosphatase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl phosphate dilithiumas a substrate for measuring the alkaline phosphatase activity is used for bone metabolism disorder studies and hypophosphatasia diagnosis.Fórmula:C10H7O6P•Li2Pureza:Min. 95%Cor e Forma:PowderPeso molecular:268.01 g/mol5-Bromo-6-chloro-3-indoxyl choline phosphate
CAS:5-Bromo-6-chloro-3-indoxyl choline phosphate is a chemiluminescent substrate for the detection of bacterial activity. It reacts with an enzyme, luciferase, which is present in a variety of living cells. The reaction produces light. This product has been tested for environmental and food safety by the manufacturer. 5-Bromo-6-chloro-3-indoxyl choline phosphate is used as a diagnostic tool to determine the presence of bacteria in humans, animals, and plants. It can be used to measure levels of bacteria in culture media, water samples, and food products. 5-Bromo-6-chloro-3-indoxyl choline phosphate can also be used as a substrate for enzymes such as phosphatases or esterases that catalyze reactions with substrates with brominated or chlorinated groups.Fórmula:C13H17BrClN2O4PPureza:Min. 95%Peso molecular:411.63 g/molResorufin acetate
CAS:Resorufin acetate is a fluorogenic substrate for esterase activity measurments in lipid metabolism studies. It is also used as a substrate for chymotrypsin, a digestive enzyme produced by the pancreas that aids in protein digestion in the small intestine.
Fórmula:C14H9NO4Pureza:Min. 95%Cor e Forma:PowderPeso molecular:255.23 g/mol
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