Substratos enzimáticos

6-Chloro-1H-indol-3-yl b-D-mannopyranoside is an enzyme substrate that is used in the fluorogenic and chromogenic assays for detecting indoleamines. This compound has a conjugate, which is a fluorescent molecule that can be attached to an antibody to target specific proteins or cells. 6-Chloro-1H-indol-3-yl b-D-mannopyranoside is used as a diagnostic agent in the detection of bacterial and fungal infections. It is also used as an indicator of cell growth in culture media.

<p>2-Aminophthalylhydrazido 2-acetamido-2-deoxy-b-D-glucopyranoside is a chromogenic substrate that is used as a diagnostic in biological research. It reacts with enzymes to form fluorescent products, and can be used to detect the presence of bacteria and fungi. This product has a CAS number of 128879-80-1 and can be used for food testing, environmental testing, or bioluminescence. It is an important component in the development of new antibiotics.</p>

<p>Chemiluminescent substrate for beta-D-glucosidase</p>

<p>4-Nitrophenyl 2-O-(α-L-fucopyranosyl)-β-D-galactopyranoside is a chromogenic enzymatic substrate for the detection and quantification of glycosidases, such as fucosidases and galactosidases. Upon enzymatic cleavage, the substrate releases the p-nitrophenol chromophore, which can be quantified via colorimetric analysis. This pNP-conjugated compound enables the rapid, sensitive, and high-throughput detection of enzyme activity in various applications, including enzymatic assays, inhibitor screening, and mechanistic studies. Its precise specificity for α-L-fucopyranosyl and β-D-galactopyranoside moieties makes it an excellent tool for advancing research in glycobiology and related fields.</p>

<p>Aldol® 515 palmitate is a high quality, non-toxic and water soluble chromogenic substrate for enzymes. It is used to detect the presence of lipase activity due to yielding a red color.</p>

<p>Resorufin maltotriose is an alpha-amylase substrate that releases the dye resorufin with red emission (lmax=585 nm) after enzyme reaction.</p>

<p>Phenylethyl b-D-thioglucopyranoside is a modification of the sugar molecule. It is an oligosaccharide that can be synthesized from a complex carbohydrate. Phenylethyl b-D-thioglucopyranoside is a high purity product that can be custom synthesized with high purity and CAS No. It also has methylation, glycosylation, and polysaccharide properties. The product's chemical structure can be modified in order to change the properties of this substance. This product is fluorinated or saccharified.</p>

<p>2-Chloro-4-nitrophenyl b-D-cellobioside (CNPG) is a chromogenic enzyme substrate that is commonly used to detect and measure the activity of β-glucosidases. CNPG is cleaved by the enzyme into the chromogenic product, which can be detected spectrophotometrically. β-glucosidases are important enzymes in the breakdown of cellulose, and CNPG is a widely used substrate for the detection and quantification of these enzymes in various research studies.</p>

<p>2-Nitrophenyl-b-D-glucuronide is a chromogenic substrate used to detect the activity of beta-glucuronidase in various biological samples. Upon hydrolysis by the enzyme, the substrate releases 2-nitrophenol, which can be spectrophotometrically measured at 405 nm. The substrate is commonly used in clinical assays to evaluate liver and kidney function, as well as in drug metabolism studies. It is also used in environmental monitoring to assess the presence of microbial activity in soil and water samples. Overall, 2-Nitrophenyl-b-D-glucuronide is a widely used substrate with excellent sensitivity and specificity, making it a valuable tool in enzymology research and diagnostic testing.</p>

<p>2-Nitrophenyl 2,3,4,6-tetra-O-acetyl-b-D-galactopyranoside is a fluorescent substrate for β-galactosidase. It is used in food testing, environmental testing and diagnostics. This substrate is also used to detect the presence of β-galactosidase activity in cells and tissues.<br>2NPTABG provides a fluorescence signal that can be detected with an ultraviolet light or a luminometer. The luminescence intensity decreases when it reacts with β-galactosidase enzyme.<br>This product has a CAS number of 3053-17-6 and is available at high purity levels.</p>

