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Glycine-2,2-D2 is a nutrient that can be found in the human body. It is synthesized by the deamination of glutamic acid, an endogenous amino acid. Glycine-2,2-D2 is used as a tracer for human urine and has been detected in the mass spectrometry of human urine samples with acetonitrile. It can also be used to calibrate methods such as chromatography or analytical methods for validation.