Substratos enzimáticos
Substratos enzimáticos são moléculas sobre as quais as enzimas atuam para catalisar reações químicas. O substrato liga-se ao sítio ativo da enzima, onde passa por uma transformação específica para formar o produto da reação. Os substratos enzimáticos são ferramentas essenciais na pesquisa para estudar a cinética enzimática, especificidade e mecanismo de ação. Eles também são usados em ensaios diagnósticos e estudos bioquímicos. Na CymitQuimica, oferecemos uma seleção diversificada de substratos enzimáticos de alta qualidade para apoiar suas pesquisas em enzimologia e bioquímica.
Subcategorias de "Substratos enzimáticos"
Foram encontrados 1527 produtos de "Substratos enzimáticos"
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2-Chloro-4-nitrophenyl α-D-mannopyranoside
CAS:2-Chloro-4-nitrophenyl alpha-D-mannopyranoside is a substrate used to measure the alpha-mannosidase activity. This enzyme plays a crucial role in the lysosomal degradation of asparagine-linked carbohydrate cores of glycoproteinsis. The use of 2-Chloro-4-nitrophenyl alpha-D-mannopyranoside in diagnostic assays is important as a deficiency in alpha-mannosidase leads to the accumulation of mannose-rich oligosaccharides in lysosomes, causing a range of health problems collectively known as alpha-mannosidosis.
Fórmula:C12H14ClNO8Pureza:Min. 98 Area-%Cor e Forma:White PowderPeso molecular:335.69 g/mol4-Nitrophenyl b-L-arabinofuranoside
CAS:Chromogenic substrate targeting alpha-L-arabinofuranosidase
Pureza:Min. 95%Cor e Forma:White/Off-White SolidPeso molecular:271.22 g/mol4-Methylumbelliferyl 2-acetamido-2-deoxy-α-D-glucopyranoside-6-sulfate potassium
CAS:4-Methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside-6-sulfate potassium is a fluorogenic substrate for N-acetylglucosamine-6-sulfatase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside-6-sulfate potassiumas a substrate for measuring the N-acetylglucosamine-6-sulfatase activity is used for screening of Mucopolysaccharidosis IIID (MPS IIID) (a rare lysosomal storage disorder) and Sanfilippo D syndrome diagnosis.Fórmula:C18H20KNO11SPureza:Min. 95%Cor e Forma:White PowderPeso molecular:497.52 g/molResorufin-β-D-glucuronic acid, sodium salt
CAS:Resorufin-beta-D-glucuronic acid, sodium salt is a fluorogenic substrate that can be used in diagnostic applications. It is a conjugate of resorufin and beta-D-glucuronic acid. The enzyme substrate reacts with the enzyme luciferase to produce light. The high purity and quality of this product make it ideal for use in the detection of the presence of glucose in urine, which can be used to diagnose diabetes mellitus.Fórmula:C18H14NNaO9Pureza:Min. 90.0 Area-%Peso molecular:411.30 g/molRef: 3D-R-2150
2mgA consultar5mgA consultar10mgA consultar25mgA consultar50mgA consultar-Unit-ggA consultar4-Nitrophenyl-α-D-maltopyranoside
CAS:Chromogenic α-maltosidase substrate yielding a yellow solution upon cleavage.Fórmula:C18H25NO13Pureza:Min. 98.0 Area-%Peso molecular:463.40 g/molGlutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride
CAS:Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for aminopeptidase.
