Substratos enzimáticos

<p>2-(5'-Chloro-2'-hydroxyphenyl)-6-chloro-4(3H)-quinazolinonyl a-D-N-acetylgalactosamine is an inhibitor of bacterial cell wall synthesis. It has been shown to inhibit the enzymatic activity of beta-lactamase, which is an enzyme that breaks down beta-lactam antibiotics. 2-(5'-Chloro-2'-hydroxyphenyl)-6-chloro-4(3H)-quinazolinonyl a-D-N-acetylgalactosamine can be used for the treatment of infections caused by bacteria such as methicillin resistant Staphylococcus aureus. This compound has been shown to have low toxicity and does not cause any significant side effects in animals.</p>

<p>AquaSpark® Alkaline Phosphatase Substrate is a stand-alone reagent - no additives such as enhancers or detergents are required to start a strong light reaction. AquaSpark® Alkaline Phosphatase Substrate is superior to other luminogenic alkaline phosphatase substrates as it shows significantly higher signal intensities and very low background.Application Examples:</p>

<p>6-Chloro-1H-indol-3-yl b-D-glucuronide is a chemiluminescence substrate that can be used in immunoassays and other biochemical analyses. 6CIG is a highly purified, high quality product with a CAS No. 138182-19-1. This product has been tested for bacterial contamination and found to be free of detectable levels of mycoplasma, yeast, mold, or other microbial agents. It has also been tested for its ability to support the growth of various types of cells and found to be suitable for cell culture media. This chemical has been shown to produce an intense blue chemiluminescent reaction when reacted with horseradish peroxidase enzyme and hydrogen peroxide.</p>

<p>4-Methylumbelliferyl 2-acetamido-2-deoxy-3-O-(alpha-L-fucopyranosyl)-beta-D-glucopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-3-O-(alpha-L-fucopyranosyl)-beta-D-glucopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for fucosidosis diagnosis (deficiency of the enzyme alpha-L-fucosidase) and lysosomal storage disorder screening.</p>

<p>Resorufin phosphate disodium is a fluorogenic substrate for phosphatase exhibiting a red color after cleavage that can be measured at ~ 585nm.</p>

<p>L-2-Chloro-4-nitrophenyl 2-acetamido-2-deoxy-a-D-glucopyranoside is a chemiluminescent substrate for the detection of aminoglycoside antibiotics. It can be used to determine the concentration of aminoglycosides in blood, urine, and serum. The chemiluminescence reaction is dependent on the presence of oxygen. This product has been shown to have a high quality and purity with environmental testing and staining properties. L-2-Chloro-4-nitrophenyl 2-acetamido-2-deoxy--a--D--glucopyranoside has also been shown to bind ligands such as porphyrins, bilirubin, or hemin with bioluminescence or chromogenic substrates in diagnostics or culture media for food testing. L--2--Chloro--4--</p>

<p>4-Nitrophenyl 3,4,6-tri-O-acetyl-b-D-galactopyranoside is a nucleic acid molecule that has been synthesized by recombinant expression in Corynebacterium glutamicum. It is a photosynthetic molecule and can be used to study the molecular basis of photosynthesis. The 4-nitrophenyl group on the sugar moiety confers resistance to degradation by nucleases. 4NP3,4,6GalAc has been shown to be capable of binding to proteins and polypeptides that are important for photosynthesis. This compound also has marginalizing effects on C. glutamicum strains that are used as expression vectors for recombinant protein production.</p>

<p>4-Methylumbelliferyl 2-azido-2-deoxy-4,6-O-phenylmethylene-a-D-galactopyranoside is a fluorogenic substrate that reacts with beta D galactosidase to produce light. This product is useful for diagnostics, conjugate production, and other applications. 4MUF 2 -Gal provides a convenient means of detecting beta D galactosidase activity in cell culture media and can be used as a chromogenic substrate for the detection of enzymes involved in glycosylation reactions. It is also used to detect staining by bioluminescence. The compound can be used in food testing and environmental testing to measure the presence of beta D galactosidase.</p>

