Outros

5-Bromo-3-indolyl phosphate disodium salt is a chemiluminescent substrate that is used for the detection of bacterial activity. It reacts with oxygen to produce light, which can be measured using a luminometer. It is also used as a chromogenic substrate for detection of bacterial activity in culture media. The enzyme reaction produces an intense blue fluorescence and is used for diagnostic purposes in food testing and medical diagnostics. 5-Bromo-3-indolyl phosphate disodium salt has been shown to be effective against many bacteria and fungi, including Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger. This product is chemically stable at room temperature but should be stored at 4°C or below.

Chromogenic substrate for esterase with C9 activity yielding a salmon colored precipitate upon cleavage.

2-Nitrophenyl butyrate is a chromogenic enzyme substrate that is commonly used in enzymatic assays to detect lipases and esterases. When hydrolyzed by these enzymes, the substrate yields a yellow product that can be detected spectrophotometrically at 405 nm. The advantage of using 2-nitrophenyl butyrate is its high sensitivity and specificity for detecting lipolytic and esterolytic activities. This substrate is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the determination of esterase activity in biological samples such as serum, plasma, and cell lysates.

6-Chloro-3-indolyl b-D-glucuronide sodium salt is a chromogenic substrate for beta-glucuronidase, a reporter gene used to monitor gene expression by detecting the enzyme's activity. It yields a salmon colored precipitate upon cleavage.

3,3'-Diaminobenzidine (DAB) is an organic compound derived from benzidine. DAB is water-soluble as the tetrahydrochloride salt and is used in immunohistochemical staining of nucleic acids and proteins. Within research in Alzheimer's disease, Aβ protein amyloid plaques are targeted by a primary antibody, and subsequently by a secondary antibody, which is conjugated with a peroxidase enzyme. This will bind DAB as a substrate and oxidize it, producing an easily observable brown colour. 3,3’-Diaminobenzidine tetrahydrochloride hydrate is also available as the tetrahydrochloride salt and as the free base.

4-Nitrophenyl alpha-D-glucuronide is a chromogenic substrate widely used for detecting and quantifying the activity of alpha glucuronidase enzyme. Upon cleavage by the enzyme, it releases the yellow-colored 4-nitrophenol, whose absorbance can be monitored spectrophotometrically. This substrate is routinely employed in biochemistry and molecular biology applications, such as enzyme assays, enzyme kinetics studies, and characterization of recombinant enzymes. As a non-toxic and easy-to-use reagent, 4-Nitrophenyl alpha-D-glucuronide provides a convenient and sensitive method for studying alpha-glucuronidase activity in various biological samples, including cell extracts, tissue homogenates, and purified enzyme preparations.

5-Amino-3-indoxyl-beta-D-galactopyranoside (AIG) is a chromogenic substrate that is used for the detection of beta-galactosidase activity. AIG is also used in food testing, diagnostics, and environmental testing. The detection of beta-galactosidase activity can be done by either using the fluorogenic substrate 4-methylumbelliferyl galactopyranoside or the bioluminescence system of firefly luciferase and luciferin. This product has a CAS number of 110114-03-0 and a molecular weight of 417.5 g/mol.

5-Bromo-6-chloro-3-indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in mucopolysaccharidosis screening.

4-Nitrophenyl-alpha-D-glucopyranoside, beta-Anomer is a chromogenic sugar that can be used as an indicator for the presence of 4-nitrophenol. It is a highly reactive compound that spontaneously reacts with nitrobenzene to form a dinitroaniline. This anomeric structure is easily oxidized by air and light to form the corresponding o-dinitroaniline. When this reaction occurs, this substance changes color from yellow to red.

Aldol® 484 alpha-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate for alpha-glucosidase activity (EC 3.2.1.20, alpha-1,4-glucosidase, also known as maltase). The colorless enzyme substrate is cleaved by bacteria producing this enzyme in liquid or solid media, yielding orange color. Green fluorescence is also generated if a suitable matrix is present such as: cellulose acetate filters, polypropylene tubes or microplates and polyethylene vials. Aldol® 484 alpha-D-glucopyranoside can be used under aerobic and anaerobic conditions.

Aldol 495 nonanoate is a bioluminescent substrate that can be used to measure the activity of Aldolase, which is an enzyme that catalyzes the conversion of glyceraldehyde-3-phosphate and dihydroxyacetone phosphate to D-glyceraldehyde. This substrate is used in various diagnostic assays, including food testing and staining. Aldol 495 nonanoate was developed by Cymit Quimica Pharmaceuticals and has been patented by EP 2427431 and US 8940909.