Substrati enzimatici

Benzocaine fructoside is a synthetic carbohydrate that is used as a local anesthetic. It is synthesized from benzocaine to produce a glycosylated form of the drug. This compound has been modified to include fluorine atoms, and it has been methylated with primary amines. The modification of this compound has produced a high-purity product that can be used for click chemistry.

6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-cellotetraoside is a fluorescent substrate that is used to measure the activity of the enzyme beta-glucuronidase. It is a chemiluminescent compound that emits light when hydrolyzed by an enzyme. 6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-cellotetraoside has been shown to be a high quality and environmentally friendly product, as it does not cause any significant change in pH or turbidity in water samples. It has also been shown to be nontoxic and nonmutagenic at concentrations up to 400 ppm. This product can be used for a variety of purposes, including diagnostic tests for bacterial infections or food testing.

Bacillus cereus is a rod-shaped β hemolytic bacterium commonly found in food and soil. Some strains can be associated with food intoxications, mainly from raw meat and poultry products. One example is the so called “fried rice syndrome”, that occurs when dishes containing cooked rice are left for several hours at room temperature. Typical symptoms of the infection include severe nausea, vomiting and diarrhea. Some strains of Bacillus cereus on the contrary are not harmful at all and they are used as probiotics food additives in animal farming to reduce the presence of other pathogens like Salmonella in the cecum end intestine. Bacillus cereus expresses phosphatidyl-choline specific phospholipase C (PC-PLC), encoded by the plc gene, which is regulated by PlcR. In addition, the expression of PlcR is dependent on the growth medium. Luc-B.cereus Na (L-8275) is a synthetic chemiluminescent substrate for the phosphatidyl-choline specific phospholipase C. The enzyme cleaves off phosphocholine from L-8275, leading to the release of D-Luc, and consequent strong light emission B. cereus culture exhibited strong luminescence, while RLU values were much lower for samples with B. thuringiensis.

4-Methylumbelliferyl 2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)-b-D-galactopyranoside is a fluorescent substrate for β–galactosidase. It is used to detect the enzyme in biological samples and as a diagnostic agent. This substrate can be used to develop chemiluminescent, chromogenic, and fluorogenic substrates for use in diagnostics. 4MUF 2OBG can also be conjugated to various ligands for use in bioluminescence assays or as a marker in immunoassays.

Aldol® 518 myo-inositol-1-phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-inositol specific phospholipase C activity (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC or PI-PLC-positive microorganisms grown in liquid or solid media, yielding an intense red coloration. In combination with Aldol® 355 fluorescence enhancer, red fluorescence can also obtained. Aldol® 518 myo-inositol-1-phosphate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

Aldol acetate is a chromogenic substrate that is used in food testing and diagnostics. Aldol acetate can be used in the detection of aminopeptidase and dipeptidyl peptidase activities, as well as in the determination of urinary proteins. Aldol acetate can also be used to detect anaerobic bacteria. This compound is conjugated with various ligands for use in immunoassays, such as beta-galactosidase, beta-glucuronidase, alpha-naphthyl phosphate ester, and phenyl phosphate ester. This product has been shown to be useful for chemiluminescent assays of DNA polymerases and reverse transcriptases in environmental samples. It has also been shown to be useful for bioluminescent assays of DNA polymerases and reverse transcriptases in environmental samples.

Bz-Gly-D-Lys-OH is a chemiluminescent substrate used for the detection of peroxidase, catalase, and other oxidoreductases. It is a high purity, high quality product that can be used in a variety of applications. This product has been shown to have an activity of approximately 10% at pH 4.0 and 100% at pH 8.0. Bz-Gly-D-Lys-OH has been tested for its ability to produce chemiluminescence in the presence of hydrogen peroxide and luminol. This product is still undergoing environmental testing and should not be used as a food additive or dietary supplement without further authorization from the FDA.

N-Benzoyl-Val-Gly-Arg p-nitroanilide hydrochloride is a fluorogenic substrate that can be used for the detection of β-glucuronidase. It has been shown to have high purity and quality, which makes it an excellent choice for use in food testing and staining.

Chromogenic substrate targeting amylase

Maltotetraose 2-aminobenzamide is a chemiluminescent substrate that emits light in the presence of certain oxidizing agents. It provides high quality and environmental testing, as well as staining and ligand capability. Maltotetraose 2-aminobenzamide is used in diagnostics to identify bacteria and other microorganisms. Maltotetraose 2-aminobenzamide can be used for the detection of bacterial colonies on agar plates using a fluorogenic substrate, or for the detection of fungi using a chromogenic substrate. Maltotetraose 2-aminobenzamide is also used as an enzyme substrate in food testing or conjugate with enzymes for bioluminescence.

4-Methylumbelliferyl 3,4,6-tri-O-acetyl-2-deoxy-2-trifluoroacetamido-a-D-glucopyranoside (4MUAA) is a fluorescent dye that is used for the detection of envelopment in cells. This dye binds to cell membranes and can be detected with a fluorescence microscope. 4MUAA can be used to determine the efficiency of an experimental methodology or algorithm by measuring the change in fluorescence intensity as a result of experimental treatment. The use of 4MUAA has been shown to be effective in determining whether or not there is an association between nationwide environmental pollution and cancer rates in Japan.

