CAS 76823-03-5

5-carboxifluoresceína

Descrição:

5-carboxifluoresceína é um corante fluorescente comumente usado em várias aplicações bioquímicas e analíticas, particularmente em microscopia de fluorescência e citometria de fluxo. É caracterizado por sua fluorescência verde brilhante, que é atribuída à sua estrutura de xanteno. O composto apresenta um grupo ácido carboxílico que melhora sua solubilidade em soluções aquosas, tornando-o adequado para estudos biológicos. Sua estrutura molecular permite uma ligação eficaz a proteínas e ácidos nucleicos, facilitando seu uso como traçador ou marcador em imagens celulares. Além disso, 5-carboxifluoresceína é frequentemente empregado na síntese de sondas fluorescentes e no desenvolvimento de ensaios para detectar atividade enzimática. O corante exibe estabilidade sob uma variedade de condições de pH, embora sua fluorescência possa ser afetada por fatores ambientais, como pH e força iônica. No geral, 5-carboxifluoresceína é uma ferramenta versátil no campo da bioquímica e biologia molecular, permitindo que os pesquisadores visualizem e quantifiquem processos biológicos com alta sensibilidade.

Fórmula: C₂₁H₁₂O₇

InChI: InChI=1/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

Chave InChI: InChIKey=NJYVEMPWNAYQQN-UHFFFAOYSA-N

SMILES: O=C1OC2(C=3C1=CC(C(O)=O)=CC3)C=4C(OC=5C2=CC=C(O)C5)=CC(O)=CC4

Sinónimos:

• 3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
• 3′,6′-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylic acid
• 3′,6′-Dihydroxy-3-oxospiro[2-benzofuran-1,9′-xanthene]-5-carboxylic acid
• 3′,6′-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-5-carboxylic acid
• 5-Fam
• Carboxyfluorescein,5-
• FAM (dye)
• Fam 494/520
• Flamma 456 Carboxylic acid
• Hydroxyfluorescein
• Spiro[isobenzofuran-1(3H),9′-[9H]xanthene]-5-carboxylic acid, 3′,6′-dihydroxy-3-oxo-
• 5-Carboxyfluorescein
• FAM
• 3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
• 5-CARBOXYLUORESCEIN
• 5(6)-FAM
• 5-Carboxyfluorescein (5-FAM)
• 4-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-ISOPHTHALIC ACID
• 3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5(6)-carboxylic acid
• 4-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-isophth
• 5-(AND-6)-CARBOXY SNAFL(R)-1
• 4(5)-CARBOXYFLUORESCEIN
• 5-(AND-6)-CARBOXYFLUORESCEIN
• 5-(AND-6)-CARBOXY SNARF(R)-1
• 4-carboxyfluorescein
• 5-(AND-6)-CARBOXY SNAFL(R)-2
• 5-CF

5-Carboxyfluorescein

CAS: 76823-03-5

Fórmula: C₂₁H₁₂O₇

Pureza: >97.0%(T)(HPLC)

Cor e Forma: Light yellow to Brown powder to crystal

Peso molecular: 376.32

Ref: 3B-C2477

5-Carboxyfluorescein, single isomer, 97%

CAS: 76823-03-5

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Sci

Fórmula: C₂₁H₁₂O₇

Pureza: 97%

Cor e Forma: Powder or crystals or crystalline powder, Yellow to orange

Peso molecular: 376.32

Ref: 02-H66949

5-FAM

CAS: 76823-03-5

5-FAM, a carboxylic acid, reacts with amines for biomolecule labeling; excites at 488 nm, high fluorescence, solubility, absorbance.

Fórmula: C₂₁H₁₂O₇

Pureza: 98.28% - 99.32%

Cor e Forma: Yellow Solid

Peso molecular: 376.32

Ref: TM-T14050

5-FAM

CAS: 76823-03-5

5-FAM

Fórmula: C₂₁H₁₂O₇

Pureza: ≥98%

Peso molecular: 376.32

Ref: 54-BUP14627

5-Carboxyfluorescein

CAS: 76823-03-5

Fórmula: C₂₁H₁₂O₇

Pureza: ≥ 99.0%

Cor e Forma: Yellow to orange powder

Peso molecular: 376.3

Ref: 7W-GT3783

5-Carboxyfluorescein

CAS: 76823-03-5

5-Carboxyfluorescein

Fórmula: C₂₁H₁₂O₇

Pureza: 98%

Cor e Forma: Solid

Peso molecular: 376.31577

Ref: 54-BIC1050

5-Carboxyfluorescein (Technical Grade)

Produto Controlado

CAS: 76823-03-5

Applications 5-Carboxyfluorescein is a useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent. Dyes and metabolites.
References Theisen, P., et al.: Tetrahedron Lett., 33, 5033 (1992); Mattingly, P.: Bioconjgate Chem., 3, 430 (1992); Bertrand, R., et al.: Biochemistry, 34, 9500 (1995);

Fórmula: C₂₁H₁₂O₇

Cor e Forma: Neat

Peso molecular: 376.32

Ref: TR-C178320

5-Carboxyfluorescein

CAS: 76823-03-5

5-Carboxyfluorescein (5-FAM) is a self-quenching dye and one of the most common labelling agents for biomolecules, cell staining and localisation studies. The fluorescence is pH dependent, therefore it has been used to measure the internal pH of cells. As it is only membrane permeable to dead cells, it can be used as a staining agent to distinguish viable and non-viable cells. It is also used in PCR to label the 5’ end of oligonucleotides in the presence of a quencher at the 3’ end (dual labelled probe). During the amplification process, the dye is cleaved and the fluorescence increases proportionally with the amount of the specific sequence amplified during the PCR process. The development of the fluorescence signal is therefore specifically related to the amplification of the target sequence. The carboxylic acid can be activated to react with a primary amine.

Fórmula: C₂₁H₁₂O₇

Pureza: Min. 98 Area-%

Cor e Forma: Yellow Powder

Peso molecular: 376.32 g/mol

Ref: 3D-FC19773

3′,6′-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylic acid

CAS: 76823-03-5

Fórmula: C₂₁H₁₂O₇

Pureza: 95%

Cor e Forma: Solid

Peso molecular: 376.32

Ref: 10-F448180

5-FAM

CAS: 76823-03-5

3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid

Fórmula: C₂₁H₁₂O₇

Pureza: 97%

Peso molecular: 376.32

Ref: IN-DG243928

5-Carboxyfluorescein

CAS: 76823-03-5

Fórmula: C₂₁H₁₂O₇

Peso molecular: 376.32

Ref: ST-EA-CP-F116029

5-Carboxy-fluorescein

CAS: 76823-03-5

Widely used green-fluorescent labeling dye.

Fórmula: C₂₁H₁₂O₇

Pureza: > 98%

Cor e Forma: Yellow Powder

Peso molecular: 376.32

Ref: 01-4045141

3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid

CAS: 76823-03-5

Fórmula: C₂₁H₁₂O₇

Pureza: 97%

Cor e Forma: Solid

Peso molecular: 376.3158

Ref: IN-DA008P3R