Mouse HSF1(Heat Shock Transcription Factor 1) ELISA Kit

Ref. EK-ELK0185

Informação sobre produto

• Nome: Mouse HSF1(Heat Shock Transcription Factor 1) ELISA Kit
• Sinónimos: HSTF1; Heat shock factor protein 1

Descrição

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HSF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HSF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HSF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HSF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marca: ELK Biotechnology