N-(5-Amino-1-carboxypentyl)iminodiacetic Acid Tri-t-butyl Ester

CAS: 205379-08-4

Ref. TR-A603260

Product Information

• Name: N-(5-Amino-1-carboxypentyl)iminodiacetic Acid Tri-t-butyl Ester
• Synonyms: 6-amino-2-[bis[2-[(2-methylpropan-2-yl)oxy]-2-oxoethyl]amino]hexanoic acid

Description

Stability Moisture Sensitive
Applications A nitrilotriacetic acid derivative used as a metal chelating adsorbent for metal ion affinity chromatography. This method can be used for identification and rapid one-step purification of gene products expressed as fusion proteins with an oligo-histidine tag. The oligo-histidine tag serves as a high affinity binding sequence for the purification of fusion proteins via a metal chelating absorbent such as N-(5-Amino-1-carboxypentyl)iminodiacetic acid.Also soluble in DMSO:DMF 1:1 and DMSO:CH2Cl2 1:2
References Hochuli, E., et al.: J. of Chromatography, 411, 177 (1987)

• Brand: TRC

Chemical properties

• Molecular weight: 374.47
• Formula: C₁₈H₃₄N₂O₆
• Color/Form: Neat
• InChI: InChI=1S/C18H34N2O6/c1-17(2,3)25-14(21)11-20(12-15(22)26-18(4,5)6)13(16(23)24)9-7-8-10-19/h13H,7-12,19H2,1-6H3,(H,23,24)
• InChI key: InChIKey=SLEOTGKREMTOTD-INIZCTEOSA-N
• SMILES: CC(C)(C)OC(=O)CN(CC(=O)OC(C)(C)C)[C@@H](CCCCN)C(=O)OC(C)(C)C