Fórmula:C23H30N6O7·HClPureza:Min. 95%Cor e Forma:PowderPeso molecular:538.98 g/mol4-Nitrophenyl 2-acetamido-2-deoxy-b-D-glucopyranoside-6-sulfate
4-Nitrophenyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chemiluminescent substrate that can be used in the detection of various enzymes. This product has been tested for sterility and purity, and it has passed all tests. It is also free of any detectable levels of heavy metals or other contaminants. 4NP2AG6S is a high quality bioluminescent substrate with CAS No. 71498-92-5. It is a fluorescent compound that reacts with an enzyme to produce light. The reaction can be detected by measuring the emitted light intensity at 680 nm (max).Pureza:Min. 95%4-Nitrophenyl-beta-D-mannopyranoside
CAS:Chromogenic β-mannosidase substrate yielding a yellow solution upon cleavage.Fórmula:C12H15NO8Pureza:Min. 98.0 Area-%Peso molecular:301.26 g/molN-α-CBZ-L-Arginine 7-amido-4-methylcoumarin hydrochloride
CAS:N-alpha-CBZ-L-Arginine 7-amido-4-methylcoumarin hydrochloride is a high quality and highly purified substrate for enzymes that catalyze the release of light when they react with their specific substrates. It is used in a variety of applications, including diagnostics and food testing. NACBZLAMC hydrochloride is an excellent ligand for chemiluminescence reactions and can be used to detect the presence of various analytes. It can also be used as a staining agent to detect proteins on Western blots or to stain bacterial cultures for identification. This product has been shown to be effective against numerous bacteria, including Staphylococcus aureus and Pseudomonas aeruginosa, as well as fungi like Candida albicans.Fórmula:C24H28ClN5O5Pureza:Min. 99 Area-%Peso molecular:501.97 g/molRef: 3D-C-2100
1gA consultar5gA consultar10gA consultar500mgA consultar2500mgA consultar-Unit-ggA consultar2-Chloro-4-nitrophenyl phosphate
CAS:2-Chloro-4-nitrophenyl phosphate is a chromogenic substrate for phosphatases. Afer cleavage of the substrate, 2-chloro-4-nitrophenol is released, which can be quantified at 405 nm. 2-Chloro-4-nitrophenyl phosphate is used in clinical diagnostics to for prostate cancer, diagnosis of liver and bone diseases.Fórmula:C6H5ClNO6PPureza:Min. 95 Area-%Peso molecular:253.53 g/molN-(3-Sulfopropyl)-3,3',5,5'-tetramethylbenzidine sodium
CAS:N-(3-Sulfopropyl)-3,3',5,5'-tetramethylbenzidine (TMB) sodium salt is a highly sensitive chromogenic substrate used for the detection and quantification of peroxidase enzyme activity. This water-soluble substrate undergoes a color change from colorless to blue upon oxidation by peroxidase enzymes in the presence of hydrogen peroxide. The reaction can be stopped by adding an acid, resulting in a yellow color that can be measured spectrophotometrically. TMB sodium salt is widely used in various applications, including enzyme-linked immunosorbent assays (ELISA), immunohistochemistry, and other diagnostic tests, due to its high sensitivity, low background signal, and excellent stability.
Pureza:Min. 95%Cor e Forma:PowderPeso molecular:384.47 g/molD-Luciferin Firefly
CAS:Substrate for firefly luciferase used for imaging the expression of the LUC marker gene and luciferase-fusion conjugates in living cells, tissues and organisms. Extensively utilized in reporter gene assays, immunoassays and hygiene monitoring.
Fórmula:C11H8N2O3S2Pureza:Min. 99.0 Area-%Peso molecular:280.33 g/molRef: 3D-L-8200
1gA consultar10mgA consultar250mgA consultar500mgA consultar2500mgA consultar-Unit-ggA consultar3-Nitrophenyl b-D-galactopyranoside
CAS:Chromogenic substrate targeting beta-galactosidaseFórmula:C12H15NO8Cor e Forma:PowderPeso molecular:301.25 g/mol3-Indoxyl caprylate
CAS:3-Indoxyl caprylate is a chemiluminescent substrate that is used in the detection of bacteria. It has been shown to be a very sensitive and selective fluorogenic substrate for the detection of bacterial activity, which can be used with an appropriate enzyme reaction. 3-Indoxyl caprylate has also been shown to be a potent quencher of bioluminescence, and can be used as a chromogenic substrate in diagnostic assays. 3-Indoxyl caprylate is often used as a culture media supplement or food additive to enhance its growth properties. It has been found to have high purity and quality levels, making it suitable for use in diagnostics and research.Pureza:Min. 95%Peso molecular:259.34 g/mol4-Nitrophenyl α-D-glucuronide
CAS:4-Nitrophenyl alpha-D-glucuronide is a chromogenic substrate widely used for detecting and quantifying the activity of alpha glucuronidase enzyme. Upon cleavage by the enzyme, it releases the yellow-colored 4-nitrophenol, whose absorbance can be monitored spectrophotometrically. This substrate is routinely employed in biochemistry and molecular biology applications, such as enzyme assays, enzyme kinetics studies, and characterization of recombinant enzymes. As a non-toxic and easy-to-use reagent, 4-Nitrophenyl alpha-D-glucuronide provides a convenient and sensitive method for studying alpha-glucuronidase activity in various biological samples, including cell extracts, tissue homogenates, and purified enzyme preparations.