<p>2-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-galactopyranoside is a chemiluminescent substrate for the detection of beta galactosidase. It is a white to light yellow powder with a molecular weight of 564.87 and an assay of ≥ 98% by HPLC. This product has been shown to be stable when stored at 4°C for up to three months.</p>

<p>4-Methylumbelliferyl 3,4,6-tri-O-acetyl-2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)-b-D-galactopyranoside is a fluorogenic substrate that is used for the detection of β D -galactosidase. β D -Galactosidase is an enzyme that breaks down lactose in milk and is used as a diagnostic tool for identifying lactose intolerance. This substrate can be used as a conjugate in immunoassays to detect antibodies against the enzyme or it can be used to measure the activity of the enzyme in food samples. It also can be used to measure α1,3 galactosyltransferase activity in colorectal cancer cells or bacteria.</p>

<p>4-Nitrophenyl 3,6-di-O-(a-D-mannopyranosyl)-b-D-mannopyranoside is a chemiluminescent substrate that is used in the detection of enzymes. It is also used to measure the activity of beta-glucosidase, alpha-galactosidase, and alkaline phosphatase. The product has been tested for environmental sensitivity and has not shown any effects on bacterial growth. 4-Nitrophenyl 3,6-di-O-(a-D-mannopyranosyl)-b-D-mannopyranoside can be used as a high quality stain for histological sections and immunohistochemical studies. This product has been tested for staining properties with different dyes and has shown no interference with the staining process.</p>

<p>4-Nitrophenyl 2-acetamido-4-O-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranosyl)-3,6-di-O -acetyl2 deoxy b D glucopyranosid e is a chemiluminescence substrate that reacts with hydrogen peroxide to produce light. It is used in diagnostics for the detection of bacterial and fungal cultures. The reaction between 4NPAA and hydrogen peroxide generates light at 540 nm. This product has been tested for purity and quality using various methods including culture media, environmental testing, bioluminescence, staining, fluorogenic substrate, enzyme substrate, food testing and conjugate.</p>

<p>Phenylethyl 2-acetamido-2-deoxy-b-D-glucopyranoside can be used to inhibit the growth of bacteria, yeast, and fungi. It is a yellow powder that has been shown to cause regression in tumor xenografts in mice. Phenylethyl 2-acetamido-2-deoxy-b-D-glucopyranoside inhibits cellular proliferation by blocking the synthesis of proteins and nucleic acids. This compound also has been shown to have an effect on the environment because it inhibits the growth of phytoplankton. Phenylethyl 2-acetamido-2-deoxy-b-D-glucopyranoside was found to disrupt bacterial gene expression profiles, which may be due to its ability to inhibit protein synthesis at the ribosome level.</p>

<p>Luciferin-caprylate is a substrate for chemiluminescence, which is the light produced by a chemical reaction. It has been shown to have high quality and environmental testing as well as being a ligand for bioluminescence. Luciferin-caprylate can be used for diagnostics in the areas of culture media, food testing, and enzyme substrate. This compound can also be used as a fluorogenic substrate or conjugate.</p>

<p>2-Naphthyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is a fluorogenic substrate that is used in food testing as well as environmental testing. It has been shown to be a ligand for the enzyme beta-galactosidase. This product is also a chemiluminescent and bioluminescent substrate that can be used in diagnostics and culture media.</p>

<p>5-Bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt - micronized is a chemiluminescent substrate used in diagnostic assays. It is an enzyme substrate that is commonly used to detect peroxidase activity and has been shown to be effective in detecting various types of bacteria. This product is a high quality chemical with a purity of at least 98% and has been tested for environmental safety. 5-Bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt - micronized can be used as a fluorogenic substrate for the detection of proteases and aminopeptidases.</p>

