Substratos enzimáticos

4-Nitrophenyl 2-acetamido-3-O-(2,3,4,6-tetra-O-acetyl-b-D-galactopyranosyl)-2-deoxy-a-D-galactopyranoside is a chromogenic substrate designed for the detection and quantification of enzyme activity. This tetra-acetylated compound is specifically designed to be cleaved by enzymes, releasing a yellow 4-nitrophenol product that can be easily measured spectrophotometrically, allowing for accurate and sensitive analysis of enzyme kinetics and inhibition studies.

4-Nitrophenyl 4,6-O-benzylidene-3-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-b-D-glucopyranoside is a high purity fluorescent reagent that can be used as a substrate for many enzymes. It is also used in environmental testing and to detect the presence of nitrophenols in food. This product has CAS number 545414.

4-Nitrophenyl 6-O-DMT-2,3,4-tri-O-levulinoyl-a-D-mannopyranoside is a fluorogenic substrate that can be used for the detection of DMT (dihydroxyacetone) in food products. It has been shown to be an excellent alternative to the more commonly used 4,5′,6,7,8,-Tetrabromoindoxyl 3′,5′,6′,-tetraacetic acid (TBA). This product is also used as a chromogenic substrate for the determination of phenols in environmental testing and as a ligand conjugate for immunohistochemistry staining. The purity of this product is guaranteed and it has been tested extensively.

Bacillus cereus is a rod-shaped β hemolytic bacterium commonly found in food and soil. Some strains can be associated with food intoxications, mainly from raw meat and poultry products. One example is the so called “fried rice syndrome”, that occurs when dishes containing cooked rice are left for several hours at room temperature. Typical symptoms of the infection include severe nausea, vomiting and diarrhea. Some strains of Bacillus cereus on the contrary are not harmful at all and they are used as probiotics food additives in animal farming to reduce the presence of other pathogens like Salmonella in the cecum end intestine. Bacillus cereus expresses phosphatidyl-choline specific phospholipase C (PC-PLC), encoded by the plc gene, which is regulated by PlcR. In addition, the expression of PlcR is dependent on the growth medium. Luc-B.cereus Na (L-8275) is a synthetic chemiluminescent substrate for the phosphatidyl-choline specific phospholipase C. The enzyme cleaves off phosphocholine from L-8275, leading to the release of D-Luc, and consequent strong light emission B. cereus culture exhibited strong luminescence, while RLU values were much lower for samples with B. thuringiensis.

4-Methylumbelliferyl 2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)-b-D-galactopyranoside is a fluorescent substrate for β–galactosidase. It is used to detect the enzyme in biological samples and as a diagnostic agent. This substrate can be used to develop chemiluminescent, chromogenic, and fluorogenic substrates for use in diagnostics. 4MUF 2OBG can also be conjugated to various ligands for use in bioluminescence assays or as a marker in immunoassays.

Aldol® 518 myo-inositol-1-phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-inositol specific phospholipase C activity (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC or PI-PLC-positive microorganisms grown in liquid or solid media, yielding an intense red coloration. In combination with Aldol® 355 fluorescence enhancer, red fluorescence can also obtained. Aldol® 518 myo-inositol-1-phosphate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.