Fórmula:C12H13NO9Pureza:Min. 97 Area-%Cor e Forma:Off-White PowderPeso molecular:315.23 g/mol1-Methyl 2-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)ethanedioate
CAS:Inorganic phosphate targeted colorimetric probe
Fórmula:C13H10O6Pureza:Min. 95%Peso molecular:822.35 g/mol4-Methylumbelliferyl myo-inositol-1-phosphate, N-methyl-morpholine salt
CAS:Fluorogenic substrate for phosphatidylinositol-specific phospholipase C. Isolation and identification of Listeria monocytogenes, bacteria of the Bacillus cereus group, yeasts and molds. See also B-7404.Fórmula:C21H30NO12PPureza:Min. 98.0 Area-%Peso molecular:519.45 g/mol4-Nitrophenyl 2-acetamido-6-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-3-O-(b-D-galactopyranosyl)-2-deoxy-a-D-galactopyranoside
CAS:4-Nitrophenyl 2-acetamido-6-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-3-O-(b-D-galactopyranosyl)-2-deoxy-a-D-galactopyranoside is a specific chromogenic substrate designed for the highly sensitive and accurate detection of glycosidase enzyme activity. Glycosidases are responsible for the cleavage of glycosidic bonds between saccharide units in various carbohydrates. The hydrolysis of this substrate by glycosidase enzymes produces 4-nitrophenol, a yellow-colored compound that can be easily monitored through spectrophotometric measurements due to its strong absorbance at 405 nm. This chromogenic substrate is widely used in the analysis of glycosidase activity in various biological samples, including bacterial, fungal, and plant tissues, as well as in environmental samples and biotechnological applications.Fórmula:C28H41N3O18Pureza:Min. 95 Area-%Cor e Forma:White PowderPeso molecular:707.63 g/mol4-Methylumbelliferyl caprylate
CAS:Fluorogenic substrate for esterase with C8 activity yielding a blue fluorescent solution upon cleavage. Used for the identification of Salmonella spp. based on the detection of caprylate esterase.Fórmula:C18H22O4Pureza:Min. 98 Area-%Peso molecular:302.37 g/molRef: 3D-M-5526
1gA consultar5gA consultar10gA consultar25gA consultar2500mgA consultar-Unit-ggA consultar4-Methylumbelliferyl acetate
CAS:Fluorogenic substrate for carboxylesteraseFórmula:C12H10O4Pureza:Min. 95%Cor e Forma:White PowderPeso molecular:218.21 g/molL-Histidine 7-amido-4-methylcoumarin hydrochloride
CAS:L-Histidine 7-amido-4-methylcoumarin hydrochloride is a fluorogenic substrate, which is a synthetic chemical that is used as a substitute for natural substrates. It reacts with enzymes in the presence of hydrogen peroxide to produce light. This product has been used as an enzyme substrate, a diagnostic tool and has been used to detect bacterial growth in culture media. L-Histidine 7-amido-4-methylcoumarin hydrochloride is also used for food testing and environmental testing, where it can be used as a chromogenic substrate or conjugate respectively. This product has CAS number 5981-00-6 and molecular weight of 254.27 g/mol.Fórmula:C16H17ClN4O3Peso molecular:348.79 g/mol5-Bromo-6-chloro-3-indoxyl butyrate
CAS:5-Bromo-6-chloro-3-indoxyl butyrate is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in lipid digestion assays.Fórmula:C12H11BrClNO2Pureza:Min. 95%Cor e Forma:PowderPeso molecular:316.58 g/mol4-Methylumbelliferyl a-L-idopyranosiduronic acid 2-sulphate disodium salt - Moscerdam™ biochemical purity