<p>1-Naphthyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is a fluorogenic substrate for bacterial beta D glucosidase. It is used for measuring the enzyme activity in different types of culture media and in the detection of bacterial contamination in food and environmental samples. This product can be used to detect the presence of beta D glucosidase by measuring luminescence with a luminometer. 1NAAG can also be used as a chromogenic substrate or to conjugate with antibodies for use in immunoassays.br&gt;</p>

<p>4-Nitrophenyl b-D-thiomannopyranoside acts as a substrate for β-mannosidase and thioglycoligase. Upon enzymatic cleavage, these substrates release 4-nitrophenol, a yellow chromophore that can be detected spectrophotometrically at 405 nm, facilitating quantitative measurements of enzymatic activity. Consequently, these substrates are widely used in biochemical research, clinical diagnostics, and drug discovery for evaluating the function of specific enzymes in various biological processes.</p>

<p>4-Methylumbelliferyl N,N',N'',N'''-tetraacetyl-beta-D-chitotetraoside is a fluorogenic substrate for chitinase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl N,N',N'',N'''-tetraacetyl-beta-D-chitotetraosideas a substrate for measuring the chitinase activity is used for fungal infection detection and Gaucher disease screening.</p>

<p>4-Nitrophenyl 3,4-O-isopropylidene-a-D-galactopyranoside is a chemiluminescent substrate for detecting beta-galactosidase activity in bacteria. This product is a high purity, high quality compound that can be used as a culture media and environmental testing agent. It has been shown to be effective in diagnostics, chromogenic substrate, and enzyme substrate reactions. 4NPPG has been shown to be a fluorogenic substrate for beta-galactosidase assay and can be used as food testing agent with conjugate.</p>

<p>BCIP is a dye that can be used as a substrate in a fluorometric assay to detect the presence of effector proteins. It is also used in analytical chemistry to detect phosphate, and as a reagent in diagnostic tests for mucin gene expression. BCIP has been shown to have transcriptional regulatory effects on genes encoding for proteins involved in antibody production. BCIP has been shown to act as an effector protein, which influences the amount of mRNA produced by the gene encoding for tissue culture cells. BCIP can also be used as a model system to study the regulation of transcription in vitro.<br>A hybridization technique using BCIP with DNA-dependent RNA polymerase II has been demonstrated and applied to analyze the mechanism of transcriptional control at the level of individual genes. The use of this technique allows for analysis at a single gene level and may allow for better understanding of transcriptional regulation.</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(a-D-galactopyranosyl)-a-D-galactopyranoside is a fluorescent substrate that can be used in chemiluminescent assays. It is a high purity, high quality chemical that has been tested for culture media, environmental testing and bioluminescence. This product is an excellent choice for use in diagnostics, chromogenic substrates, enzyme substrates and food testing.</p>

<p>2-Nitrophenyl 2,3-di-O-acetyl-4-O-triethylsilyl-b-D-xylopyranoside is a chromogenic substrate for enzmatic assays to measure xylosidase activity. It is a derivative of xylose that can be hydrolyzed by xylosidase to release the yellow-colored 2-nitrophenyl group, which can be detected spectrophotometrically. By modifying the xylose molecule with acetyl and triethylsilyl groups, the enzyme substrate becomes more stable and resistant to degradation. This substrate is commonly used in the study of plant biomass degradation and in the detection of xylosidase activity in microorganisms.</p>

<p>2-Nitrophenyl-b-D-glucuronide is a chromogenic substrate used to detect the activity of beta-glucuronidase in various biological samples. Upon hydrolysis by the enzyme, the substrate releases 2-nitrophenol, which can be spectrophotometrically measured at 405 nm. The substrate is commonly used in clinical assays to evaluate liver and kidney function, as well as in drug metabolism studies. It is also used in environmental monitoring to assess the presence of microbial activity in soil and water samples. Overall, 2-Nitrophenyl-b-D-glucuronide is a widely used substrate with excellent sensitivity and specificity, making it a valuable tool in enzymology research and diagnostic testing.</p>

