Altération de l'ADN/réparation de l'ADN

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PARP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PARP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PARP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PARP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Flumequine (R-802) is a broad-spectrum antibiotic targeting DNA gyrase and topoisomerase IV in Gram-positive and Gram-negative bacteria.

<p>Oxaquin TEA (MCB-3837 TEA), a precursor compound to MCB3681, is a DNA topoisomerase inhibitor that can be used to study Clostridium difficile infections and</p>

Elimusertib hydrochloride(1876467-74-1 free base) (BAY-1895344 hydrochloride) is a effective, orally available and selective ATR inhibitor with anti-tumor

<p>Carboplatin (JM-8) is a cisplatin derivative, a DNA synthesis inhibitor.</p>

Ciprofloxacin monohydrochloride (Bay-09867 hydrochloride) is a broad-spectrum antimicrobial carboxyfluoroquinoline.

Levetiracetam (SIB-S1) is a relatively unique anticonvulsant that is typically used in combination with other antiepileptic medications for partial onset

Ceramides Mixture: endogenous, regulates cell cycle, growth, and telomerase activity, with hydroxy/non-hydroxy fatty acids.

<p>Niraparib R-enantiomer (MK 4827 R-enantiomer) is an inhibitor of PARP1(IC50 of 2.4 nM).</p>

FEN1-IN-1 (LNT-1), a FEN1 active site inhibitor, partly works by coordinating Mg2+ ions.

Procarbazine hydrochloride (NSC-77213 HCl) is the hydrochloride salt of a methylhydrazine derivative with antineoplastic and mutagenic activities.

Nalidixic acid (NSC-82174), a synthetic antimicrobial, targets bacterial DNA gyrase A, with a narrow bactericidal range.

AES-135 is a potent HDAC inhibitor, inhibits HDAC3, HDAC6, HDAC11 (IC50s: 654, 190, and 636 nM) with anti-tumor activity.

LTURM34 is an inhibitor of DNA-PK with IC50 of 34 nM.

Balofloxacin, a quinolone antibiotic, can inhibit the synthesis of bacterial DNA by interfering with the DNA gyrase.

STL127705 inhibits Ku 70/80 protein & DNA-PKCS kinase, IC50s: 3.5 μM & 2.5 μM.

Arhalofenate (JNJ 39659100) is a selective partial agonist of peroxisome proliferator-activated receptor PPARγ. It is used for the treatment of type 2 diabetes.

Cisplatin (CDDP) is a DNA cross-linking agent.

Tallimustine HCl, an AT-specific alkylating agent, treats leukemia in immunodeficiency mouse models.

Norfloxacin (MK-0366)(Norxacin) is a broad-spectrum antibiotic that is active against both Gram-positive and Gram-negative bacteria.