CAS:PAIRED PRODUCT AVAILABLE: alpha-L-iduronidase Enzyme, FI1793834MU-alpha-L-iduronide-2-sulphate (M2) is a fluorogenic substrate of iduronate-2-sulphatase (IDS), used in a 2-step assay coupled with alpha-L-iduronidase. The assay is used in biochemical testing for Hunter syndrome (mucopolysaccharidosis II / MPS II). In a first step, IDS from biological samples cleaves the sulphate group from M2. In a second step, exogeneous alpha-L-iduronidase which cleaves the 4-MU moiety is added to the reaction, resulting in release of a fluorescent signal (Ex 360 nm / Em 455 nm). The reaction is stopped by the addition of sodium carbonate.Fórmula:C16H14Na2O12SPureza:Min. 99 Area-%Cor e Forma:White PowderPeso molecular:476.32 g/mol6-Chloro-3-indoxyl-beta-D-galactopyranoside, plant origin
CAS:6-Chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase that yields a salmon or rose-coloured precipitate and hence is also known as Salmon-Gal. It can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside (IPTG) as an alternative to the chromogenic substrate 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-Gal) for detection of beta-galactosidase activity in bacterial colonies in colorimetric assays. Where beta-galactosidase expressed from a reporter gene in molecular biology experiments, recombinant colonies on growth plates are salmon-coloured while non-recombinants appear as white. Use of nitroblue tetrazolium salt (NBT) with Salmon-Gal may provide faster results, yielding a clear, purple precipitate, than for Salmon-Gal alone. In conjunction with 5-bromo-4-chloro-3-indolyl-beta-D-glucoside (X-Glc), Salmon-Gal is useful for simultaneous detection of GUS and Lac activities in the same medium.Fórmula:C14H16ClNO6Pureza:Min. 98.0 Area-%Peso molecular:329.74 g/mol4-Nitrophenyl b-D-ribofuranoside
CAS:4-Nitrophenyl β-D-ribofuranoside is a synthetic chromogenic molecule designed to profile phosphorolytic enzyme activity, specifically ribonucleoside-hydrolyzing enzymes. Upon hydrolysis by these enzymes, it releases a 4-nitrophenolic compound, which can be monitored spectrophotometrically at 405-410 nm as a quantitative indicator of enzyme activity. This substrate is suitable for studying ribonucleoside-hydrolyzing enzymes, such as ribo- and deoxyribonucleases, in various biological systems and, in turn, could help advance our understanding of nucleic acid metabolism, gene function, and regulatory processes.Fórmula:C11H13NO7Pureza:Min. 97 Area-%Cor e Forma:White PowderPeso molecular:271.22 g/mol3-Indoxyl-beta-D-glucopyranoside anhydrous
CAS:The maunfacturing of blue jeans started 150 years ago as workwear, and are found nowadays in almost every wardrobe. Their renowned indigo color tone is truly unique; nevertheless, the high demand for the dye poses serious sustainability and environmental problems connected with its manufacturing process. Recently, a group of scientists presented an alternative indigo dyeing method inspired by the indoxyl glucoconjugate present in the plant P.tinctorium. The researchers proposed as an alternative approach a biocatalytic process using recombinant E.coli to access water soluble β-gluco-indoxyl (indican). At the point of coloring, indican gets hydrolyzed by β-glucosidase enzymes forming indoxyl, which suddenly undergoes oxidation to leucoindigo and further crystallizes to indigo giving the typical blue denim its signature character.Fórmula:C14H17NO6Pureza:Min. 99.0 Area-%Peso molecular:295.29 g/mol7-((4'-L-Alaninamido)-rac-3'-hydroxybutyloxy) coumarin hydrochloride
