Human OB(Obestatin) ELISA Kit

Ref. EK-ELK5480

Informação sobre produto

• Nome: Human OB(Obestatin) ELISA Kit
• Sinónimos: Obestatin

Descrição

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OB in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marca: ELK Biotechnology