Human TIEG1(TGF β Inducible Early Response Gene 1) ELISA Kit

Ref. EK-ELK1827

Informação sobre produto

• Nome: Human TIEG1(TGF β Inducible Early Response Gene 1) ELISA Kit
• Sinónimos: KLF10; EGRA; TIEG; Krueppel-Like Factor 10; TGFB Inducible Early Growth Response; EGR-alpha; Transforming growth factor-beta-inducible early growth response protein 1

Descrição

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TIEG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TIEG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TIEG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TIEG1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marca: ELK Biotechnology