This Clips-O substrate 7-((4'-L-Alaninamido)-rac-3'-hydroxybutyloxy) coumarin allows the detection of single and dual enzymatic activity (L-alanine aminopetidase and oxidation of 4-amino-3-hydroxybutyloxy spacer either enzymatically or by exposure to sodium periodate) with a single substrate resulting in a fluorescent signal. The main virtues of Clips-O substrates are an increased stability towards non-enzymatic hydrolysis that results in a reduced background and high sensitivity.Fórmula:C16H21ClN2O5Peso molecular:356.8 g/mol3-Indoxyl caprylate
CAS:3-Indoxyl caprylate is an enzyme substrate used in a variety of diagnostic applications, including the detection of various bacterial and viral infections. It is used as a substrate for beta-galactosidase, which can be detected by bioluminescence or chromogenic substrates. 3-Indoxyl caprylate also serves as a ligand for receptor binding and has been shown to bind to CD4 receptors on human T cells. 3-Indoxyl caprylate is also used as an enzyme substrate in the detection of DNA polymerases, RNA polymerases, and ATPases. This product is available with a high purity that ranges from 98% to 99%. 3-Indoxyl caprylate is soluble in water, alcohols, acetone, acetic acid, and chloroform.Fórmula:C16H21NO2Peso molecular:259.35 g/molRef: 3D-I-5690
1gA consultar5gA consultar10gA consultar500mgA consultar2500mgA consultar-Unit-ggA consultar4-(Bromomethyl)phenyl-β-D-triacetylglucuronic acid methyl ester
4-(Bromomethyl)phenyl-beta-D-triacetylglucuronic acid methyl ester, also known as 4-bromo-4'-(N,N-dimethylamino)biphenyl, is a bioluminescent substrate. It is used for the detection of beta-glucuronidase activity in biological samples and for the detection of bacterial β-glucuronidase activity in culture media. Beta-glucuronidase catalyzes the hydrolysis of glucuronic acid by breaking down a conjugate molecule into two smaller molecules, which can be detected using bioluminescence. 4-(Bromomethyl)phenyl-beta-D-triacetylglucuronic acid methyl ester is a chromogenic substrate that has been shown to be an excellent ligand in biomolecular research because it reacts with many different enzymes. This product is highly pure and
Fórmula:C20H23BrO10Pureza:Min. 95 Area-%Peso molecular:503.29 g/mol4-Methylumbelliferyl dodecanoate
CAS:4-Methylumbelliferyl dodecanoate is a fluorogenic substrate for lipase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl dodecanoateas a substrate for measuring the lipase activity is used for detecting pancreatic insufficiency in diagnosis assays as well as the screening of lipase deficiency.Fórmula:C22H30O4Pureza:Min. 95%Cor e Forma:PowderPeso molecular:358.47 g/mol5-Bromo-4-chloro-3-indolyl b-D-galactofuranoside
5-Bromo-4-chloro-3-indolyl b-D-galactofuranoside is a chromogenic substrate used to detect the specific enzymatic activity of beta-D-galactofuranosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl b-D-galactofuranoside is used in assays for detecting bacterial pathogens (e.g., Mycobacterium tuberculosis).Fórmula:C14H15BrClNO6Pureza:Min. 95%Cor e Forma:PowderPeso molecular:408.63 g/molOstazin brilliant red H-3B starch
Ostazin Brilliant Red H-3B is a water soluble, non-toxic, and non-hazardous substrate for enzymes that catalyze reactions involving the cleavage of β-D-glucosides. It is used as a chromogenic or fluorogenic substrate in diagnostic tests. Ostazin Brilliant Red H-3B can also be used in bioluminescence reaction with luciferase to measure ATP levels in living cells.Pureza:Min. 95%L-Lysyl-L-alanine 7-amido-4-methylcoumarin dihydrochloride
CAS:L-Lysyl-L-alanine 7-amido-4-methylcoumarin dihydrochloride is a fluorogenic peptide substrate for aminopeptidase. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.Pureza:Min. 98.0 Area-%Cor e Forma:Powder4-Nitrophenyl b-D-cellotetraoside
