Fluorescein Bis(methylene)]bis[N-(carboxymethyl)glycine] Sodium Salt (Technical Grade)

CAS: 207124-64-9

Ref. TR-F485290

Product Information

• Name: Fluorescein Bis(methylene)]bis[N-(carboxymethyl)glycine] Sodium Salt (Technical Grade)
• Synonyms: N,N'-[(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-4',5'-diyl)bis(methylene)]bis[N-(carboxymethyl)glycine Sodium Salt
• CAS: 207124-64-9

Description

Applications Fluorescein Bis(methylene)]bis[N-(carboxymethyl)glycine] is an intermediate used in the synthesis of Calcein AM (C125400), which is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein (sc-202090), which is an extremely fluorescent and negatively charged molecule. Calcein-AM can be used to stain living cells and in cytoplasmic Schwann cells has been observed to fluoresce bright green. Calcein-AM has been reported to be a neutral substrate for P-glycoprotein (Pgp) and multidrug resistance protein (MRP) and has been used in flow cytometry studies to analyze the function of P-gp and MRP.
References Feller, N., et al.: Br. J. Cancer, 72, 543 (1995); Dhar, S., et al.: Eur. J. Pharmacol., 346, 315 (1998); Essodaigui, M., et al.: Biochemistry, 37, 2243 (1998); Lemasters, J.J., et al.: Biochim. Biophys. Acta., 1366,177 (1998);

• Brand: TRC

Chemical properties

• Molecular weight: 622.53 + x(22.99)
• Formula: C₃₀H₂₆N₂O₁₃•xNa
• Color/Form: Neat
• InChI: InChI=1S/C30H26N2O13.Na/c33-21-7-5-19-27(16(21)9-31(11-23(35)36)12-24(37)38)44-28-17(10-32(13-25(39)40)14-26(41)42)22(34)8-6-20(28)30(19)18-4-2-1-3-15(18)29(43)45-30;/h1-8,33-34H,9-14H2,(H,35,36)(H,37,38)(H,39,40)(H,41,42)
• InChI key: InChIKey=KPUIOAAOQUWUNH-UHFFFAOYSA-N
• SMILES: O=C(O)CN(CC(=O)O)Cc1c(O)ccc2c1Oc1c(ccc(O)c1CN(CC(=O)O)CC(=O)O)C21OC(=O)c2ccccc21