Rat HSPD1(Heat Shock 60kD Protein 1, Chaperonin) ELISA Kit

Ref. EK-ELK1614

Informação sobre produto

• Nome: Rat HSPD1(Heat Shock 60kD Protein 1, Chaperonin) ELISA Kit
• Sinónimos: HSP60; GROEL; CPN60; HuCHA60; Heat Shock Protein 60; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

Descrição

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HSPD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HSPD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HSPD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HSPD1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marca: ELK Biotechnology