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2-Fluoro-N-(3-Nitrophenyl)Acetamide (2FNA) is a chemical that is used in analytical methods to measure sodium fluoroacetate in water samples. 2FNA is the reagent that mediates the reaction between sodium fluoroacetate and an extractant in a liquid chromatography column. The 2FNA interacts with a molecule of sodium fluoroacetate to form an ionized molecule of 2-fluoro-N-(3-nitrophenyl) acetamide. This ionized molecule can be analyzed by chemical ionization or electrospray mass spectrometry. The calibration of this method was performed using stable isotopes and matrix matched calibration, which ensures accurate results. Recoveries were found to be high for both anionic and cationic compounds, making it possible for this method to be utilized for many different applications.
2FNA is also used as a reagent in other analytical methods such as