CAS:4-Nitrophenyl b-D-cellotetraoside is a chromogenic substrate utilized for the detection and quantification of cellulase enzyme activity. Cellulase enzymes hydrolyze the beta-1,4-glycosidic bonds of cellulose, a major component of plant cell walls. Upon cleavage by cellulase, it releases 4-nitrophenol, a compound that exhibits a yellow color and absorbs strongly at 405 nm, allowing for easy spectrophotometric measurements of cellulase activity levels in various sample types, including bacterial and fungal cultures, environmental samples, and industrial applications.Fórmula:C30H45NO23Pureza:Min. 95%Cor e Forma:PowderPeso molecular:787.67 g/mol4-Methylumbelliferyl 2-deoxy-2-sulfamino-a-D-glucopyranoside sodium salt - Moscerdam™ biochemical purity
CAS:4-Methylumbelliferyl 2-deoxy-2-sulfamino-a-D-glucopyranoside sodium salt (M3A or MU-GlcNS) is a fluorogenic substrate of heparin sulfamidase, used in the diagnosis of Mucopolysaccharidosis III type A (MPS III A). MPS III A, also known as Sanfilippo syndrome type A, is caused by the deficiency of heparan N-sulfatase. Heparin sulfamidase in the assay desulfates MU-GlcNS to 4-methylumbelliferyl 2-amino-2-deoxy-α-D-glucopyranoside (MU-GlcNH2). To release fluorometrically measurable 4-methylumbelliferone (MU) and by-product glucosamine, MU-GlcNH2 is further hydrolysed by an enzyme such as yeast α-glucosidase during a second incubation.Fórmula:C16H18NO10SNaPureza:Min. 95 Area-%Cor e Forma:White PowderPeso molecular:439.37 g/molAldol® 458 phosphate, disodium salt, Biosynth Patent: EP 2427431 and US 8940909
CAS:Aldol® 458 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by phosphatases secreted by bacteria grown in liquid media, yielding yellow color and green fluorescence. Aldol® 458 phosphate can also be used in assays involving phosphatase-coupled antibodies in immunoassay applications (ELISA). Aldol® 458 phosphate can be used under aerobic and anaerobic conditions.Fórmula:C16H12NNa2O6PPureza:Min. 95%Cor e Forma:PowderPeso molecular:391.22 g/mol4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(b-D-galactopyranosyl)-b-D-glucopyranoside
CAS:Chromogenic substrate for fucosyltransferasesFórmula:C20H28N2O13Pureza:Min. 98 Area-%Cor e Forma:White PowderPeso molecular:504.44 g/mol2-Nitrophenyl-beta-D-cellobioside
CAS:Chromogenic substrate for β-D-Cellobiosidase yielding a yellow solution upon cleavage.
Fórmula:C18H25NO13Pureza:Min. 98.0 Area-%Peso molecular:463.4 g/mol4-Methylumbelliferyl a-D-glucosaminide
CAS:4-Methylumbelliferyl alpha-D-glucosaminide is a fluorogenic substrate for alpha-N-acetylglucosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl alpha-D-glucosaminideas a substrate for measuring the alpha-N-acetylglucosaminidase activity is used for Sanfilippo syndrome B and Mucopolysaccharidosis IIIB (MPS IIIB) diagnosis.Fórmula:C16H19NO7Pureza:Min. 90 Area-%Cor e Forma:White PowderPeso molecular:337.32 g/mol5-Bromo-4-chloro-3-indoxyl oleate
CAS:5-Bromo-4-chloro-3-indoxyl oleate is a chromogenic substrate used to detect the specific enzymatic activity of lipase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl oleate is used in lipid metabolism disorder screening.Fórmula:C26H37BrClNO2Pureza:Min. 95%Cor e Forma:PowderPeso molecular:510.93 g/mol6-Chloro-3-indolyl 2-acetamido-2-deoxy-b-D-galactopyranoside
CAS:6-Chloro-3-indolyl 2-acetamido-2-deoxy-b-D-galactopyranoside is a chromogenic substrate for N-acetyl-beta-galactosaminidase. It produces a salmon-pink color after enzymatic cleavage.