<p>Resorufin maltotriose is an alpha-amylase substrate that releases the dye resorufin with red emission (lmax=585 nm) after enzyme reaction.</p>

<p>4-Nitrophenyl 2-O-(α-L-fucopyranosyl)-β-D-galactopyranoside is a chromogenic enzymatic substrate for the detection and quantification of glycosidases, such as fucosidases and galactosidases. Upon enzymatic cleavage, the substrate releases the p-nitrophenol chromophore, which can be quantified via colorimetric analysis. This pNP-conjugated compound enables the rapid, sensitive, and high-throughput detection of enzyme activity in various applications, including enzymatic assays, inhibitor screening, and mechanistic studies. Its precise specificity for α-L-fucopyranosyl and β-D-galactopyranoside moieties makes it an excellent tool for advancing research in glycobiology and related fields.</p>

<p>2-Aminophthalylhydrazido 2-acetamido-2-deoxy-b-D-glucopyranoside is a chromogenic substrate that is used as a diagnostic in biological research. It reacts with enzymes to form fluorescent products, and can be used to detect the presence of bacteria and fungi. This product has a CAS number of 128879-80-1 and can be used for food testing, environmental testing, or bioluminescence. It is an important component in the development of new antibiotics.</p>

<p>5-Octanoylaminofluoroscein di-b-D-glactopyranoside is a chemiluminescent substrate that can be used for the detection of peroxidase activity. It is a bifunctional substrate, which means it has two reactive groups: one at the 5 position and one at the 8 position. The 5-octanoylaminofluorescein reacts with hydrogen peroxide in a 1:1 ratio, emitting light at 490 nm. The 8-position amine reacts with 2,2'-azino-bis(3-ethylbenzthiazoline)6 sulphonic acid (ABTS) to produce an orange color product that acts as a chromogen. The substrate can be used in diagnostics, food testing, and enzyme substrates.</p>

<p>2-Nitrophenyl 2,3,4,6-tetra-O-acetyl-b-D-galactopyranoside is a fluorescent substrate for β-galactosidase. It is used in food testing, environmental testing and diagnostics. This substrate is also used to detect the presence of β-galactosidase activity in cells and tissues.<br>2NPTABG provides a fluorescence signal that can be detected with an ultraviolet light or a luminometer. The luminescence intensity decreases when it reacts with β-galactosidase enzyme.<br>This product has a CAS number of 3053-17-6 and is available at high purity levels.</p>

<p>Phenylethyl b-D-thioglucopyranoside is a modification of the sugar molecule. It is an oligosaccharide that can be synthesized from a complex carbohydrate. Phenylethyl b-D-thioglucopyranoside is a high purity product that can be custom synthesized with high purity and CAS No. It also has methylation, glycosylation, and polysaccharide properties. The product's chemical structure can be modified in order to change the properties of this substance. This product is fluorinated or saccharified.</p>

<p>Chemiluminescent substrate for beta-D-glucosidase</p>

<p>H-Pro-Arg-4mbna is a chromogenic substrate that is specifically cleaved by trypsin, a serine protease involved in protein digestion, and thrombin, that cleave peptide bonds. Upon hydrolysis, the bond between the proline and arginine is cleaved, releasing 4MβNA. This leads to a colorimetric change that can be detected spectrophotometrically. This substrate is commonly used in the study of trypsin activity, as well as in the development of assays for the diagnosis and monitoring of pancreatic diseases, such as pancreatitis and cystic fibrosis, which are associated with trypsin dysfunction.</p>

