6-Chloro-3-indolyl b-D-galactopyranoside

CAS: 138182-21-5

Ref. 3D-EC05439

Product Information

• Name: 6-Chloro-3-indolyl b-D-galactopyranoside
• Synonyms: Salmon Gal6-Chloro-3-(b-D-galactopyranosyloxy)indoleRose GalRed-Gal, 6-Chloro-1H-indol-3-yl β-<span class="text-smallcaps">D</span>-galactopyranoside, 6-Chloro-3-indolyl--D-galactopyranoside, 6-chloro-1H-indol-1-yl beta-D-galactopyranoside, 6-chloro-1H-indol-3-yl β-D-galactopyranoside, ChloroindolylDgalactopyranoside, Red-Gal(r) 6-Chloro-3-indolyl-!-D-galactoside, Salmon Galactoside, β-<span class="text-smallcaps">D</span>-Galactopyranoside, 6-chloro-1H-indol-3-yl

Description

6-Chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase that yields a salmon or rose-coloured precipitate and hence is also known as Salmon-Gal. It can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside (IPTG) as an alternative to the chromogenic substrate 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-Gal) for detection of beta-galactosidase activity in bacterial colonies in colorimetric assays. Where beta-galactosidase expressed from a reporter gene in molecular biology experiments, recombinant colonies on growth plates are salmon-coloured while non-recombinants appear as white. Use of nitroblue tetrazolium salt (NBT) with Salmon-Gal may provide faster results, yielding a clear, purple precipitate, than for Salmon-Gal alone. In conjunction with 5-bromo-4-chloro-3-indolyl-beta-D-glucoside (X-Glc), Salmon-Gal is useful for simultaneous detection of GUS and Lac activities in the same medium.

• Brand: Biosynth

Chemical properties

• Molecular weight: 329.73 g/mol
• Formula: C₁₄H₁₆ClNO₆
• Purity: Min. 98 Area-%
• Color/Form: Powder
• InChI: InChI=1S/C14H16ClNO6/c15-6-1-2-7-8(3-6)16-4-9(7)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
• InChI key: InChIKey=OQWBAXBVBGNSPW-MBJXGIAVSA-N
• SMILES: OC[C@H]1O[C@@H](Oc2c[nH]c3cc(Cl)ccc23)[C@H](O)[C@@H](O)[C@H]1O