Aldol® 470 N-tosyl-L-alanine ester is a fluorogenic substrate that is used in enzyme and chromogenic assays. It is a high purity, high quality, and highly soluble substrate for chemiluminescent reactions. Aldol® 470 N-tosyl-L-alanine ester can be used to detect the presence of bacteria in culture media and as an indicator of bacterial growth. It is also used to test for the presence of different types of enzymes that catalyze the oxidation of alcohols.

5-Bromo-3-indolyl a-D-galactopyranoside is a fluorogenic substrate that can be used for the detection of beta-galactosidase activity. It is also used as an enzyme substrate in diagnostics and research, as well as a chromogenic substrate in staining. 5-Bromo-3-indolyl a-D-galactopyranoside has been shown to be an excellent conjugate for proteins, nucleic acids, and other biomolecules. This product can also be used in culture media for the cultivation of bacteria and yeast.

D-Alanine 7-amido-4-methylcoumarin free base is a chromogenic substrate that is used in diagnostic testing. It is used to identify the presence of beta-lactamase, which catalyzes the hydrolysis of penicillin and cephalosporin antibiotics, by turning the substrate into a bright blue color. D-Alanine 7-amido-4-methylcoumarin free base can also be used as a fluorescent substrate for bioluminescence or chemiluminescence reactions. This product is high quality and has CAS number 201847-51-0.

Aldol® 450 acetate is an enzyme substrate that is used in the detection of aldolase activity. Aldol® 450 acetate has been shown to be a useful alternative to the traditional bioluminescence and chemiluminescence methods for detecting aldolase activity. It has also been shown to be effective as a fluorogenic substrate for detecting aldolase activity in food products. Aldol® 450 acetate can be conjugated with other compounds, such as ligands and dyes, to enable it to detect different enzymes.
Aldol® 450 acetate has an absorption maximum at 450 nm and fluoresces blue-green under longwave UV light excitation. It is soluble in water and exhibits excellent stability at high temperatures (up to 50°C) and pH (1-14).

Resorufin benzyl ether is a chemical compound that can be used as a substrate for cytochrome P450. It has been shown to inhibit the activity of epoxide hydrolase, an enzyme involved in the metabolism of endoperoxides. Resorufin benzyl ether has also been found to inhibit cytochrome P450 enzymes, which are responsible for oxidative metabolism of xenobiotics. This compound has been shown to have a high affinity for mouse monoclonal antibody and is used in immunoassays to detect mouse antibodies. Resorufin benzyl ether is used in biochemical assays and polymerase chain reactions (PCRs) as a fluorescence marker. In addition, this compound is used in model systems to study enzyme activities such as recombinant cytochrome p450 and protein oxidation by reactive oxygen species (ROS).

4-Methylumbelliferyl-b-D-xylotrioside is a fluorogenic substrate that reacts with an enzyme in the presence of oxygen to produce light. It is used in diagnostic applications, such as detecting the presence of a specific enzyme or protein. 4-Methylumbelliferyl-b-D-xylotrioside can also be used as a chromogenic substrate for the detection of DNA and RNA. This compound can be conjugated with other molecules for use in immunoassays and other diagnostic applications. 4-Methylumbelliferyl-b-D-xylotrioside has been shown to bind to bacterial cells and activate them, which leads to the production of light through bioluminescence.

N-(4-Nitrophenyl)-D-glucopyranosylamine is a conjugate that is used in food testing, environmental testing, and diagnostics. It is a fluorogenic substrate that reacts with the enzyme glucose oxidase to produce a yellow color. This product has high purity and quality and CAS No. 553-03-6. It also has bioluminescence properties.

Colchicine fluorescein is the fluorogenic form of colchicine. It is used for staining assembly of tubulin. Colchicine-site inhibitors (abbrevated as CBSIs) disrupt tubulin polymerization. Colchicine fluorescein serves as a probe to study the binding of colchicine to tubulin, as the colchicine binding site is a promising target for discovering novel antitumour agents.

Aldol® 467 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 467 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 467 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

Caspase substrates are a class of pharmacological agents that regulate apoptosis. Caspases are proteases that cleave specific proteins in the cell, leading to apoptosis. Pro-apoptotic proteins activate caspases, while anti-apoptotic proteins inhibit them. Caspase substrates can be either pro-caspase or anti-caspase, depending on the desired effect. They may also be used to regulate caspases without affecting their activity. The caspase substrate is cleaved by caspases and releases a polypeptide fragment that triggers apoptosis in some cells, but not others. For example, the Hl-60 cell line is resistant to the pro-apoptotic effects of the substrate because it contains an anti-apoptotic protein called Bcl-2. In contrast, myocardial cells contain pro-apoptotic proteins and are sensitive to the pro-apoptotic effects of the substrate. In