Mouse IL-27(Interleukin 27) ELISA Kit
Ref. EK-ELK9612
| 48T | 403,00 € | ||
| 96T | 575,00 € |
Informação sobre produto
- IL-27; IL-27A; IL27p28; IL30; MGC71873; p28;
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-27 in the samples is then determined by comparing the OD of the samples to the standard curve.
Propriedades químicas
Consulta técnica sobre: EK-ELK9612 Mouse IL-27(Interleukin 27) ELISA Kit
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