Human WNT3A(Wingless Type MMTV Integration Site Family, Member 3A) ELISA Kit

Ref. EK-ELK7541

Informação sobre produto

• Nome: Human WNT3A(Wingless Type MMTV Integration Site Family, Member 3A) ELISA Kit
• Sinónimos: Protein Wnt 3a Precursor; Protein Wnt-3a; Wnt 3a; wnt3a; WNT3A protein

Descrição

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WNT3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WNT3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WNT3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human WNT3A in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marca: ELK Biotechnology