7-Azido-4-methylcoumarin

CAS: 95633-27-5

Ref. TR-A884803

Product Information

• Name: 7-Azido-4-methylcoumarin
• Synonyms: 7-azido-4-methylchromen-2-one, 7-Azido-4-Methylcoumarin

Description

Applications 7-Azido-4-methylcoumarin is a highly sensitive and selective fluorogenic H2S probe. 7-Azido-4-methylcoumarin is a tool for monitoring the activity of pyridoxal-5'-phosphate (PLP)-dependent enzymes (e.g. cystathionine β-synthase (CBS), cystathionine γ-lyase (CGL) and tryptophan synthase (TS)) and to identify novel cystathionine β-synthase (CBS) inhibitors and activators. The aromatic azide moiety is selectively reduced in the presence of H2S, producing the fluorescent 7-amino-4-methylcoumarin (AMC) with a concomitant increase in fluorescence with ?ex = 365 nm and ?em = 450 nm. It has been characterized as a novel fluorescent photoactive probe for use in photoaffinity labeling of the substrate binding site of human phenol sulfotransferase (SULT1A1 or P-PST-1)
References Chen G, et al. Protein Sci. 1999 Oct;8(10):2151-7.Collom SL el al. Arch Biochem Biophys. 2007 Mar 1;459(1):59-69

• Brand: TRC

Chemical properties

• Molecular weight: 201.181
• Formula: C₁₀H₇N₃O₂
• Color/Form: Neat
• InChI: InChI=1S/C10H7N3O2/c1-6-4-10(14)15-9-5-7(12-13-11)2-3-8(6)9/h2-5H,1H3
• SMILES: Cc1cc(=O)oc2cc(N=[N+]=[N-])ccc12