![Cathepsin G FRET substrate [5-FAM]/[6-TAMRA]](https://static.cymitquimica.com/products/3D/img/CRB1100420.png "Click to enlarge")
Cathepsin G FRET substrate [5-FAM]/[6-TAMRA]
Ref. 3D-CRB1100420
| 1mg | 522.00 € | ||
| 100µg | 354.00 € | ||
| 500µg | 371.00 € |
Product Information
- 5fam-EPFWEDQ(K/6-Tamra)-NH2
Substrate peptide for Cathepsin G, a serine protease belonging to the chymotrypsin superfamily which acts as a physiologic regulator of platelet activation and thrombus formation. Cathepsin G also has antimicrobial activity and is involved in chemotaxis, apoptosis, the immune response and inflammation and hydrolysis of extracellular matrix proteins. Cathepsin G can cleave protease activated receptor-4 (PAR4) and is a potential target for novel anti-thrombotic therapies.This peptide contains the FRET pair: 5-Carboxyfluorescein (5-FAM) and 6-Carboxytetramethylrhodamine (6-TAMRA). 5-Carboxyfluorescein (5-FAM), which is present at the N-terminus of this peptide, is a widely used green fluorescent tag which excites at 495 nm and emits at 517 nm. 6-carboxytetramethylrhodamine (6-TAMRA), which is attached at the C-terminus, is a widely used fluorescent dye which excites at 546 nm and emits at 579 nm. In the intact peptide 6-TAMRA acts as a quencher of the fluorescence of 5-FAM therefore no fluorescence can be detected. However hydrolysis of the peptide between the donor/acceptor pair allows the fluorescence of 5-FAM to be recovered, enabling the quantitative measurement of enzymatic activity.Fluorescence Resonance Energy Transfer (FRET) peptides are convenient tools for the study of peptidase specificity and enzymatic activity.
Chemical properties
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