Human HVD3(25-Hydroxyvitamin D3) ELISA Kit
Ref. EK-ELK8625
48T | 333,00 € | ||
96T | 472,00 € |
Informação sobre produto
- 25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HVD3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Propriedades químicas
Consulta técnica sobre: EK-ELK8625 Human HVD3(25-Hydroxyvitamin D3) ELISA Kit
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