EasyStep Human VCAM1(Vascular Cell Adhesion Molecule 1) ELISA Kit

Ref. EK-ELK043ES

Informação sobre produto

• Nome: EasyStep Human VCAM1(Vascular Cell Adhesion Molecule 1) ELISA Kit
• Sinónimos: CD106; INCAM-100; L1CAM

Descrição

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VCAM1, and the Human VCAM1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VCAM1. After TMB substrate solution is added, only those wells that contain Human VCAM1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VCAM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marca: ELK Biotechnology