Rat PK(Pyruvate Kinase, Liver And RBC) ELISA Kit
Ref. EK-ELK2803
48T | 404,00 € | ||
96T | 577,00 € |
Informação sobre produto
- PKLR; PyK; PK1; PKL; Pyruvate Kinase Isozymes R/L; Glutamic--pyruvic transaminase 1; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PK in the samples is then determined by comparing the OD of the samples to the standard curve.
Propriedades químicas
Consulta técnica sobre: EK-ELK2803 Rat PK(Pyruvate Kinase, Liver And RBC) ELISA Kit
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