Substrati fluorogenici

Maltotetraose 2-aminobenzamide is a chemiluminescent substrate that emits light in the presence of certain oxidizing agents. It provides high quality and environmental testing, as well as staining and ligand capability. Maltotetraose 2-aminobenzamide is used in diagnostics to identify bacteria and other microorganisms. Maltotetraose 2-aminobenzamide can be used for the detection of bacterial colonies on agar plates using a fluorogenic substrate, or for the detection of fungi using a chromogenic substrate. Maltotetraose 2-aminobenzamide is also used as an enzyme substrate in food testing or conjugate with enzymes for bioluminescence.

6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-cellotetraoside is a fluorescent substrate that is used to measure the activity of the enzyme beta-glucuronidase. It is a chemiluminescent compound that emits light when hydrolyzed by an enzyme. 6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-cellotetraoside has been shown to be a high quality and environmentally friendly product, as it does not cause any significant change in pH or turbidity in water samples. It has also been shown to be nontoxic and nonmutagenic at concentrations up to 400 ppm. This product can be used for a variety of purposes, including diagnostic tests for bacterial infections or food testing.

4-Methylumbelliferyl 3-O-(α-L-fucopyranosyl)-β-D-galactopyranoside is an organic compound that is used as a radioactive marker for DNA. It can be synthesized in the laboratory and its use allows for the tracking of DNA molecules. The production of this marker is achieved by recombinant expression of the gene encoding the enzyme β-D-galactosidase, which hydrolyzes 4-methylumbelliferyl β-D-galactopyranoside to release 4-methylumbelliferone and α-L-fucopyranose. The enzyme uses UDP glucose as a cofactor to catalyse the reaction. This product has been isolated from Corynebacterium glutamicum, with recombinant expression vectors used to produce it in host cells such as Escherichia coli or Corynebacterium glutamicum. It has been found that

Resorufin benzyl ether is a chemical compound that can be used as a substrate for cytochrome P450. It has been shown to inhibit the activity of epoxide hydrolase, an enzyme involved in the metabolism of endoperoxides. Resorufin benzyl ether has also been found to inhibit cytochrome P450 enzymes, which are responsible for oxidative metabolism of xenobiotics. This compound has been shown to have a high affinity for mouse monoclonal antibody and is used in immunoassays to detect mouse antibodies. Resorufin benzyl ether is used in biochemical assays and polymerase chain reactions (PCRs) as a fluorescence marker. In addition, this compound is used in model systems to study enzyme activities such as recombinant cytochrome p450 and protein oxidation by reactive oxygen species (ROS).

4-Methylumbelliferyl alpha-L-idopyranoside is a fluorogenic substrate for alpha-L-iduronidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl alpha-L-idopyranosideas a substrate for measuring the alpha-L-iduronidase activity is used for Mucopolysaccharidosis I diagnosis and Hurler syndrome screening.

5-Octanoylaminofluoroscein di-b-D-glactopyranoside is a chemiluminescent substrate that can be used for the detection of peroxidase activity. It is a bifunctional substrate, which means it has two reactive groups: one at the 5 position and one at the 8 position. The 5-octanoylaminofluorescein reacts with hydrogen peroxide in a 1:1 ratio, emitting light at 490 nm. The 8-position amine reacts with 2,2'-azino-bis(3-ethylbenzthiazoline)6 sulphonic acid (ABTS) to produce an orange color product that acts as a chromogen. The substrate can be used in diagnostics, food testing, and enzyme substrates.

4-Methylumbelliferyl 2,3,4-tri-O-benzoyl-b-D-galactopyranoside-6-sulfate is a fluorogenic substrate for β-galactosidase and a chromogenic substrate for β-glucuronidase. It is used in food testing, environmental testing, and diagnostics.

6-Chloro-7-hydroxy-4-(trifluoromethyl)coumarin is a fungicide that inhibits the growth of fungi by interfering with their DNA synthesis. It does this by inhibiting the activity of two enzymes: the fungal enzyme oxime and the enzyme cinerea, which is found in many fungi. 6-Chloro-7-hydroxy-4-(trifluoromethyl)coumarin has been shown to be effective against Alternaria and Botrytis cinerea. The compound also has an antifungal effect against other types of fungi, such as Penicillium roqueforti, when used in combination with a solvent such as ether.

6,8-Difluoro-4-methylumbelliferyl 2-acetamido-3-O-(b-D-galactopyranosyl)-a-D-galactopyranoside is a chemiluminescent substrate that reacts with oxygen in the presence of luciferase to produce light. This product is an excellent replacement for luminol, especially in applications where aqueous solvents are not suitable. 6,8-Difluoro-4-methylumbelliferyl 2-acetamido-3-O-(b-D-galactopyranosyl)-a-D-galactopyranoside has been extensively tested and shown to be nonhazardous in a variety of different culture media. It is also nonhazardous to the environment, as it can be easily degraded by microorganisms.