6,8-Difluoro-4-methylumbelliferyl phosphate

CAS: 214491-43-7

Ref. 3D-FD87907

Product Information

• Name: 6,8-Difluoro-4-methylumbelliferyl phosphate
• Synonyms: DiFMUP, 2H-1-Benzopyran-2-one, 6,8-difluoro-4-methyl-7-(phosphonooxy)-, 6,8-Difluoro-4-methyl-7-(phosphonooxy)-2H-1-benzopyran-2-one

Description

6,8-Difluoro-4-methylumbelliferyl phosphate is a fluorescent probe for the detection of phosphatases. The compound is converted to an active form by the enzyme, and reacts with the substrate p-nitrophenyl phosphate in vitro. This reaction will produce a change in fluorescence intensity and can be detected using a spectrophotometer. 6,8-Difluoro-4-methylumbelliferyl phosphate has been used to study protein phosphorylation in vitro by inhibiting the enzymatic activity of protein phosphatase 2A (PP2A). The microcapsule assay is a rapid, sensitive method for detecting phosphatases that react with 6,8-difluoro-4-methylumbelliferyl phosphate.

• Brand: Biosynth

Chemical properties

• Molecular weight: 292.13 g/mol
• Formula: C₁₀H₇F₂O₆P
• Purity: Min. 95%
• InChI: InChI=1S/C10H7F2O6P/c1-4-2-7(13)17-9-5(4)3-6(11)10(8(9)12)18-19(14,15)16/h2-3H,1H3,(H2,14,15,16)
• InChI key: InChIKey=DZANYXOTJVLAEE-UHFFFAOYSA-N
• SMILES: Cc1cc(=O)oc2c(F)c(OP(=O)(O)O)c(F)cc12