Product Information
Dioxetane 12 is a hydroxy-containing compound that reacts with metal ions to produce chemiluminescence. Dioxetane 12 can be detected by chemiluminescent techniques, such as the use of a luminol-based reagent. The reaction mechanism of dioxetane 12 is similar to that of luminol in that it involves oxidation of a primary amine to an imine and subsequent release of hydrogen peroxide. The presence of a fatty acid moiety on the dioxetane 12 molecule enhances its reactivity with metal ions and increases the intensity of chemiluminescence. This chemiluminescent detection property makes dioxetane 12 useful for immunoassays, such as those for measuring levels of fatty acids or imines in biological samples. Dioxetane 12 has been shown to be useful for detecting hybridization between DNA strands.
