Npys allows site-directed disulfide bond formation, as it is cleaved by thiols. Galande and Spatola obtained disulfide heterodimers of peptides via coupling Boc-Cys(NPys)-OH together with Fmoc-Cys(Mmt)-OH to MBHA-resin followed by Mmt removal with 1% TFA in dichloromethane. Mourier et al. used BCNP for the identification of T-cell epitopes in Cys and/or Cyt-containing proteins.
Technical inquiry about: 01-4014705 Boc-Cys(NPys)-OH
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