Human CGa(Chorionic Gonadotropin α Polypeptide) ELISA Kit
Ref. EK-ELK5082
48T | Discontinued | ||
96T | Discontinued |
Product Information
- FSHA; GPHA1; GPHa; HCG; LHA; TSHA; Thyroid Stimulating Hormone Alpha; Follicle-Stimulating Hormone Alpha Subunit; Luteinizing hormone alpha chain; Lutropin alpha chain
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CGa protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CGa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CGa in the samples is then determined by comparing the OD of the samples to the standard curve.