Substratos enzimáticos
Substratos enzimáticos são moléculas sobre as quais as enzimas atuam para catalisar reações químicas. O substrato liga-se ao sítio ativo da enzima, onde passa por uma transformação específica para formar o produto da reação. Os substratos enzimáticos são ferramentas essenciais na pesquisa para estudar a cinética enzimática, especificidade e mecanismo de ação. Eles também são usados em ensaios diagnósticos e estudos bioquímicos. Na CymitQuimica, oferecemos uma seleção diversificada de substratos enzimáticos de alta qualidade para apoiar suas pesquisas em enzimologia e bioquímica.
Subcategorias de "Substratos enzimáticos"
Foram encontrados 1529 produtos de "Substratos enzimáticos"
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6-Chloro-3-indoxyl-beta-D-galactopyranoside, plant origin
CAS:6-Chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase that yields a salmon or rose-coloured precipitate and hence is also known as Salmon-Gal. It can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside (IPTG) as an alternative to the chromogenic substrate 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-Gal) for detection of beta-galactosidase activity in bacterial colonies in colorimetric assays. Where beta-galactosidase expressed from a reporter gene in molecular biology experiments, recombinant colonies on growth plates are salmon-coloured while non-recombinants appear as white. Use of nitroblue tetrazolium salt (NBT) with Salmon-Gal may provide faster results, yielding a clear, purple precipitate, than for Salmon-Gal alone. In conjunction with 5-bromo-4-chloro-3-indolyl-beta-D-glucoside (X-Glc), Salmon-Gal is useful for simultaneous detection of GUS and Lac activities in the same medium.Fórmula:C14H16ClNO6Pureza:Min. 98.0 Area-%Peso molecular:329.74 g/molLipase substrate
CAS:Synthetic chromogenic substrate for detection of lipase enzyme activity that produces a red coloured product, Suitable for use in serum of plasma matrices. This lipase substrate is commonly used in veterinary diagnostics for monitoring and detecting canine pancreatitis,Fórmula:C45H69NO8Peso molecular:752.03 g/molAldol® 455 alpha-D-glucopyranoside - Biosynth Patent: EP 2427431 and US 8940909
Aldol® 455 alpha-D-glucopyranoside is a high quality, environmentally tested, staining, and ligand for bioluminescent assays. It has been shown to be a chromogenic substrate for diagnostics and a fluorogenic substrate for enzymes such as alpha-glucosidase, producing a yellow/green-orange color. Aldol® 455 alpha-D-glucopyranoside is also used in culture media and food testing.
Fórmula:C27H24ClNO7Pureza:Min. 95 Area-%Cor e Forma:PowderPeso molecular:509.93 g/mol2-Fluoro-4-nitrophenyl b-D-galactopyranoside
CAS:2-Fluoro-4-nitrophenyl b-D-galactopyranoside is a chromogenic substrate in enzymatic assays with β-galactosidase. Commonly abbreviated as PFONPG, it is a hydrophilic compound that can be dissolved in saline and blood samples, which makes it a suitable candidate for diagnostic applications as a gene reporter probe by simple analytic methodologies. 2-Fluoro-4-nitrophenyl b-D-galactopyranoside have been used to detect and monitor the growth of prostate tumor cells.Pureza:Min. 95%Cor e Forma:PowderPeso molecular:319.24 g/mol4-Methylumbelliferyl-2-O-(a-L-fucopyranosyl)-a-L-fucopyranoside
4-Methylumbelliferyl-2-O-(alpha-L-fucopyranosyl)-alpha-L-fucopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl-2-O-(alpha-L-fucopyranosyl)-alpha-L-fucopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for fucosidosis diagnosis.
Pureza:Min. 95%Cor e Forma:White PowderPeso molecular:468.45 g/mol4-Nitrophenyl-alpha-L-fucopyranoside
CAS:Chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of α-L-fucosidase, especially in human serum.Fórmula:C12H15NO7Pureza:Min. 98 Area-%Peso molecular:285.26 g/molEthyl umbelliferone-3-carboxylate-α-D-glucopyranoside
Ethyl umbelliferone-3-carboxylate-a-D-glucopyranoside is a fluorogenic substrate used for detecting alpha-glucosidase activity in enzymatic assays. When alpha-glucosidase cleaves , the glycosidic bond, umbelliferone-3-carboxylate is released, which exhibits strong fluorescence. This substrate is commonly utilized in glycobiology research, diagnostic tests, and high-throughput screening of enzyme inhibitors.
