Naphthol AS-BI-phosphate

CAS: 1919-91-1

Ref. 3D-FN15225

Product Information

• Name: Naphthol AS-BI-phosphate
• Synonyms: 2-Naphth-o-anisidide, 7-bromo-3-hydroxy-, dihydrogen phosphate, 2-Naphth-o-anisidide, 7-bromo-3-hydroxy-, dihydrogen phosphate (ester), 2-Naphth-o-anisidide, 7-bromo-3-hydroxy-, phosphate, 2-Naphthalenecarboxamide, 7-Bromo-N-(2-Methoxyphenyl)-3-(Phosphonooxy), 6-Bromo-2-Phosphohydroxy-3-Naphthoic Acid O-Anisidide, 6-Bromo-3-[(2-Methoxyphenyl)Carbamoyl]Naphthalen-2-Yl Dihydrogen Phosphate, 7-Bromo-3-Hydroxy-2-Naphthoic-O-Aniside Phosphate, 7-Bromo-3-Hydroxy-2-Naphthoic-O-Anisidide Phosphate, 7-Bromo-N-(2-Methoxyphenyl)-3-(Phosphonooxy)-2-Naphthalenecarboxamide, Nabp, Naphthol As-Bi Phosphoric Acid, Naphthol As-Bl Phosphoric Acid
• CAS: 1919-91-1

Description

Naphthol AS-BI-phosphate is a substrate for the enzyme acid phosphatase. It is used as a histochemical stain in human serum, which can be applied to detect the presence of alkaline phosphatase in tissue. Naphthol AS-BI-phosphate also has protease activity and inhibits the enzyme activity of diazonium salt. It is a substrate for p-nitrophenyl phosphate and undergoes oxidation, forming an acid product that can be detected by acid phosphatase. This product reacts with nitroblue tetrazolium (NBT) to produce a blue precipitate, which can be observed with a microscope or under UV light. The optimum pH for this reaction is 7.5 and it is inhibited by disulfide bonds.

• Brand: Biosynth

Chemical properties

• Molecular weight: 452.19 g/mol
• Formula: C₁₈H₁₅BrNO₆P
• Color/Form: Powder
• InChI: InChI=1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24)
• InChI key: InChIKey=HUXIAXQSTATULQ-UHFFFAOYSA-N
• SMILES: COc1ccccc1NC(=O)c1cc2cc(Br)ccc2cc1OP(=O)(O)O