<p>4-Methylumbelliferyl-beta-D-cellobioside heptaacetate is a fluorogenic substrate for beta-D-glucosidase and can be used to detect this enzyme in the presence of other glycosidases. It is also used as a chromogenic substrate for beta-galactosidase, alpha-mannosidase, alpha-fucosidase, and alpha-N-acetylgalactosaminidase. 4MMB Cellobiose Haptaacetate is a conjugate that can be used to measure the activity of beta-glucuronidase and beta-xylosidase. This product is suitable for use in diagnostics, ligands, food testing, environmental testing, and culture media.</p>

<p>2,4-Dinitrophenyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a fluorogenic substrate for use in diagnostic testing. It reacts with the enzyme β -galactosidase to produce a blue color. This product is used as an indicator of β -galactosidase activity in bacterial cultures or as an indicator of the presence of this enzyme in food or other materials. It can be used to detect and measure the level of β -galactosidase in a sample. The reaction can be monitored by measuring light emission at 450 nm.<br>2,4-Dinitrophenyl 2 acetamido 2 deoxy b D glucopyranoside has a CAS number of 47868-87-0 and is available in high purity (&gt;97%).</p>

<p>2-Nitrophenyl 2-acetamido-2-deoxy-a-D-galactopyranoside is a fluorogenic substrate that is used in enzyme assays. It can be used as a chromogenic substrate or conjugate with an enzyme to detect the presence of glucose.</p>

<p>4-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is an extracellular nucleic acid molecule that is produced by the bacterium Corynebacterium glutamicum. This compound has been shown to inhibit cancer cells by blocking the transport of glucose through the cell membrane and may be a potential drug target for cancer treatment. 4NPTAPG has also been shown to have diagnostic properties and can identify corynebacterium glutamicum strains. It is a fluorescent molecule that can be used to detect the presence of this bacterium in culture media.</p>

<p>3-Indolyl b-D-cellotetraoside is a chemiluminescent substrate that is used as an indicator in the detection of aminoglycosides. The ligand is a cell wall component that has been shown to bind specifically to the active site of bacterial β-lactamases, which are enzymes that catalyze the hydrolysis of β-lactam antibiotics. 3-Indolyl b-D-cellotetraoside can be used for bacterial identification and enumeration, food testing, and enzyme substrate for diagnostics. It fluoresces in response to electron excitation with a wavelength of about 590 nm.</p>

<p>Substrate for alkaline phosphatase</p>

<p>4-Nitrophenyl 2-O-(α-D-glucopyranosyl)-α-D-glucopyranoside is a specialized chromogenic pNP substrate featuring dual glucose-linked moieties, designed for accurate detection and quantification of specific glycosidase enzymes. Upon cleavage by target enzymes, this substrate releases 4-Nitrophenol, which can be easily monitored spectrophotometrically as a yellow color change. This advanced substrate offers enhanced specificity and sensitivity in enzyme assays, facilitating cutting-edge research in biochemistry, enzymology, and other related fields.</p>

<p>4-Methylumbelliferyl 4,6-O-benzylidene-b-D-galactopyranoside is a fluorogenic substrate that is used in the detection of galactosidases. It has been shown to be a potent inhibitor of these enzymes and can be used to study the activity of various galactosidases. This substrate is used in the measurement of carbohydrate metabolism, including glycolysis and glycogenolysis. 4-Methylumbelliferyl 4,6-O-benzylidene-b-D-galactopyranoside has been shown to produce chemiluminescence when reacted with horseradish peroxidase and hydrogen peroxide. It also produces bioluminescence when exposed to luciferase and adenosine triphosphate (ATP).</p>

<p>4-Methylumbelliferyl alpha-L-idopyranoside is a fluorogenic substrate for alpha-L-iduronidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl alpha-L-idopyranosideas a substrate for measuring the alpha-L-iduronidase activity is used for Mucopolysaccharidosis I diagnosis and Hurler syndrome screening.</p>