Fórmula:C18H20O10Pureza:Min. 95%Cor e Forma:Off-white to yellow solid.Peso molecular:396.35 g/mol4-Methylumbelliferyl 2-acetamido-2-deoxy-b-D-glucopyranoside-6-sulfate sodium salt
CAS:4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is a high-quality fluorogenic substrate specifically designed for the detection and quantification of various enzymes, including beta-glucosidases and sulfatases. Upon hydrolysis by the target enzyme, this substrate releases the highly fluorescent 4-methylumbelliferone, allowing for real-time monitoring and accurate measurement of enzyme activities in biochemical assays, diagnostic tests, and research applications. 4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt has excellent solubility in water, ensuring a consistent and reliable performance in different experimental conditions. This versatile substrate is an essential tool for researchers and industry professionals who require a sensitive and reproducible detection method for enzymes of interest.Fórmula:C18H20NO11SNaPureza:Min. 98 Area-%Cor e Forma:White PowderPeso molecular:481.41 g/mol5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, cyclohexylammonium salt
5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, cyclohexylammonium salt is a chemiluminescent substrate that provides a significant increase in light emission. It has a CAS number of 59820-92-0 and is supplied in high purity and high quality. This product is used for the detection of various biological reactions, including diagnostics, enzyme substrates, food testing, and more.Pureza:Min. 95%Resorufin β-D-cellobioside
CAS:Resorufin beta-D-cellobioside is a fluorogenic substrate for the enzyme beta-glucosidase. It is soluble in water and can be used as a chromogenic substrate for beta-galactosidase. Resorufin beta-D-cellobioside is also used as a ligand to study protein interactions, such as those between lectins and glycoproteins. This product is of high purity and quality, with CAS No. 1000404-48-7.Fórmula:C24H27NO13Pureza:Min. 95 Area-%Peso molecular:537.47 g/molRef: 3D-R-2056
10mgA consultar25mgA consultar50mgA consultar100mgA consultar250mgA consultar-Unit-ggA consultarL-Phenylalanine 7-amido-4-methylcoumarin trifluoroacetate salt
CAS:L-Phenylalanine 7-amido-4-methylcoumarin trifluoroacetate salt is a fluorogenic peptide substrate for aminopeptidase. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.Fórmula:C19H18N2O3•C2HF3O2Pureza:Min. 98 Area-%Cor e Forma:White PowderPeso molecular:436.38 g/mol4-Nitrophenyl 2-O-trans-feruloyl-a-L-arabinofuranoside
CAS:4-Nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside is a chromogenic substrate for alpha-L-arabinofuranosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside is used in different applications such as the Plant biomass analysis, bacterial arabinan degradation assays
Fórmula:C21H21NO10Pureza:Min. 95%Cor e Forma:PowderPeso molecular:447.39 g/molN-Acetyl-glycine p-nitroanilide
CAS:Substrate for hydrolasesFórmula:C10H10N2O5Pureza:Min. 95%Cor e Forma:Yellow powder.Peso molecular:238.2 g/mol2-Nitrophenyl b-D-glucopyranoside
CAS:Chromogenic substrate for beta-glucosidase
Fórmula:C12H15NO8Pureza:Min. 99 Area-%Cor e Forma:Off-White Yellow PowderPeso molecular:301.25 g/mol4-Methylumbelliferone
CAS:4-Methylumbelliferone is a coumarin derivative that inhibits the activity of enzymes such as p-nitrophenyl phosphate oxidase and aminopeptidase. It is used in vitro to study oxidative injury, which plays a role in the pathogenesis of inflammatory diseases, including primary sclerosing cholangitis and bone marrow transplantation. 4-Methylumbelliferone has been shown to induce apoptosis by increasing reactive oxygen species levels and activating toll-like receptor 4 (TLR4). It also has inhibitory effects on osteosarcoma cell proliferation in vitro.Fórmula:C10H8O3Peso molecular:176.17 g/mol4-Methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-β-D-glucopyranoside
4-Methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranoside is a fluorogenic substrate for beta-glucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranosideas a substrate for measuring the beta-glucosidase activity is used for Gaucher disease diagnosis.Fórmula:C24H26O11SPureza:Min. 95%Cor e Forma:White to off-white solid.Peso molecular:522.52 g/mol4-Methylumbelliferyl heptanoate
CAS:Lipase targeting MOSCERDAM® substrateFórmula:C17H20O4Pureza:Min. 95%Cor e Forma:White PowderPeso molecular:288.34 g/molIsopropyl-β-D-thiogalactopyranoside - non-animal origin
CAS:A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.Fórmula:C9H18O5SPureza:Min. 99.0 Area-%Peso molecular:238.3 g/mol3-Nitrophenyl b-D-galactopyranoside
CAS:Chromogenic substrate targeting beta-galactosidaseFórmula:C12H15NO8Cor e Forma:PowderPeso molecular:301.25 g/molL-Histidine 7-amido-4-methylcoumarin hydrochloride
CAS:L-Histidine 7-amido-4-methylcoumarin hydrochloride is a fluorogenic substrate, which is a synthetic chemical that is used as a substitute for natural substrates. It reacts with enzymes in the presence of hydrogen peroxide to produce light. This product has been used as an enzyme substrate, a diagnostic tool and has been used to detect bacterial growth in culture media. L-Histidine 7-amido-4-methylcoumarin hydrochloride is also used for food testing and environmental testing, where it can be used as a chromogenic substrate or conjugate respectively. This product has CAS number 5981-00-6 and molecular weight of 254.27 g/mol.Fórmula:C16H17ClN4O3Peso molecular:348.79 g/mol