<p>The AquaSpark® 510 Nitroreductase/NADH Probe (CL-NTR) is a water-soluble probe for the selective chemiluminescent detection of nitroreductase (NTR) activity. It is a versatile tool for monitoring a number of biological processes.</p>

<p>4-Nitrophenyl 3,4-O-isopropylidene-a-D-galactopyranoside is a chemiluminescent substrate for detecting beta-galactosidase activity in bacteria. This product is a high purity, high quality compound that can be used as a culture media and environmental testing agent. It has been shown to be effective in diagnostics, chromogenic substrate, and enzyme substrate reactions. 4NPPG has been shown to be a fluorogenic substrate for beta-galactosidase assay and can be used as food testing agent with conjugate.</p>

<p>1-Naphthyl a-D-glucopyranoside is a chromogenic substrate used to detect the presence of enzymes that catalyze the hydrolysis of glycosidic bonds, such as alpha-glucosidase. When the substrate is cleaved by the enzyme, it produces a colored product that can be visualized and quantified.</p>

<p>5-Octanoylaminofluoroscein di-b-D-glactopyranoside is a chemiluminescent substrate that can be used for the detection of peroxidase activity. It is a bifunctional substrate, which means it has two reactive groups: one at the 5 position and one at the 8 position. The 5-octanoylaminofluorescein reacts with hydrogen peroxide in a 1:1 ratio, emitting light at 490 nm. The 8-position amine reacts with 2,2'-azino-bis(3-ethylbenzthiazoline)6 sulphonic acid (ABTS) to produce an orange color product that acts as a chromogen. The substrate can be used in diagnostics, food testing, and enzyme substrates.</p>

Aldol® 458 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by phosphatases secreted by bacteria grown in liquid media, yielding yellow color and green fluorescence. Aldol® 458 phosphate can also be used in assays involving phosphatase-coupled antibodies in immunoassay applications (ELISA). Aldol® 458 phosphate can be used under aerobic and anaerobic conditions.

<p>4-Methylumbelliferyl N-acetyl-alpha-D-neuraminic acid ammonium is a fluorogenic substrate for neuraminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl N-acetyl-alpha-D-neuraminic acid ammoniumas a substrate for measuring the neuraminidase activity is used for detecting the glycoproteins on the surface of influenza A and B viruses, as well as for sialidosis diagnosis.</p>

<p>2-Nitrophenyl 2,3-di-O-acetyl-4-O-triethylsilyl-b-D-xylopyranoside is a chromogenic substrate for enzmatic assays to measure xylosidase activity. It is a derivative of xylose that can be hydrolyzed by xylosidase to release the yellow-colored 2-nitrophenyl group, which can be detected spectrophotometrically. By modifying the xylose molecule with acetyl and triethylsilyl groups, the enzyme substrate becomes more stable and resistant to degradation. This substrate is commonly used in the study of plant biomass degradation and in the detection of xylosidase activity in microorganisms.</p>

<p>2-Chloro-4-nitrophenyl b-D-xylobioside is a chemiluminescent substrate that is used as a diagnostic agent. It has been used in the identification of gram positive and gram negative bacteria, which are differentiated by the color of the reaction. This substrate has also been used to detect the presence of certain enzymes produced by bacteria. 2-Chloro-4-nitrophenyl b-D-xylobioside has shown to be an effective enzyme substrate for detecting bacterial growth in culture media and is often used as a fluorogenic substrate in food testing.</p>

Aldol® 484 acetate is a chromogenic substrate for carboxylesterases yielding a red precipitate. In contrast to classical indoxyl substrates, Aldol substrates work not only under aerobic but also under anaerobic conditions

<p>The 4-Nitrophenyl 2-acetamido-2-deoxy-4-O-[2-O-(a-L-fucopyranosyl)-b-D-galactopyranosyl]-b-D-glucopyranoside is a specialized pNP enzyme substrate designed to study fucosyl-galactose-dependent enzymes. This chromogenic substrate is valuable for researchers investigating glycosyltransferase activity, structure-function relationships of glycan-binding proteins, and regulation of cellular signaling pathways involving fucose modifications. Its high specificity and sensitivity ensure accurate measurements in diverse experimental setups, and its chromogenic detection system facilitates rapid and reliable analysis.</p>