Acetyl-Histone H4 (1-21) K5Ac, K8Ac, K12Ac, K16Ac-GG-[Lys(5-FAM)]

Ref. 3D-CRB1101268

Product Information

• Name: Acetyl-Histone H4 (1-21) K5Ac, K8Ac, K12Ac, K16Ac-GG-[Lys(5-FAM)]
• Synonyms: Ac-SGRG(K/Ac)GG(K/Ac)GLG(K/Ac)GGA(K/Ac)RHRKVGG(K/5fam)-NH2Ac-SGRG-[K(Ac )]-GG-[K(Ac)]-GLG-[K(Ac)]-GGA-[K(Ac)]-RHRKV-GG-[K(5-FAM)]-am ideAc-Ala-Cys-Ser-Gly-Arg-Gly-[Lys(Ac)]-Gly-Gly-[Lys(Ac)]- Gly-Leu-Gly-[Lys(Ac)]-Gly-Gly-Ala-[Lys(Ac)]-Arg-His-Arg-Lys-V

Description

Histone 4 (H4) is one of the four core histones (H2A, H2B, H3 and H4) which are essential for compacting eukaryotic DNA into the nucleosome. Due to the high lysine and arginine content, histones have a net positive charge and therefore electrostatically interact with negatively charged DNA. The nucleosome arises when 147 base pairs of DNA wrap around a H3-H4 tetramer and two H2A-H2B dimers, forming the histone octamer core. Like other core histones, H4 has a globular domain and a flexible N-terminal domain, the histone tail, which can undergo modifications such as acetylation, methylation, phosphorylation and ubiquitination.Gene transcriptional activation or inactivation is controlled by ATP-dependent chromatin remodelling factors and histone modifying enzymes. Both processes function to alter the positioning of the nucleosome, allowing the DNA within to be either accessible to the transcription machinery or inaccessible. H4 lysine rich tail plays a role in the higher order chromatin folding.The lysines at positions 5, 8, 12 and 16 have been acetylated, which neutralizes the positive charge on the amino acid, loosening the chromatin structure. This alteration to the accessibility of chromatin promotes the initiation of transcription. Acetyl-Histone H4 (1-21) K5Ac, K8Ac, K12Ac, K16Ac-GG-[Lys(5-FAM)]-has a C-terminal GGK linker labelled with 5-Carboxyfluorescein (5-FAM), a widely used green fluorescent tag. Additionally, this peptide has an uncharged C-terminal amide and is protected from N-terminal modifications by a covalently bonded acetyl group.

• Brand: Biosynth

Chemical properties

• Molecular weight: 2,899.5 g/mol