5-Bromo-4-chloro-3-indoxyl-beta-D-xylopyranoside

CAS: 207606-55-1

Ref. 3D-B-7750

Product Information

• Name: 5-Bromo-4-chloro-3-indoxyl-beta-D-xylopyranoside
• Synonyms: X-beta-D-xyloside
• CAS: 207606-55-1

Description

5-Bromo-4-chloro-3-indoxyl-beta-D-xylopyranoside is a fluorogenic substrate that can be used to measure the activity of bacterial esterases. The enzyme cleaves the xylose moiety from 5-bromo-4-chloro-3-indoxyl, yielding indoxyl as an intermediate product. This reaction can be catalyzed by esterases found in bacteria, which hydrolyze esters containing a single hydroxyl group at one end and a carboxylic acid or alcohol at the other end. Indoxyl is fluorescent and can be detected using a fluorimeter. 5BX is also chromogenic, so it can be used as an enzyme substrate for detecting beta galactosidase activity in bacteria such as Mycobacterium smegmatis or Escherichia coli.
5BX is also used to detect chemilumines

• Brand: Biosynth

Chemical properties

• Molecular weight: 378.61 g/mol
• Formula: C₁₃H₁₃BrClNO₅