CAS 125328-76-9: 5-BROMO-4-CHLORO-3-INDOXYL-1-ACETATE
CAS 125328-76-9: 5-BROMO-4-CHLORO-3-INDOXYL-1-ACETATE
Description:5-Bromo-4-chloro-3-indoxyl-1-acetate is a synthetic organic compound primarily used as a chromogenic substrate in biochemical assays, particularly in the detection of β-galactosidase activity. This compound features a complex indole structure, which contributes to its unique reactivity and colorimetric properties. Upon enzymatic hydrolysis, it produces a colored product, making it valuable in molecular biology and microbiology for visualizing enzyme activity. The presence of bromine and chlorine substituents enhances its chemical stability and reactivity, allowing for specific interactions in biological systems. Additionally, the acetate group serves as a protective moiety, which is cleaved during the enzymatic reaction, further facilitating the detection process. Overall, 5-bromo-4-chloro-3-indoxyl-1-acetate is an important tool in research and diagnostic applications, providing a reliable method for studying enzyme kinetics and cellular processes.
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