Rat IL-2sRa/CD25(Soluble Interleukin-2 Receptor α chain) ELISA Kit
Ref. EK-ELK5694
| 48T | 402,00 € | ||
| 96T | 575,00 € |
Informação sobre produto
- IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.
Propriedades químicas
Consulta técnica sobre: EK-ELK5694 Rat IL-2sRa/CD25(Soluble Interleukin-2 Receptor α chain) ELISA Kit
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