5-Me-THF(5-Methyltetrahydrofolate) ELISA Kit

Ref. EK-ELK8059

Informação sobre produto

• Nome: 5-Me-THF(5-Methyltetrahydrofolate) ELISA Kit
• Sinónimos: 5-Me-THFA; 5-Me-H4FA; 5-Me-THF; 5-Me-H4F; Levomefolic Acid; Metafolin; 5-Methyltetrahydrofolic Acid; L-Methylfolate

Descrição

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Me-THF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Me-THF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Me-THF in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marca: ELK Biotechnology