Human PDK4(Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit

Ref. EK-ELK0321

Informação sobre produto

• Nome: Human PDK4(Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit
• Sinónimos: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; Pyruvate Dehydrogenase Kinase 4

Descrição

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDK4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDK4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDK4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDK4 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marca: ELK Biotechnology