6-Chloro-3-indoxyl-beta-D-galactopyranoside

CAS: 138182-21-5

Ref. 3D-C-5000

Informação sobre produto

• Nome: 6-Chloro-3-indoxyl-beta-D-galactopyranoside
• Sinónimos: Salmon-beta-D-galactosideEC05439, 6-Chloro-1H-indol-3-yl β-<span class="text-smallcaps">D</span>-galactopyranoside, 6-Chloro-3-indolyl--D-galactopyranoside, 6-chloro-1H-indol-1-yl beta-D-galactopyranoside, 6-chloro-1H-indol-3-yl β-D-galactopyranoside, ChloroindolylDgalactopyranoside, Red-Gal(r) 6-Chloro-3-indolyl-!-D-galactoside, Salmon Galactoside, β-<span class="text-smallcaps">D</span>-Galactopyranoside, 6-chloro-1H-indol-3-yl, β-D-Galactopyranoside, 6-chloro-1H-indol-3-yl

Descrição

6-Chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase that yields a salmon or rose-coloured precipitate and hence is also known as Salmon-Gal. It can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside (IPTG) as an alternative to the chromogenic substrate 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-Gal) for detection of beta-galactosidase activity in bacterial colonies in colorimetric assays. Where beta-galactosidase expressed from a reporter gene in molecular biology experiments, recombinant colonies on growth plates are salmon-coloured while non-recombinants appear as white. Use of nitroblue tetrazolium salt (NBT) with Salmon-Gal may provide faster results, yielding a clear, purple precipitate, than for Salmon-Gal alone. In conjunction with 5-bromo-4-chloro-3-indolyl-beta-D-glucoside (X-Glc), Salmon-Gal is useful for simultaneous detection of GUS and Lac activities in the same medium.

• Marca: Biosynth

Propriedades químicas

• Peso molecular: 329.74 g/mol
• Fórmula: C₁₄H₁₆ClNO₆
• Pureza: Min. 98.0 Area-%