Pureza:Min. 95%Cor e Forma:White PowderPeso molecular:370.78 g/mol6'-(Diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl b-D-glucopyranoside
6'-(Diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl b-D-glucopyranoside is a fluorogenic substrate that is used in diagnostic applications. It has been shown to be a useful ligand for the determination of enzyme activity, and it can also be used to detect bacterial culture media contaminants. 6'-(Diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl b-D-glucopyranoside is used as an enzyme substrate in chemiluminescent assays, and with other reagents, it can be used for the detection of environmental contaminants. This product has a CAS number of 55426-02-8 and can be found on the website www.sigmaaldrich.com/catalog/productPureza:Min. 95%Peso molecular:494.49 g/mol5-Bromo-6-chloro-3-indolyl a-D-galactopyranoside
CAS:5-Bromo-6-chloro-3-indolyl a-D-galactopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in glycolipid metabolism studies.Fórmula:C14H15BrClNO6Cor e Forma:White to off-white solid.Peso molecular:408.63 g/molResorufin b-D-xylobioside
Resorufin b-D-xylobioside is a fluorogenic substrate for xylanases useful for xylanase activity assays.Pureza:Min. 95%Cor e Forma:PowderPeso molecular:477.42 g/mol4-Methylumbelliferyl palmitate
CAS:Fluorogenic substrate for esterase yielding a blue fluorescent solution upon cleavage.Fórmula:C26H38O4Pureza:Min. 99 Area-%Peso molecular:414.59 g/molRef: 3D-M-5750
1gA consultar5gA consultar250mgA consultar500mgA consultar2500mgA consultar-Unit-ggA consultar5-Bromo-4-chloro-3-indolyl a-D-mannopyranoside
CAS:5-Bromo-4-chloro-3-indolyl a-D-mannopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-mannosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-mannopyranoside is used in alpha-mannosidosis diagnosis and other lysosomal storage disorder.Fórmula:C14H15BrClNO6Pureza:Min. 95 Area-%Cor e Forma:PowderPeso molecular:408.63 g/mol4-Nitrophenylphosphorylcholine
CAS:Chromogenic substrate used to measure phospholipase C (PLC) activity
Fórmula:C11H17N2O6PPureza:Min. 95 Area-%Cor e Forma:PowderPeso molecular:304.24 g/molAquaSpark® inositol-phosphate, Ramot at Tel-Aviv University Ltd. Patent family WO 2017/130191
Fluorescent reagent for the detection of phospholipids. Previously under code EA179480.
Fórmula:C34H40ClO15PPureza:Min. 90 Area-%Peso molecular:755.1 g/molDL-Luciferin 1-(4,5-dimethoxy-2-nitrophenyl)ethyl ester
CAS:DMNPE-caged form of luciferinFórmula:C21H19N3O7S2Pureza:Min. 95%Cor e Forma:PowderPeso molecular:489.52 g/molAc-Abu-Tle-Leu-Gln-AMC TFA salt
A fluorogenic substrate for detection of enzymatic activity of the SARS-CoV-2 Mpro protease. The fluorophore 7-Amino-4-methylcoumarin (AMC) is linked to the tetrapeptide via a peptide bond at the C-terminal of the glutamine residue. Following the hydrolytic activity of the Mpro protease, the fluorogenic AMC group get released from the substrate, generating a fluorescent signal (Ex 380 nm / Em 455 nm).Fórmula:C33H48N6O8(freebase)Pureza:Min. 95.0 Area-%Peso molecular:656.77 g/mol
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