<p>4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-b-D-glucopyranoside enzyme substrate is a versatile and reliable reagent for measuring glucosidase activity. It is an ideal substrate for use in assays to detect β-glucosidase activity in a variety of applications, including for in vitro and in vivo studies, and it has been widely used in the pharmaceutical and biotechnology industries. This chromogenic pNP enzyme substrate is designed for effective and precise analysis with good sensitivity and low background signals.</p>

<p>2-Nitrophenyl 2-acetamido-2-deoxy-b-D-galactopyranoside is a carboxymethyl derivative of the natural substrate of β-galactosidase, which is used as a substrate for the production of recombinant proteins in the corynebacterium glutamicum. The enzyme β-galactosidase hydrolyzes this substrate to produce an acid molecule and a sugar molecule. This product can be used in biotechnological processes for the production of recombinant proteins or other bioactive molecules. It can also be used in the isolation of nucleic acid from bacteria or fungi, or to study bacterial membrane transport systems. 2NPGAG can be expressed as a recombinant protein with expression vectors such as pET21a and pET24a. It has been shown that this product inhibits transport through membranes by binding to cellulose, which may be useful for studies on cellulose biosynthesis</p>

<p>Aldol acetate is a chromogenic substrate that is used in food testing and diagnostics. Aldol acetate can be used in the detection of aminopeptidase and dipeptidyl peptidase activities, as well as in the determination of urinary proteins. Aldol acetate can also be used to detect anaerobic bacteria. This compound is conjugated with various ligands for use in immunoassays, such as beta-galactosidase, beta-glucuronidase, alpha-naphthyl phosphate ester, and phenyl phosphate ester. This product has been shown to be useful for chemiluminescent assays of DNA polymerases and reverse transcriptases in environmental samples. It has also been shown to be useful for bioluminescent assays of DNA polymerases and reverse transcriptases in environmental samples.</p>

<p>2-Nitrophenyl-2,3,4-tri-O-acetyl-b-D-glucuronide methyl ester is a tri-O-acetylated glucuronide substrate commonly used in chromogenic assays to detect the activity of beta-glucuronidase. Upon hydrolysis by the enzyme, the substrate releases 2-nitrophenol, which can be spectrophotometrically. The substrate is widely used in diagnostic tests to assess bacterial and fungal infections, as well as in drug metabolism studies.</p>

<p>4-Methylumbelliferyl 3,4,6-tri-O-acetyl-2-deoxy-2-trifluoroacetamido-a-D-glucopyranoside (4MUAA) is a fluorescent dye that is used for the detection of envelopment in cells. This dye binds to cell membranes and can be detected with a fluorescence microscope. 4MUAA can be used to determine the efficiency of an experimental methodology or algorithm by measuring the change in fluorescence intensity as a result of experimental treatment. The use of 4MUAA has been shown to be effective in determining whether or not there is an association between nationwide environmental pollution and cancer rates in Japan.</p>

<p>Aldol 491 acetate is a food and environmental testing chemical, which can be used for the detection of bacteria. Aldol 491 acetate provides a chromogenic substrate for the detection of enzymes and can be used as a substrate in enzyme-linked immunosorbent assay (ELISA) procedures. Aldol 491 acetate also has chemiluminescent properties and can be used as a fluorogenic substrate.</p>

<p>Chromogenic substrate targeting amylase</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-6-O-trityl-b-D-glucopyranoside offers unmatched versatility as a chromogenic pNP substrate for detecting b-D-glucopyranosidase activity. The substrate is specifically designed to produce a yellow 4-nitrophenol product upon enzyme hydrolysis, which can be measured using absorbance spectroscopy. The unique tritylated glucopyranoside group ensures improved compound stability and selectivity while providing ease of handling. This versatile substrate is ideal for a broad range of biochemical, research, and diagnostic applications.</p>

<p>Cesium carbonate is a compound that has various applications in different fields. It is commonly used as an aldehyde scavenger in organic synthesis reactions. Cesium carbonate can also interact with cerebrosides, which are important components of the nervous system, and may have potential therapeutic effects on certain neurological disorders.</p>